Human insulin-like growth factors 1,IGF-1 ELISA Kit

Code CSB-E04580h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P05019
Lead Time 3-5 working days
Abbreviation IGF-1
Target Name insulin-like growth factors 1,IGF-1
Alias N/A
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 ng/mL-500 ng/mL
Sensitivity 1.95 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 92-100  
1:2 Average % 102  
Range % 99-105  
1:4 Average % 93  
Range % 85-95  
1:8 Average % 98  
Range % 94-101  
Recovery
The recovery of human IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 91-95  
EDTA plasma (n=4) 96 92-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
500 2.785 2.752 2.769 2.627  
250 2.219 2.189 2.204 2.062  
125 1.388 1.298 1.343 1.201  
62.5 0.702 0.724 0.713 0.571  
31.2 0.434 0.446 0.440 0.298  
15.6 0.307 0.315 0.311 0.169  
7.8 0.228 0.216 0.222 0.080  
0 0.143 0.141 0.142    
References
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Function The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation
Involvement in disease Insulin-like growth factor I deficiency (IGF1 deficiency)
Subcellular Location Secreted
Protein Families Insulin family
Database Links

HGNC: 5464

OMIM: 147440

KEGG: hsa:3479

STRING: 9606.ENSP00000302665

UniGene: Hs.160562

Pathway HIF-1 signaling pathway
MAPK signaling pathway
mTOR signaling pathway
p53 signaling pathway
PI3K-Akt signaling pathway
Focal adhesion
Ras signaling pathway
Signaling pathways regulating pluripotency of stem cells
AMPK signaling
FoxO signaling pathway
Rap1 signaling pathway

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