Mouse Interleukin 1β,IL-1β ELISA Kit

Instructions
Code CSB-E08054m
Size 96T,5×96T,10×96T

Product Details

Target Name interleukin 1, beta
Alternative Names IL-1, IL1-BETA, IL1F2, catabolin|preinterleukin 1 beta|pro-interleukin-1-beta
Abbreviation IL1B
Uniprot No. P10749
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 82-95  
1:2 Average % 93  
Range % 88-98  
1:4 Average % 96  
Range % 94-102  
1:8 Average % 94  
Range % 90-100  
Recovery
The recovery of mouse IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 87-99  
EDTA plasma (n=4) 93 88-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.618 2.541 2.580 2.386  
1000 2.271 2.187 2.229 2.035  
500 1.802 1.669 1.736 1.542  
250 1.233 1.177 1.205 1.011  
125 0.839 0.775 0.807 0.613  
62.5 0.526 0.513 0.520 0.326  
31.25 0.294 0.286 0.290 0.096  
0 0.199 0.188 0.194    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Q&A and Customer Reviews

 Q&A
Q:

I need a Elisa analysis of Interleukins IL 1beta on macrophase cell and lymphocytes.

A:
Thanks for your inquiry.
Regarding elisa kit for mouse Interleukin 1β,we have the following kit for you:
#CSB-E08054m,Mouse Interleukin 1β,IL-1β ELISA Kit
This kit is suitable to detect cell culture supernates.I will send you the kit manual by email.
But for macrophase cell and lymphocytes, sorry we did not test either of them with this kit.We suggest you do pre-testing if you want to test macrophase cell and lymphocytes.
 Customer Reviews

Target Data

Function Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Subcellular Location Cytoplasm, cytosol, Lysosome, Secreted, exosome, Cytoplasmic vesicle, autophagosome, Secreted
Protein Families IL-1 family
Tissue Specificity Expressed in activated macrophages (at protein level).
Database Links

KEGG: mmu:16176

STRING: 10090.ENSMUSP00000028881

UniGene: Mm.222830

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