Pig Interleukin 6,IL-6 ELISA Kit

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Pig Interleukin 6,IL-6 ELISA Kit

Name: Pig Interleukin 6,IL-6 ELISA Kit
Brand: CUSABIO
SKU: CSB-E06786p

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Code	CSB-E06786p
Size	96T,5×96T,10×96T
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Target Data

Target Name

interleukin 6 (interferon, beta 2)

Alternative Names

IL6 ELISA Kit; Interleukin-6 ELISA Kit; IL-6 ELISA Kit

Abbreviation

IL6

Uniprot No.

P26893

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.25 pg/mL-80 pg/mL

Sensitivity

0.31 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-90
1:2	Average %	95
	Range %	91-100
1:4	Average %	97
	Range %	95-103
1:8	Average %	90
	Range %	85-95

Recovery

The recovery of pig IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	99	95-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
80	2.212	2.101	2.157	2.066
40	1.725	1.704	1.715	1.624
20	1.223	1.194	1.209	1.118
10	0.871	0.809	0.840	0.749
5	0.519	0.504	0.512	0.421
2.5	0.332	0.315	0.324	0.233
1.25	0.192	0.182	0.187	0.096
0	0.093	0.089	0.091

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

The beneficial impacts of splanchnic vasoactive agents on hepatic functional recovery in massive hepatectomy porcine model HS Jo,Surgery and Nutrition,2020
Effects of Increasing Doses of Lactobacillus Pre-Fermented Rapeseed Product with or without Inclusion of Macroalgae Product on Weaner Piglet Performance and … GD Satessa,animals,2020
Streptococcus suis Serotype 2 Infection Causes Host Immunomodulation Through Induction of Thymic Atrophy Wang S,Infect Immun,2020
Effects of early protein restriction on the growth performance and gut development of pigs fed diets with or without antibiotic Zhao X,animal,2019
Initial Immune Response in Escherichia coli, Staphylococcus aureus, and Candida albicans Bacteremia Vaios Spyropoulos, et al,Inflammation,2019
Oral administration of Lactobacillus delbrueckii during the suckling period improves intestinal integrity after weaning in piglets Yinghui Li, et al,Journal of Functional Foods,2019
Maternal dietary resveratrol alleviates weaning-associated diarrhea and intestinal inflammation in pig offspring by changing intestinal gene expression and microbiota Qingwei Meng, et al,Food & Function,2019
Dietary xylo-oligosaccharide improves intestinal function in weaned piglets Jie Yin, et al,Food & Function,2019
Cathepsin L promotes secretory IgA response by participating in antigen presentation pathways during Mycoplasma Hyopneumoniae infection Zhang N, et al,PLoS One.,2019
Effects of Clostridium butyricum or in combination with Bacillus licheniformis on the growth performance, blood indexes, and intestinal barrier function of weanling piglets Zong X, et al,Livestock Science,2018
β-hydroxy-β-methylbutyrate modulates lipid metabolism in adipose tissues of growing pigs Yehui Duan.et al,FOOD & FUNCTION,2018
Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1 Qingwei Meng.et al,J Anim Sci Biotechnol.,2018
Oyster crude polysaccharides attenuates lipopolysaccharide-induced cytokines production and PPARr expression in weanling piglets. Yin G.et al,Springerplus.,2016
Conjugated linoleic acid mitigates testosterone-related changes in body composition in male guinea pigs Yang SQ.et al,Nutr Res.,2016
Effects of the antimicrobial peptide cecropin AD on performance and intestinal health in weaned piglets challenged with Escherichia coli. Wu S et al,Peptides,2012
Mouse Model Infected Porcine Circovirus Types 2 Gang Liu et al,Journal of Animal and Veterinary Advances,2012

Function	Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity).
Gene References into Functions	IL-6 was low or undetectable in Boar seminal plasma. PMID: 22482797
Subcellular Location	Secreted
Protein Families	IL-6 superfamily
Database Links	KEGG: ssc:399500 STRING: 9823.ENSSSCP00000024026 UniGene: Ssc.62

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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