Pig Interleukin 6,IL-6 ELISA Kit

Instructions
Code CSB-E06786p
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interleukin 6 (interferon, beta 2)
Alternative Names IL6 ELISA Kit; Interleukin-6 ELISA Kit; IL-6 ELISA Kit
Abbreviation IL6
Uniprot No. P26893
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.25 pg/mL-80 pg/mL
Sensitivity 0.31 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %86
Range %80-90
1:2Average %95
Range %91-100
1:4Average %97
Range %95-103
1:8Average %90
Range %85-95
Recovery
The recovery of pig IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-95
EDTA plasma (n=4)9995-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
802.212 2.101 2.157 2.066
401.725 1.704 1.715 1.624
201.223 1.194 1.209 1.118
100.871 0.809 0.840 0.749
50.519 0.504 0.512 0.421
2.50.332 0.315 0.324 0.233
1.250.192 0.182 0.187 0.096
00.093 0.089 0.091  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity).
Gene References into Functions
  1. IL-6 was low or undetectable in Boar seminal plasma. PMID: 22482797
Subcellular Location Secreted
Protein Families IL-6 superfamily
Database Links

KEGG: ssc:399500

STRING: 9823.ENSSSCP00000024026

UniGene: Ssc.62

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