The human IGF-1 ELISA kit is a solid-phase immunoassay specially designed to quantitatively measure human IGF-1 in serum, plasma, or tissue homogenates. It is based on the Sandwich-ELISA mechanism. IGF-1 in the sample is bound to the capture antibody immobilized on the 96-well strip plate and then sandwiched with the biotinylated IGF-1 antibody. After the addition of HRP-avidin and TMB substrate, the solution in the wells turns blue. The color reaction is stopped by adding the stop solution into the wells, and the color changes from blue to yellow. The color intensity is positively proportional to the IGF-1 bound in the initial step. The IGF-1 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.
IGF-1 plays a pivotal role in fetal development, adolescent growth, and adult tissue homeostasis. Together with IGF-2, IGF-1 is also important in skeletal muscle accrual during development, muscle maintenance, and aging, as well as during muscle injury. IGF-1 also participated in cellular differentiation and reproductive function. It is a survival agent and growth factor for a diverse range of normal and malignant cells. IGF-1 plays a key role in the anti-apoptotic and mitogenic pathways in many cell types. Altered IGF-1 has been linked to obesity and many related diseases. including cardiovascular diseases and cancer.