Sheep Interleukin 6,IL-6 ELISA Kit

Sheep Interleukin 6,IL-6 ELISA Kit

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Target

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IL6 Antibodies

IL6 Proteins

IL6 Antibodies

IL6 Proteins

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ELISA Kit
Sheep Interleukin 6,IL-6 ELISA Kit

Specification
Target

Code

CSB-E10116Sh

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Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P29455

Lead Time

3-5 working days

Abbreviation

IL6

Protein Biological Process 1

Immunity

Target Name

interleukin 6 (interferon, beta 2)

Alias

Species

Ovis aries (Sheep)

Protein Biological Process 3

Acute phase

Sample Types

serum, plasma, tissue homogenates

Detection Range

2 pg/mL-80 pg/mL

Sensitivity

2 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Competitive

Target Details

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.

HGNC

1834

RGD

2327

MGI

88373

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-98
1:2	Average %	96
	Range %	92-99
1:4	Average %	94
	Range %	89-101
1:8	Average %	92
	Range %	88-98

Recovery

The recovery of sheep IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-103
EDTA plasma (n=4)	95	89-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
0	2.541	2.439	2.490
2	1.382	1.352	1.367
6	0.756	0.785	0.771
18.4	0.405	0.412	0.409
40	0.219	0.224	0.222
80	0.126	0.123	0.125

References

Extracts from Microalga Chlorella sorokiniana Exert an Anti-Proliferative Effect and Modulate Cytokines in Sheep Peripheral Blood Mononuclear Cells Ciliberti MG, et al,Animals,2019
Immune response of sheep naturally infected with Haemonchus spp. on pastures with two different nutritional conditions Gustavo Toscan.et al,SEMIN-CIENC AGRAR,2016
The diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis El-Deeb W M,.et al,PeerJ,2016
Malignant ovine theileriosis: serum concentrations of some inflammatory components and adenosine deaminase activity Razavi S M.et al,Comparative Clinical Pathology,2015
Lipoproteins profile, acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep with pneumonic pasteurellosis El-Deeb W M.et al,Comparative Clinical Pathology,,2015
Comparative Efficacy of the Steroids Administered by Inhalation and Parenteral Route in Lambs with Experimentally Induced Endotoxemia Akin Kirbas. et al,International Journal of Veterinary Science,2015

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Function	Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity).
Subcellular Location	Secreted
Protein Families	IL-6 superfamily
Database Links	KEGG: oas:443406 UniGene: Oar.460
Pathway	HIF-1 signaling pathway Jak-STAT signaling pathway PI3K-Akt signaling pathway TNF signaling pathway Cellular senescence Hematopoietic cell lineage IL-17 signaling pathway NOD-like receptor signaling pathway Th17 cell differentiation Toll-like receptor signaling pathway AGE-RAGE signaling pathway in diabetic complications FoxO signaling pathway Inflammatory Pain

IL6 Antibody

IL6 Protein

IL6 Antibodies for Ovis aries (Sheep)

Code	Product Name	Species Reactivity	Application
CSB-PA011664ZA01SH	IL6 Antibody	Ovis aries	ELISA, WB (ensure identification of antigen)

IL6 Proteins for Ovis aries (Sheep)

Code	Product Name	Source
CSB-EP011664SH	Recombinant Sheep Interleukin-6(IL6)	E.coli
CSB-YP011664SH	Recombinant Sheep Interleukin-6(IL6)	Yeast
CSB-BP011664SH CSB-MP011664SH	Recombinant Sheep Interleukin-6(IL6)	Baculovirus Mammalian cell

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.