Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Signal Transduction
Alternative Names
IBP1; IGF I; IGF IA; IGF IB; IGF-I; Igf1; IGF1_HUMAN; IGF1A; IGFI; IGFIA; Insulin like growth factor 1 (somatomedin C) ; Insulin like growth factor 1; Insulin like growth factor IA; Insulin like growth factor IB; Insulin-like growth factor I; Mechano growth factor; MGF; OTTHUMP00000195080; OTTHUMP00000195081; OTTHUMP00000195082; OTTHUMP00000195083; OTTHUMP00000195084; Somatomedia C; Somatomedin C; Somatomedin-C
Species
Homo sapiens (Human)
Expression Region
49-118aa
Target Protein Sequence
GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression vector recombined with the recombinant DNA was transformed into the E.coli for expression. The recombinant DNA resulted from the fusion of the gene coding for the 49-118aa of the human IGF1 protein and the N-terminal 6xHis tag gene. The product was purified and isolated to get the recombinant human IGF1 protein with N-terminal 6xHis tag. The purity of this recombinant IGF1 protein reaches up to 90%. Under SDS-PAGE condition, this recombinant IGF1 protein showed a band with a molecular weight of about 15 kDa on the gel. It may be used in the research of IGF1-participating signal transduction.
IGF-1 is a gene encoding a protein named insulin-like growth factor I (abbreviated IGF1) and belongs to insulin family, which are structurally closely related to pro-insulin. IGF-1 can bind to and activate insulin receptors. The peptide family has two members, IGF-1, the adult form, and IGF-2, the major fetal form. The human IGF-1 gene consists of six exons, including two leader exons, and has two promoters. IGF1 is the major mediator of prenatal and postnatal growth. The disease involved IGF1 is called insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 is produced primarily in liver and serves as an endocrine hormone mediating the action of GH in peripheral tissues such as muscle, cartilage, bone, kidney, nerves, skin, lungs, and the liver itself.