Code | CSB-RA963626A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IF | 1:20-1:200 |
IP | 1:200-1:1000 |
The ILK recombinant monoclonal antibody is generated using protein technology and DNA recombinant technology. In the first step, a synthesized peptide derived from human ILK is used to immunize mice. After a certain period, the spleen is removed under sterile conditions for total RNA extraction. The cDNA synthesized through RNA reverse transcription serves as a template for PCR amplification of the ILK antibody gene. The obtained ILK antibody gene is then inserted into a vector, which is transfected into host cells for culture. The ILK recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. It undergoes rigorous verification and can be used in ELISA, WB, IF, and IP experiments for detecting human and mouse ILK protein.
Integrin-linked protein kinase (ILK) is a serine/threonine protein kinase that interacts with the cytoplasmic tails of integrins and other proteins, such as PINCH and Parvin, to form the ILK-PINCH-Parvin (IPP) complex, which plays an important role in the regulation of cell adhesion, migration, proliferation, and survival. ILK also acts as a key signaling hub that integrates extracellular signals from the ECM and other signaling pathways to regulate various cellular processes, including cell adhesion, migration, proliferation, and survival.
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