CHUK Antibody

Code CSB-PA003008
Size US$167
  • Western Blot analysis of 293 cells using IKKα Polyclonal Antibody
  • Western Blot analysis of various cells using IKKα Polyclonal Antibody
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Product Details

Uniprot No. O15111
Target Names CHUK
Alternative Names chuk antibody; CHUK1 antibody; Conserved Helix Loop Helix Ubiquitous Kinase antibody; Conserved helix loop ubiquitous kinase antibody; Conserved helix-loop-helix ubiquitous kinase antibody; I Kappa B Kinase 1 antibody; I Kappa B Kinase Alpha antibody; I-kappa-B kinase 1 antibody; I-kappa-B kinase alpha antibody; IkappaB kinase antibody; IkB kinase alpha subunit antibody; IkBKA antibody; IKK 1 antibody; IKK A antibody; IKK a kinase antibody; IKK-A antibody; IKK-alpha antibody; IKK1 antibody; IKKA antibody; IKKA_HUMAN antibody; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody; Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody; Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody; NFKBIKA antibody; Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody; Nuclear factor NF kappa B inhibitor kinase alpha antibody; Nuclear factor NF-kappa-B inhibitor kinase alpha antibody; Nuclear factor NFkappaB inhibitor kinase alpha antibody; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody; TCF-16 antibody; TCF16 antibody; Transcription factor 16 antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Synthesized peptide derived from Human IKKα around the non-phosphorylation site of T23.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor
Gene References into Functions
  1. evidence that DCNL5 may be involved in innate immunity, as it is a direct substrate of the kinase IKKalpha during immune signalling. PMID: 29958295
  2. cFLIP appears to bind to IKKalpha to prevent IKKalpha from phosphorylating and activating IRF7. PMID: 29222334
  3. functions as a suppressor of lung adenocarcinoma; deletion up-regulates NOX2 and down-regulates NRF2, leading to ROS accumulation and blockade of cell senescence induction PMID: 29311298
  4. HOTAIR operates the action of IKKalpha, IKKbeta, IKKgamma in liver cancer stem cells PMID: 27367027
  5. Results indicate the involvement of IKK and NF-kappaB signaling in the maintenance of glioblastoma stem cell. PMID: 27732951
  6. Loss-of-function of LINC00473 in vivo effectively promoted the regression of Wilms tumour via miR-195/IKKalpha-mediated growth inhibition. PMID: 29159834
  7. Study results provide new insights into the molecular mechanisms of maspin suppression in response to HBx, and revealed nuclear IKKalpha as a prognostic biomarker and a potential therapeutic target to improve the clinical outcome of HBV-associated HCC patients. PMID: 27409165
  8. Data show that IKKalpha directly bind to the promoters of LGR5, in turn, upregulating LGR5 expression through activation of STAT3 signaling pathway during cancer progression. PMID: 27049829
  9. single-particle cryoelectron microscopy (cryo-EM) and X-ray crystal structures of human IKK1 in dimeric ( approximately 150 kDa) and hexameric ( approximately 450 kDa) forms, are reported. PMID: 27851956
  10. results suggest that changes in the relative concentrations of RelB, NIK:IKK1, and p100 during noncanonical signaling modulate this transitional complex and are critical for maintaining the fine balance between the processing and protection of p100. PMID: 27678221
  11. IKKalpha-dependent phosphorylation of S376 stimulated whereas IKKalpha-independent phosphorylation of S484 inhibited RORgammat function in Th17 differentiation. PMID: 28667162
  12. IKKalpha promotes migration through dynamic interactions with the EGF promoter depending on the redox state within cells. PMID: 28122935
  13. the dual regulation of STAT1 by IKKalpha in antiviral signaling suggests a role for IKKalpha in the fine-tuning of antiviral signaling in response to non-self RNA. PMID: 27992555
  14. In epithelial ovarian cancer cells, miR-23a enhances the expression of IKKalpha. The proliferation, migration, and invasion of EOC cells are increased by IKKalpha. PMID: 27537390
  15. TLR signaling led to lower expression of LRRC14. PMID: 27426725
  16. IKKalpha is an important determinant of poor outcome in patients with ER-positive invasive ductal breast cancer and thus may represent a potential therapeutic target PMID: 28006839
  17. The molecular mechanisms involved in IKKalpha-related tumors. [review] PMID: 26323241
  18. this study shows that miR-23a regulated IL-17-mediated proinflammatory mediators expression in rheumatoid arthritis by directly targeting IKKalpha PMID: 27936459
  19. W346 effectively inhibited tumor necrosis factor (TNF-a)-induced NF-kappaB activation by suppressing IKK phosphorylation, inhibiting IkB-a degradation, and restraining the accumulation of NF-kappaB subunit p65 nuclear translocation. W346 also affected NF-kappaB-regulated downstream products involved in cycle arrest and apoptosis. PMID: 26520440
  20. Data indicate a significantly higher quantity of mesenchymal stromal cells (MSCs)was produced from human embryonic stem cells (hESCs) with IkappaB kinase (IKK)/nuclear factor kappa B (NF-kappaB) suppression. PMID: 26972683
  21. Findings indicate that IkappaB kinase inhibitor ACHP can slowdown the accumulation of collagen type I (COL1A1). PMID: 26337045
  22. TRIM22 could interact with IkappaB kinase (IKK)alpha but not IKKbeta and could increase the level and phosphorylation of IKKalpha through its really interesting new gene (RING) and spla-ryanodine receptor (SPRY) domains. PMID: 25510414
  23. IKKalpha is diversely expressed in keratinizing and non-keratinizing carcinomas in the same type of cancer. PMID: 26317791
  24. results indicate that nuclear active IKK is a robust biomarker to predict cutaneous squamous cell carcinoma outcome, and suggest the possibility of targeting IKK activity as a future therapy for treating metastatic cutaneous squamous cell carcinomas PMID: 26094020
  25. We also report that the extra-genomic effects elicited by both ligands, leading to recruitment of active Akt to chromatin, are essential for phosphorylation of serine 10 in histone H3 by IKKa PMID: 25482200
  26. Upon infection, the HCV 3'UTR redistributes DDX3X and IKK-alpha to speckle-like cytoplasmic structures shown to be stress granules. PMID: 25740981
  27. Suppression of PKK expression by RNA interference inhibits phosphorylation of IKKalpha and IKKbeta as well as activation of NF-kappaB in human cancer cell lines; thus, PKK regulates NF-kappaB activation by modulating activation of IKKalpha and IKKbeta. PMID: 25096806
  28. Silencing of IKKa in E2-challenged cells resulted into an increased presence of either 8-oxo-Gs as well as of the base excision repair enzyme 8-oxo-guanine-DNA glycosylase 1. PMID: 24971480
  29. Findings indicated that IKKalpha plays a crucial role as a tumor suppressor that suppresses the invasion, metastasis, and angiogenesis of nasopharyngeal carcinoma (NPC) cells in vitro and correlates with the survival in NPC patients. PMID: 24753359
  30. Authors show that activation of NF-kappaB by Kaposi's sarcoma-associated herpesvirus K15 protein involves the recruitment of NF-kappaB-inducing kinase (NIK) and IKK alpha/beta to result in the phosphorylation of p65/RelA on Ser536. PMID: 25187543
  31. Data show that downstream of Akt protein, IkappaB kinase alpha (IKKalpha) directly phosphorylates mammalian target of rapamycin (mTOR) to drive mTORC1 activation. PMID: 24990947
  32. Survivin-2B promoted autophagy and further regulated cell death by accumulating and stabilizing IKK alpha in the nucleus of selenite treated leukemia cells. PMID: 24556686
  33. IKK complex functions as a key mediator of detachment-induced autophagy and anoikis resistance in epithelial cells. PMID: 23778976
  34. Increases in Ikappa B kinase alpha suppresses the progression of nasopharyngeal carcinoma. PMID: 24075781
  35. That block IKKalpha/beta and EGFR pathways. PMID: 23455325
  36. Data indicate that muscle IKK-alpha protein content was significantly lower in chronic obstructive pulmonary disease (COPD) patients. PMID: 24215713
  37. IKK interacts with rictor and regulates the function of mTORC2 including phosphorylation of AKT (at Serine473) and organization of actin cytoskeleton. PMID: 23872070
  38. roles for p53 and IKKalpha/IKKbeta in non-canonical Notch signaling and IL-6 as a novel non-canonical Notch target gene PMID: 23178494
  39. Data indicate that eight of twelve compounds showed acceptable inhibitory effects on IKKbeta. PMID: 23501112
  40. Results suggest that 13-197 targets IKKbeta and thereby inhibits mTOR and NF-kappaB pathways. PMID: 23444213
  41. Our findings suggest ERLIN1-CHUK-CWF19L1 variants are associated with early stage of fatty liver accumulation to hepatic inflammation. PMID: 23477746
  42. Results demonstrate that IL-8 expression is mediated, at least partly, by IKKalpha PMID: 23894194
  43. Reconstituting irradiated mutant animals with wild-type bone marrow (BM) prevented SCC development, suggesting that BM-derived IKKalpha mutant macrophages promote the transition of IKKalpha(low)K5(+)p63(hi) cells to tumor cells. PMID: 23597566
  44. This review highlights major advances in the studies of the nuclear functions of IKKalpha and the mechanisms of IKKalpha nuclear translocation. Understanding the nuclear activity is essential for targeting IKKalpha for therapeutics. PMID: 23343355
  45. Chemical inhibitors of IKK-alpha suppress HCV infection and IKK-alpha-induced lipogenesis, offering a proof-of-concept approach for new HCV therapeutic development PMID: 23708292
  46. Inhibition of IKKalpha partially rescued p53 levels, while concomitant IKKalpha inhibition fully rescued p53 and regulates MDM2 SUMOylation. PMID: 23032264
  47. H5N1 virus NS1 not only blocks IKKbeta-mediated phosphorylation and degradation of IkappaBalpha in the classical pathway but also suppresses IKKalpha-mediated processing of p100 to p52 in the alternative pathway. PMID: 22891964
  48. The results suggest that inactivation of IKKalpha, followed by Akt and FOXO1 phosphorylation and caspase-3 activation, contributes to zerumbone-induced GBM cell apoptosis. PMID: 23035900
  49. Active nuclear p45-IKKalpha forms a complex with nonactive IKKalpha and NEMO that mediates phosphorylation of SMRT and histone H3. PMID: 23041317
  50. E7 proteins from the cutaneous human papillomavirus types demonstrated interaction with IKKalpha but not with IKKbeta. PMID: 22776252
  51. ETEC preents IkBalpha ubiquitination. PMID: 23027537
  52. The binding of p47 to polyubiquitinated NEMO triggers the lysosomal degradation of NEMO, thereby inhibiting IKK activation. PMID: 22990857
  53. HTLV-1 tax-induced rapid senescence is driven by the transcriptional activity of NF-kappaB and depends on chronically activated IKKalpha and RelA. PMID: 22740410
  54. This article discusses IKK-alpha biological activities and associated molecular events in skin tumor development, which may provide insight into the diagnosis, treatment, and prevention of human squamous cell carcinomas in the future. PMID: 22149351
  55. both IKKalpha and IKKbeta coimmunoprecipitated with Bcl-2 and in vitro kinase assay proved the ability of IKK to phosphorylate Bcl-2. PMID: 22262057
  56. MiR-23b suppresses IL-17-, tumor necrosis factor alpha (TNF-alpha)- or IL-1beta-induced NF-kappaB activation and inflammatory cytokine expression by targeting TAB2, TAB3 and IKK-alpha. PMID: 22660635
  57. Like IKKalpha, TRAF4 is atypical within its family because it is the only TRAF family member to negatively regulate innate immune signaling. IKKalpha's phosphorylation of serine-426 on TRAF4 was required for this negative regulation. PMID: 22547678
  58. NIK activity in nonhematopoietic cells controls Th2 cell development and prevents eosinophil-driven inflammatory disease, most likely using a signaling pathway that operates independent of the known NIK substrate IKKalpha. PMID: 22474019
  59. IKKalpha interacts with and phosphorylates FOXA2, thereby suppressing FOXA2 transactivation activity and leads to decreased NUMB expression, and further activates the downstream NOTCH pathway and promotes cell proliferation and tumorigenesis. PMID: 22196886
  60. Polyubiquitination is involved in HTLV-1 Tax-induced IKK alpha/beta activation. PMID: 21862596
  61. NEMO regulates the nuclear function of IKKalpha underlying diminished NF-kappaB signaling in patients with ectodermal dysplasia with immune deficiency PMID: 22156202
  62. Cigarette smoke and TNFalpha increase NIK levels causing phosphorylation of IKKalpha, which leads to histone acetylation. PMID: 21887257
  63. ABIN-2 acts as a positive regulator of NF-kappaB-dependent transcription by activating IKKalpha. PMID: 21784860
  64. Based on differential activation of IKKalpha and IKKbeta, our findings suggest a different profile in the upstream regulation of the IKK-driven NF-kappaB system in asthmatic patients and patients with COPD. PMID: 21571356
  65. In particular, two negative regulators of NF-kappaB, IkappaBa and A20, were upregulated during poliovirus infection. PMID: 21795344
  66. Inhibition of IKKalpha reduced the wound healing, migration, invasion and cell attachment capabilities of prostate cancer cells. PMID: 21191633
  67. BAG3 promotes the emergence of castration resistant prostate cancer by modulating IKKalpha nuclear translocation. PMID: 21451574
  68. These findings indicate that IKKalpha contributes to the tumor-promoting function of the TGFbeta-SMAD signaling pathway in particular cancers. PMID: 21081648
  69. gamma(1)34.5 recruits both IKKalpha/beta and protein phosphatase 1, forming a complex that dephosphorylates two serine residues within the catalytic domains of IkappaB kinase. PMID: 21248029
  70. results indicate that the DeltaNp63 promoter is activated by the keratinocyte-specific TGF-beta signaling mechanism with Smad2 and IKKalpha in squamous cell carcinomas PMID: 21170261
  71. Compared to normal livers, lower levels of TLR2/4 mRNA and TLR4 and pIKKalpha protein were found in primary biliary cirrhosis and nonalcoholic steatohepatitis, indicating the maintenance of suppressed inflammation and immune tolerance in normal livers. PMID: 20940109
  72. Menaquinone-4 reduced the activation of nuclear factor kappaB and inhibited the phosphorylation of IKKalpha/beta after treatment of cells with LPS. PMID: 20149620
  73. cytosolic Hsp60 is likely to be a regulatory component of IKK complex and a mitochondrial factor that regulates cell survival via NF-kappaB pathway PMID: 20351780
  74. hydroxylation of IkappaBalpha by FIH-1 is inconsequential, at least for the functions we could assay in vitro PMID: 21056038
  75. FOXO3a is in a constant inactive state due to its cytoplasmic localization, and that neither PI3K/Akt nor extra-signal-regulated kinase-specific inhibition resulted in its nuclear translocation. PMID: 20671123
  76. results demonstrate that InlC, by interacting with IKKalpha, dampens the host innate response induced by Listeria during the infection process PMID: 20855622
  77. We identified the molecular defect that causes an autosomal recessive lethal syndrome characterized by multiple fetal malformations, a point mutation in CHUK. PMID: 20961246
  78. IKKalpha rapidly and reversibly translocates to the nucleus in response to elevated CO(2). PMID: 20817876
  79. during human monocyte-macrophage differentiation, expression of the microRNAs miR-223, miR-15a and miR-16 decreased considerably, which led to higher expression of the serine-threonine kinase IKKalpha in macrophages PMID: 20711193
  80. Data show that NIK with mutations in the IKKalpha-targeted serine residues was more stable than wild-type NIK and resulted in increased noncanonical NF-kappaB signaling. PMID: 20501937
  81. Methylation of CpG dinucleotides in the promoter of IkappaBalpha exerts strong influences on the NF-kappaB system and consequently on immune functions of intestinal epithelial cells. PMID: 20378831
  82. Thrombin and collagen induce a feedback inhibitory signaling pathway in platelets involving dissociation of the catalytic subunit of protein kinase A from an NFkappaB-IkappaB complex PMID: 20356841
  83. EBNA1 inhibits the canonical NF-kappaB pathway in carcinoma lines by inhibiting the phosphorylation of IKKalpha/beta. PMID: 20051109
  84. Results show that RKIP has an overall inhibitory effect on the NF-kappaB transcriptional activities via IkappaB kinase complex. PMID: 20043910
  85. the ADAP CARMA1 binding site is required for IKK gamma ubiquitination; both TAK1 and CARMA1 binding sites are required for IkappaB alpha phosphorylation and degradation and NF-kappaB nuclear translocation PMID: 20164171
  86. Data provide analysis of the IKK/NIK inter-relationship and its impact on NF-kappaB control by mathematical modelling. PMID: 19909783
  87. Data support a model in which Notch-1 can activate the transcription of ERalpha-target genes via IKKalpha-dependent cooperative chromatin recruitment of Notch-CSL-MAML1 and ERalpha, which promotes the recruitment of p300. PMID: 19838210
  88. Overexpression of a ubiquitin (Ub) G76A mutant leads to persistent activation of the IKK/NF-kappaB pathway in the absence of extra-cellular stimuli. PMID: 19421711
  89. difference of kinetic mechanisms of heterodimer with IKK-2 compared with IKK and TBK-1 PMID: 11815618
  90. TNF-induced recruitment and activation of the IKK complex require Cdc37 and Hsp90. PMID: 11864612
  91. activity is comparable in normal B cells and B-CLL lymphocytes and is not involved in up-regulation of TRAF1 in B-CLL PMID: 12411322
  92. TNFalpha activation of the NF-kappaB pathway is associated with the inducible binding of TAK1 to TRAF2 and both IKKalpha and IKKbeta PMID: 12547194
  93. IKKalpha and IKKbeta may alter their substrate and signaling specificities to regulate mitogen-induced DNA synthesis through distinct mechanisms PMID: 12589056
  94. IKKalpha involved in COX-2 induction in colorectal tumors PMID: 12771929
  95. New nuclear role of IKK-alpha in modifying histone function that is critical for the activation of NF-kappaB-directed gene expression PMID: 12789342
  96. noncoordinate expression of I kappa B kinases plays a role in determining the cell type-specific role of Akt in NF-kappa B activation. PMID: 14585846
  97. in response to HTLV-1 Tax, IKK2 plays a dominant role in signaling for IkappaBalpha degradation, whereas IKK1 appears to play an important role in enhancing the transcriptional activity of NF-kappaB by promoting RelA/p65 phosphorylation. PMID: 14963024
  98. IKK-alpha controls skeletal and craniofacial morphogenesis by repressing expression of fibroblast growth factor (FGF) family members, such as FGF8, whose expression is specifically elevated in the limb bud ectoderm of IKK-alpha-deficient mice PMID: 15071597
  99. IkappaB kinase alpha (IKKalpha) docks to p100 in NF-kappaB-inducing kinase-induced phosphorylation PMID: 15140882
  100. IKK complex is constitutively activated in ATL cells through a cellular mechanism distinct from that of Tax-mediated IKK activation PMID: 15153339

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Involvement in disease Cocoon syndrome (COCOS)
Subcellular Location Cytoplasm, Nucleus
Protein Families Protein kinase superfamily, Ser/Thr protein kinase family, I-kappa-B kinase subfamily
Tissue Specificity Widely expressed.
Database Links

HGNC: 1974

OMIM: 600664

KEGG: hsa:1147

STRING: 9606.ENSP00000359424

UniGene: Hs.198998

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