Mouse Interleukin 1β,IL-1β ELISA Kit

Code CSB-E08054m
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P10749
Lead Time 3-5 working days
Abbreviation IL1B
Protein Biological Process 1 Immunity
Target Name interleukin 1, beta
Alias IL-1, IL1-BETA, IL1F2, catabolin|preinterleukin 1 beta|pro-interleukin-1-beta
Species Mus musculus (Mouse)
Protein Biological Process 3 Inflammatory response
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
HGNC 5992
RGD 2891
MGI 96543
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 82-95  
1:2 Average % 93  
Range % 88-98  
1:4 Average % 96  
Range % 94-102  
1:8 Average % 94  
Range % 90-100  
Recovery
The recovery of mouse IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 87-99  
EDTA plasma (n=4) 93 88-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.618 2.541 2.580 2.386  
1000 2.271 2.187 2.229 2.035  
500 1.802 1.669 1.736 1.542  
250 1.233 1.177 1.205 1.011  
125 0.839 0.775 0.807 0.613  
62.5 0.526 0.513 0.520 0.326  
31.25 0.294 0.286 0.290 0.096  
0 0.199 0.188 0.194    
References
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Function Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Subcellular Location Cytoplasm, cytosol, Lysosome, Secreted, exosome, Cytoplasmic vesicle, autophagosome, Secreted
Protein Families IL-1 family
Tissue Specificity Expressed in activated macrophages (at protein level).
Database Links

KEGG: mmu:16176

STRING: 10090.ENSMUSP00000028881

UniGene: Mm.222830

Pathway MAPK signaling pathway
Lipids and Inflammation in Atherogenesis
NF-kappa B signaling pathway
TNF signaling pathway
Necroptosis
Hematopoietic cell lineage
IL-17 signaling pathway
Inflammasome Signaling
NOD-like receptor signaling pathway
Osteoclast differentiation
Th17 cell differentiation
Toll-like receptor signaling pathway
AGE-RAGE signaling pathway in diabetic complications
Excitatory synapse pathway

Q&A and Customer Reviews

Q&A
Q:

I need a Elisa analysis of Interleukins IL 1beta on macrophase cell and lymphocytes.

A:
Thanks for your inquiry.
Regarding elisa kit for mouse Interleukin 1β,we have the following kit for you:
#CSB-E08054m,Mouse Interleukin 1β,IL-1β ELISA Kit
This kit is suitable to detect cell culture supernates.I will send you the kit manual by email.
But for macrophase cell and lymphocytes, sorry we did not test either of them with this kit.We suggest you do pre-testing if you want to test macrophase cell and lymphocytes.
Customer Reviews

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