Human integrin-linked kinase 1,ILK-1 ELISA Kit

Code CSB-E10005h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q13418
Lead Time 3-5 working days
Abbreviation ILK
Target Name integrin-linked kinase
Alias DKFZp686F1765, P59,
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 39.06 pg/mL-2500 pg/mL
Sensitivity 9.77 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
HGNC 6040
RGD 620063
MGI 1195267
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ILK-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 84
Range % 80-88
1:2 Average % 97
Range % 92-101
1:4 Average % 90
Range % 86-94
1:8 Average % 105
Range % 100-110
Recovery
The recovery of human ILK-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 87 83-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2500 2.028 2.146 2.087 1.945
1250 1.550 1.620 1.585 1.443
625 1.107 1.168 1.138 0.996
312.5 0.720 0.751 0.736 0.594
156.25 0.413 0.409 0.411 0.269
78.13 0.300 0.286 0.293 0.151
39.06 0.210 0.217 0.214 0.072
0 0.139 0.145 0.142  
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Function Receptor-proximal protein kinase regulating integrin-mediated signal transduction
Subcellular Location Cell junction, focal adhesion, Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cell projection, lamellipodium, Cytoplasm, myofibril, sarcomere
Protein Families Protein kinase superfamily, TKL Ser/Thr protein kinase family
Tissue Specificity Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Database Links

HGNC: 6040

OMIM: 602366

KEGG: hsa:3611

STRING: 9606.ENSP00000299421

UniGene: Hs.706355

Pathway PPAR signaling pathway
Focal adhesion

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