Pig Interleukin 15(IL-15) ELISA Kit

Instructions
Code CSB-E14320p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 15
Alternative Names IL15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
Abbreviation IL15
Uniprot No. Q95253
Species Sus scrofa (Pig)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 0.78 pg/mL-50 pg/mL
Sensitivity 0.195 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %85
Range %80-89
1:2Average %102
Range %97-107
1:4Average %87
Range %84-91
1:8Average %84
Range %81-89
Recovery
The recovery of pig IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10097-103
EDTA plasma (n=4)8480-88
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
502.602 2.576 2.589 2.400
251.879 1.850 1.865 1.676
12.51.144 1.168 1.156 0.967
6.250.683 0.691 0.687 0.498
3.120.452 0.468 0.460 0.271
1.560.361 0.391 0.376 0.187
0.780.288 0.298 0.293 0.104
00.191 0.186 0.189
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha (By similarity).
Gene References into Functions
  1. Study identified two completely linked SNPs in the porcine IL15 promoter region that could alter IL15 transcription activity. As interleukin-15 can inhibit porcine adipocyte differentiation, these promoter mutations could affect intramuscular fat deposition by producing differential levels of muscle-derived interleukin-15. PMID: 29168191
  2. Myokine IL-15 regulates the crosstalk of co-cultured porcine skeletal muscle satellite cells and preadipocytes. PMID: 25098601
  3. results demonstrate that porcine reproductive and respiratory syndrome virus (PRRSV)infection could induce IL-15 production in macrophages/dendritic cells; data further show that upregulation of IL-15 by PRRSV requires PKC and NF-kappaB pathways PMID: 22573868
  4. IFN-gamma targets the adipocyte to induce IL-15 expression, thus indicating a possible role for the adipocyte in the regulation of T-cell function and muscle metabolism during the innate immune response PMID: 14656772
  5. When induced by IFN-gamma or other inflammatory mediators, IL-15 may be a significant homeorhetic factor that mobilizes and directs energy away from the adipocyte to other cells during the acute phase of the inflammatory response. PMID: 15155279

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Subcellular Location Secreted
Protein Families IL-15/IL-21 family
Database Links

KEGG: ssc:397683

STRING: 9823.ENSSSCP00000009657

UniGene: Ssc.8833

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