ILK Antibody

Datasheet
Code CSB-PA00999A0Rb
See More Details Free Antibody trial simple
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Immunofluorescence staining of MCF-7 cells with CSB-PA00999A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Western Blot
    Positive WB detected in: Rat heart tissue, Mouse heart tissue
    All lanes: ILK antibody at 5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 52, 45, 37 kDa
    Observed band size: 52 kDa

  • IHC image of CSB-PA00999A0Rb diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Product Details

Full Product Name Rabbit anti-Human ILK Polyclonal antibody
Uniprot No. Q13418
Target Names ILK
Alternative Names Integrin-linked protein kinase (EC 2.7.11.1) (59 kDa serine/threonine-protein kinase) (ILK-1) (ILK-2) (p59ILK), ILK, ILK1 ILK2
Raised in Rabbit
Species Reactivity Human, Mouse, Rat
Immunogen Recombinant Human Integrin-linked protein kinase protein (1-228AA)
Immunogen Species Human
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Receptor-proximal protein kinase regulating integrin-mediated signal transduction
Subcellular Location Cell junction, focal adhesion, Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cell projection, lamellipodium, Cytoplasm, myofibril, sarcomere
Protein Families Protein kinase superfamily, TKL Ser/Thr protein kinase family
Tissue Specificity Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Database Links

HGNC: 6040

OMIM: 602366

KEGG: hsa:3611

STRING: 9606.ENSP00000299421

UniGene: Hs.706355

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