HMGB1 Antibody

Datasheet
Code CSB-PA049959
Product Type Polyclonal Antibody
Size US$297
Uniprot No. P09429
Image
  • Western blot analysis of extracts from Jurkat cells, HepG2 cells, COLO205 cells and 293 cells, using HMGB1 antibody.
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunogen Synthesized peptide derived from internal of Human HMGB1.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous levels of total HMGB1 protein.
Tested Applications ELISA,WB;WB:1:500-1:3000
Relevance DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells By similarity.

Wen L., Nucleic Acids Res. 17:1197-1214(1989).
Ferrari S., Genomics 35:367-371(1996).
Rasmussen R.K., Electrophoresis 18:588-598(1997).
Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality Polyclonal
Alias HMG-1; HMG1; High mobility group protein 1; SBP-1; amphoterin
Immunogen Species Homo sapiens (Human)
Target Names HMGB1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury
Subcellular Location Nucleus, Chromosome, Cytoplasm, Secreted, Cell membrane, Peripheral membrane protein, Extracellular side, Endosome, Endoplasmic reticulum-Golgi intermediate compartment
Protein Families HMGB family
Tissue Specificity Ubiquituous. Expressed in platelets (PubMed:11154118).
Database Links

HGNC: 4983

OMIM: 163905

KEGG: hsa:3146

STRING: 9606.ENSP00000343040

UniGene: Hs.434102

Pathway Autophagy
Necroptosis
DNA repair pathway

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