Sheep Interleukin 6,IL-6 ELISA Kit

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Target Data

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Sheep Interleukin 6,IL-6 ELISA Kit

Name: Sheep Interleukin 6,IL-6 ELISA Kit
Brand: CUSABIO
SKU: CSB-E10116Sh

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Code	CSB-E10116Sh
Size	96T,5×96T,10×96T
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Target Data

Target Name

interleukin 6 (interferon, beta 2)

Alternative Names

IL6 ELISA Kit; Interleukin-6 ELISA Kit; IL-6 ELISA Kit

Abbreviation

IL6

Uniprot No.

P29455

Species

Ovis aries (Sheep)

Sample Types

serum, plasma, tissue homogenates

Detection Range

2 pg/mL-80 pg/mL

Sensitivity

2 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-98
1:2	Average %	96
	Range %	92-99
1:4	Average %	94
	Range %	89-101
1:8	Average %	92
	Range %	88-98

Recovery

The recovery of sheep IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-103
EDTA plasma (n=4)	95	89-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
0	2.541	2.439	2.490
2	1.382	1.352	1.367
6	0.756	0.785	0.771
18.4	0.405	0.412	0.409
40	0.219	0.224	0.222
80	0.126	0.123	0.125

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Effect of Central Injection of Neostigmine on the Bacterial Endotoxin Induced Suppression of GnRH/LH Secretion in Ewes during the Follicular Phase of the Estrous Cycle Herman A P, et al,International Journal of Molecular Sciences,2019
Acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep, goats and she-camels with Coxiella burnetii infection-induced abortion El-Deeb W, et al,Comparative Immunology, Microbiology and Infectious Diseases,2019
Extracts from Microalga Chlorella sorokiniana Exert an Anti-Proliferative Effect and Modulate Cytokines in Sheep Peripheral Blood Mononuclear Cells Ciliberti MG, et al,Animals,2019
Immune response of sheep naturally infected with Haemonchus spp. on pastures with two different nutritional conditions Gustavo Toscan.et al,SEMIN-CIENC AGRAR,2016
The diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis El-Deeb W M,.et al,PeerJ,2016
Malignant ovine theileriosis: serum concentrations of some inflammatory components and adenosine deaminase activity Razavi S M.et al,Comparative Clinical Pathology,2015
Lipoproteins profile, acute phase proteins, proinflammatory cytokines and oxidative stress biomarkers in sheep with pneumonic pasteurellosis El-Deeb W M.et al,Comparative Clinical Pathology,,2015
Comparative Efficacy of the Steroids Administered by Inhalation and Parenteral Route in Lambs with Experimentally Induced Endotoxemia Akin Kirbas. et al,International Journal of Veterinary Science,2015

Function	Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity).
Gene References into Functions	The aim of the study was to assess the prognostic significance of Tumor Necrosis Factor-alpha, Interleukin-12(p40), Interleukin-6 and Interleukin 10 levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis. PMID: 25817420 Pelibuey breed in grazing areas exhibited different expression of IL-5 and IL-6 obtained from peripheral blood mononuclear cells against Haemonchus contortus, suggesting the importance of these cytokines in regulating the nematode infection. PMID: 26094646 Intravenous injection of SB203580 successfully inhibited synthesis of IL-1beta and reduced the production of IL-6 in the hypothalamus. PMID: 24995301 This is first report of single nucleotide polymorphism of IL-6 gene of Pakistani sheep. PMID: 20878475
Subcellular Location	Secreted
Protein Families	IL-6 superfamily
Database Links	KEGG: oas:443406 UniGene: Oar.460

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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