Human integrin-linked kinase 1,ILK-1 ELISA Kit

Instructions
Code CSB-E10005h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name integrin-linked kinase
Alternative Names 59 kDa serine/threonine protein kinase ELISA Kit; 59 kDa serine/threonine-protein kinase ELISA Kit; DKFZp686F1765 ELISA Kit; Epididymis secretory protein Li 28 ELISA Kit; HEL S 28 ELISA Kit; ILK 1 ELISA Kit; ILK 2 ELISA Kit; ILK ELISA Kit; ILK-1 ELISA Kit; ILK-2 ELISA Kit; ILK_HUMAN ELISA Kit; ILK1 ELISA Kit; ILK2 ELISA Kit; Integrin linked kinase 2 ELISA Kit; Integrin linked Kinase ELISA Kit; Integrin linked protein kinase ELISA Kit; Integrin-linked protein kinase ELISA Kit; p59 ELISA Kit; p59ILK ELISA Kit
Abbreviation ILK
Uniprot No. Q13418
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 39.06 pg/mL-2500 pg/mL
Sensitivity 9.77 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ILK-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 84
Range % 80-88
1:2 Average % 97
Range % 92-101
1:4 Average % 90
Range % 86-94
1:8 Average % 105
Range % 100-110
Recovery
The recovery of human ILK-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 87 83-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2500 2.028 2.146 2.087 1.945
1250 1.550 1.620 1.585 1.443
625 1.107 1.168 1.138 0.996
312.5 0.720 0.751 0.736 0.594
156.25 0.413 0.409 0.411 0.269
78.13 0.300 0.286 0.293 0.151
39.06 0.210 0.217 0.214 0.072
0 0.139 0.145 0.142  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Receptor-proximal protein kinase regulating integrin-mediated signal transduction
Gene References into Functions
  1. Using molecular modeling and molecular dynamics simulations, we show that Asp344, Asp352, and Thr356 in kindlin-2 and Arg243 and Arg334 in ILK kinase domain (KD) are important in kindlin-2/ILK complex formation. Mutations that disrupt these interactions abrogate kindlin-2 and ILK colocalization in HeLa cells. PMID: 29237230
  2. High ILK expression is associated with Neuroblastoma. PMID: 29695398
  3. We further performed molecular dynamics simulation for 100ns to see the effect of urea on structural stability of kinase domain of ILK at atomic level. Structural changes with increasing concentrations of urea were analysed, and we observed a significant increase in the root mean square deviation, root mean square fluctuations, solvent accessible surface area and radius of gyration PMID: 29309873
  4. ILK overexpression in human CRC associates with EMT and CSC traits, contributing to tumor progression and chemoresistance. PMID: 29496692
  5. Dermal fibroblast-to-myofibroblast transition sustained by alphavss3 integrin-ILK-Snail1/Slug signaling is a common feature for hypermobile Ehlers-Danlos syndrome and hypermobility spectrum disorders. PMID: 29309923
  6. These results indicated that ILK promoted cell proliferation and growth in breast cancer cells through activation of the PI3K/Akt pathway. PMID: 28791358
  7. ILK-induced epithelial-mesenchymal transition is a novel mechanism in the pathogenesis of adenomyosis PMID: 29409901
  8. Herein, we propose a new ILK-MMP9-MRTF axis that appears to be critical for endothelial-mesenchymal transition differentiation of endothelial to cancer-associated fibroblasts -like cells. Thus, it might be an attractive target for cancer treatment PMID: 28893556
  9. The current data support MT1-MMP as an additional ILK substrate and show that modulation of ILK expression and activity inhibit MT1-MMP-related pro-metastatic behaviors of ovarian cancer cells. PMID: 26959113
  10. Results show that ILK is overexpressed in metastasis of bladder cancer and the up-regulation of ILK could increase cell proliferation, change cell morphology and regulate cell cycle promoting epithelial-mesenchymal transition. PMID: 27576342
  11. This study is the first to identify EMILIN-1 and ILK as prospective markers of islet regenerative function in human mesenchymal stem cells. PMID: 27090767
  12. ILK aids trophoblast syncytialization via downregulation of CDH1, perhaps through an ILK-PARP1-SNAI1 pathway PMID: 28339614
  13. We conclude that ILK is critical for maintaining the collecting duct epithelium and renal function and is a key intermediate for periostin activation of signaling pathways involved in cyst growth and fibrosis in PKD. PMID: 28522687
  14. Emodin inhibits the migration and invasion abilities of human endometrial stromal cells by by facilitating the mesenchymal-epithelial transition through targeting integrin-linked kinase. PMID: 27130230
  15. Data indicate a regulatory role for tetraspanin 8 (Tspan8) in melanoma progression by modulating cell-matrix interactions through beta1 integrin - integrin-linked kinase (ILK) axis and establish Tspan8 as a negative regulator of ILK activity. PMID: 28188308
  16. Our results define ILK as a key mechanotransducer in modulating breast cancer stem-like cells development in response to tissue mechanics and oxygen tension. PMID: 27503933
  17. Overexpression of ILK therefore promoted the proliferation of SHG44 human glioma cells, reduced apoptosis and reduced sensitivity to TMZ via decreasing the activity of caspase3. PMID: 28138714
  18. iNOS-derived NO plays a crucial role during atherosclerosis by regulating the endocytic-lysosomal degradation of ILK in endothelial cells. PMID: 28546219
  19. Using in vitro studies, we demonstrated that ILK and PI3K/AKT inhibitors suppressed the contraction of fibroblast-populated collagen lattices, inhibited fibroblast migration, and interrupted the effect of TGF-beta1 on promoting alpha smooth muscle actin (alpha-SMA) expression in fibroblasts. PMID: 27111285
  20. During human endometrial decidualization, ILK is essential for morphologic transformation of endometrial stromal cells through organization of the actin cytoskeleton. PMID: 28069177
  21. These data suggest a novel link between Tiam1 and RhoG/ILK /ELMO2 pathway as upstream effectors of the Rac1-mediated phagocytic process in trabecular meshwork cells. PMID: 27539661
  22. silencing of ILK attenuates the abnormal proliferation and migration of human Tenon's capsule fibroblasts induced by TGF-beta2. PMID: 27315599
  23. findings describe a TNFalpha-NF-kappaB-mediated mechanism by which ILK expression is induced in the lymph node microenvironment and propose that ILK promotes leukemogenesis by enabling CLL cells to cope with centrosomal defects acquired during malignant transformation PMID: 26837762
  24. High expression of ILK is associated with bladder cancer. PMID: 27683053
  25. ILK overexpression improved the proliferation, metastasis, and invasion ability of colorectal tumor cells, and this effect may be mediated by the NF-kappaB/rel/A signaling pathway. PMID: 27099440
  26. These findings suggest that emodin inhibited the EMT of EOC cells via ILK/GSK-3beta/Slug signaling pathway PMID: 28097141
  27. periostin enhanced invasion and migration abilities of EECs and facilitated the epithelial-mesenchymal transition of endometrial epithelial cell through ILK-Akt signaling pathway PMID: 27034956
  28. High ILK expression is associated with pancreatic ductal adenocarcinomas. PMID: 26887611
  29. Twist-ITGB1-FAK/ILK pathway and their downstream signaling network dictate the Twist-induced EMT process in human mammary epithelial cells and breast cancer cells PMID: 26693891
  30. Results indicated that ILK is imperative in the development and progression of oral squamous cell carcinoma. PMID: 26531674
  31. Periostin enhanced ensometrial stromal cell migration, invasion, and adhesion due to the ILK1/Akt signal pathway. PMID: 25759370
  32. high extracellular concentration of phosphate induced senescence in cultured smooth muscle through the activation of IGF-1 receptor and ILK overexpression PMID: 26467393
  33. our data suggest an essential role of PINCH1, ILK and ILKAP for the radioresistance of p53-wildtype glioblastoma multiforme cells PMID: 26460618
  34. ILK inhibition and knockdown effects senescence in an Rb-dependent manner. PMID: 26176204
  35. ILK may have an important role in the progression of NSCLC, possibly through up-regulation of Snail and MRP1. PMID: 25964055
  36. integrin linked kinase may play a role in cell proliferation, and cell migration in aggressive thyroid cancers. PMID: 26549818
  37. knockdown of ILK inhibits glioma cell migration, invasion and proliferation through upregulation of E-cadherin and downregulation of cyclin D1. Our results suggest that ILK may serve as a promising therapeutic target for glioma. PMID: 25998224
  38. Overexpression of CD29 decreased E-cadherin, but increased fibronectin, vimentin, ILK activity. PMID: 25805567
  39. Overexpression of ILK is associated with arteriosclerosis. PMID: 26210487
  40. our study shows that ILK regulates the EMT in RCC cells, and targeting ILK suppresses invasion and metastasis through inhibition of the EMT in RCC. PMID: 25657336
  41. PARVA promotes metastasis by modulating ILK signalling pathway in lung adenocarcinoma PMID: 25738875
  42. RNA silencing of ILK increases the sensitivity of the A549 lung cancer cell line to cisplatin and promotes its apoptosis. PMID: 25760437
  43. An increase in integrin-linked kinase non-canonically confers NF-kappaB-mediated growth advantages to gastric cancer cells by activating ERK1/2 PMID: 25398317
  44. ILK activity changes may act as a mechanism in response to different stimuli such as H2O2 in the induced TGF-beta1 up-regulation in pathological or even physiological conditions. PMID: 23624332
  45. ILK mediates cardiomyocyte force transduction through regulation of SERCA-2a and phosphorylation of phospholamban. PMID: 25208486
  46. critical function for ILK in mitotic cytoskeletal organization PMID: 24911651
  47. Thymosin beta4 induces invasion and migration of human colorectal cancer cells through the ILK/AKT/beta-catenin signaling pathway. PMID: 25218472
  48. A major ILK binding site in the kindlin-2 FERM domain for regulating cell adhesion has been mapped. PMID: 25160619
  49. Data demonstrate that p34SEI-1 induces the activation of either AKT or ILK signaling on HER2/neu expression status. PMID: 24789658
  50. A pivotal role of ILK in epithelial-mesenchymal transition and metastasis. PMID: 24360977
  51. A novel role for ILK in glioma invasion and metastasis processes via downregulation of CDH1. PMID: 24045896
  52. we conclude that the CRC cells survival and invasion enhanced by hypoxia are mediated by ILK, and ILK may be an important potential therapeutic target for CRC. PMID: 24299190
  53. integrin-linked kinase (ILK) plays a critical role in the suppression of the Hippo pathway via phospho-inhibition of MYPT1-PP1, leading to inactivation of Merlin PMID: 24356468
  54. Study identifies Hsp27 as a novel target of ILK-p38beta signaling complexes, playing a key role in bladder cancer cell migration. PMID: 23435415
  55. Down-regulation of E-cadherin correlated significantly with ILK over-expression. PMID: 23729269
  56. TGF-beta/Smad, integrin/integrin-linked kinase (ILK) and wnt/beta-catenin signaling pathways are involved in epithelial to mesenchymal transition in human renal fibrogenesis. PMID: 24040439
  57. A significant negative correlation between JWA and ILK in melanoma biopsies. PMID: 24064223
  58. RI might play a novel role in the development of bladder cancer through regulating EMT and the ILK signaling pathway. PMID: 23703635
  59. Data indicate that integrin-linked kinase 1 (ILK1) may be an important molecular marker for predicting the carcinogenesis, progression, and prognosis of breast cancer. PMID: 23832543
  60. ILK knockdown attenuates the biological behavior of PCa cells by decreasing Akt activity, demonstrating that ILK is involved in the development and progression of PCa. PMID: 23783575
  61. findings suggest that ILK promotes lung cancer cell migration and invasion via NF-kappaB-mediated upregulation of MMP-9 PMID: 23801886
  62. discusses the recent advances on the structural and functional characterization of ILK and the long-standing debate regarding its reported kinase activity PMID: 23863169
  63. Studied the expression of ILK in clear cell renal carcinomas (CCRC) as a possible prognostic indicator. ILK immunostaining was significantly increased in high-grade in comparison to low-grade CCRCs. PMID: 22814720
  64. ILK overexpression is associated with tumor progression and a poor prognosis in CRC patients and may represent a novel potential prognostic marker for patients with CRC. PMID: 23108908
  65. It was found that ILK downregulation significantly decreased angiogenesis, increased apoptosis, and decreased invasiveness of osteosarcoma cells. PMID: 23784942
  66. ILK could be a novel diagnostic and therapeutic target for tongue cancer. PMID: 23707970
  67. the targeting of ILK with siRNA significantly inhibited cell attachment, spreading, migration and microfilament dynamics in corpus cavernosum smooth muscle cells PMID: 22616551
  68. Down-regulation of integrin-linked kinase reduced the metastatic potential of gastric tumor cells by regulating metastasis-related gene expression, by inhibiting the Akt pathway as well as the activity of transcription factors PMID: 23748822
  69. ILK is necessary for normal oligodendrocyte development and reinforces the bridge between actin cytoskeleton and cell membrane. PMID: 23739974
  70. FAK expression was positively correlated with ILK expression but negatively correlated with PTEN expression in SACC tissues. FAK and ILK expression was positively associated with advanced stage PMID: 23186335
  71. ILK promotes lung cancer cell migration and invasion through the induction of EMT process PMID: 22683084
  72. study suggested that positive ILK and PRDX1 expressions are closely related to the progression and poor prognosis of gallbladder cancer PMID: 23065574
  73. ILK is a therapeutically targetable mediator of ERG-induced epithelial-to-mesenchymal transition (EMT) and transformation in prostate cancer. PMID: 23027626
  74. findings show ILK gene transcription and protein expression may be involved in the development of bladder transitional cell carcinoma PMID: 22938462
  75. demonstrate a requirement for ILK function in TGFbeta-1-induced EMT in mammary epithelial cells and identify the ILK/Rictor complex as a potential molecular target for preventing/reversing EMT PMID: 22310280
  76. ILK identified as a new partner for ADAM12L cellular functions; data suggest a new role for ADAM12L in mediating the functional association of ILK with beta1 integrin to regulate cell adhesion/survival through a PI3K/Akt signaling pathway PMID: 22767580
  77. Downregulation of integrin-linked kinase inhibited cell proliferation and induced apoptosison retinoblastoma cells. PMID: 22490953
  78. results suggest ILK may have an important role in progression and metastasis of oral squamous cell carcinoma, possibly through epithelial-mensenchymal transition involving up-regulation of Snail and consequent aberrant expression of E-cadherin and N-cadherin PMID: 22638656
  79. Through its association with ELMO2, ILK plays key roles in the regulation of Rho GTPases and cross-talk pathways between adhesion and growth factor receptors. PMID: 22568984
  80. ILK represents a novel regulatory checkpoint during human cardiomyogenesis PMID: 22666394
  81. ILK does not play a prominent role in the promotion or sustenance of established multiple myeloma. PMID: 22658851
  82. Down-regulated miR-625 suppresses invasion and metastasis of gastric cancer by targeting ILK PMID: 22677169
  83. Suggest that activation of ILK activated the Wnt/beta-catenin signaling pathway in damaged podocytes in focal segmental glomerulosclerosis. PMID: 21928230
  84. Data show that integrin linked kinase (ILK) over-expression could promote cell proliferation, inhibit cell apoptosis and increase mitosis. PMID: 22482400
  85. results also identify the integrin-linked kinase (ILK) and alpha-parvin proteins as a new molecular partner and target, respectively, of the Lnk adaptor. PMID: 22441983
  86. Knockdown of ILK inhibited bladder cancer proliferation and invasion, changed cell morphology, more importantly, suppressed bladder cancer epithelial-mesenchymal transition as well as tumor growth and metastasis. PMID: 22570869
  87. ILK mainly expressed in scar tissue with formation time shorter than 6 months, and it may affect scar vascularization by regulating gene expressions of KDR and Flt-1. PMID: 22340785
  88. IGFBP requires ILK to induce cell motility. The IGFBP2/integrin/ILK/NF-kappaB network functions as a physiologically active signaling pathway in vivo by driving glioma progression PMID: 22345562
  89. Integrin-linked kinase regulates vasomotor function by preventing endothelial nitric oxide synthase uncoupling in atherosclerosis. PMID: 22194624
  90. the clinical relevance of ILK in hepatocarcinogenesis PMID: 21347395
  91. ILK protein was expressed in myeloma cell lines and kinase activity was elevated when cocultured with bone marrow stromal cells PMID: 20625942
  92. Knocking down ILK gene expression with siRNA inhibited the elevation of VEGF and ICAM-1 induced by high glucose treatment in retinal pigment epithelium cells. PMID: 21553049
  93. Wild type integrin-linked kinase (ILK) reversed the poor mobility of CREG knock-down HUVECs. PMID: 21939655
  94. results suggest that ILK gene transfection is an effective approach to augment angiogenic properties of EPCs in vitro and providing basis for clinical cell-based gene therapy in patients with pre-eclampsia PMID: 21688303
  95. In osteoblasts exposed to dexamethasone, ILK expression was slightly increased, whereas there was substantial reduction in that of beta1 integrin. PMID: 21639857
  96. Our study suggests that ILK is overexpressed in invasive bladder cancer and plays an important role in the EMT of bladder cancer via the control of E-cadherin and MMP-9 expression. PMID: 21351095
  97. the N-terminus of myopodin binds integrin-linked kinase (ILK) both in vivo and in vitro PMID: 21643011
  98. Data show that targeting ILK with siRNA suppresses the growth of gastric cancer cells both in vitro and in vivo, suggesting that ILK plays an important role in gastric cancer progression. PMID: 21941415
  99. ILK promoted proliferation and suppressed apoptosis via repressing phosphorylations of the JNK signal pathway in human retinoblastoma cells. PMID: 21234586
  100. ILK might be involved in the development of bladder cancer, and could serve as a novel potential therapy target for human bladder cancer PMID: 21601006

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Subcellular Location Cell junction, focal adhesion, Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cell projection, lamellipodium, Cytoplasm, myofibril, sarcomere
Protein Families Protein kinase superfamily, TKL Ser/Thr protein kinase family
Tissue Specificity Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Database Links

HGNC: 6040

OMIM: 602366

KEGG: hsa:3611

STRING: 9606.ENSP00000299421

UniGene: Hs.706355

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