PPP2CB Antibody

Code CSB-PA00985A0Rb
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western blot
    All lanes: Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Antibody at 2μg/ml + EC109 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/15000 dilution
    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00985A0Rb at dilution of 1:50

  • Immunofluorescent analysis of Hela cells using CSB-PA00985A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • IHC image of CSB-PA00985A0Rb diluted at 1:260 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA00985A0Rb diluted at 1:260 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA00985A0Rb at dilution of 1:100

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) PPP2CB Polyclonal antibody
Uniprot No. P62714
Target Names PPP2CB
Alternative Names PP2A beta antibody; PP2A-beta antibody; PP2AB_HUMAN antibody; PP2Abeta antibody; PP2CB antibody; Ppp2cb antibody; Protein phosphatase 2 (formerly 2A); catalytic subunit; beta isoform antibody; Protein phosphatase 2 catalytic subunit beta isozyme antibody; Protein phosphatase 2; catalytic subunit; beta isoform antibody; Protein phosphatase 2A catalytic subunit beta isoform antibody; Protein phosphatase type 2A catalytic subunit antibody; Serine/threonine protein phosphatase 2A catalytic subunit beta antibody; Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform protein (1-309AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:500
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
Gene References into Functions
  1. The GAS41-PP2Cbeta complex dephosphorylates p53 at serine 366 and regulates its stability PMID: 21317290
  2. Claspin may be one of the phosphoproteins through which PP2A(Aalpha/Cbeta) affects Chk1 phosphorylation when ATR is activated by human immunodeficiency virus-1 Vpr. PMID: 17210576
  3. These data strongly support a recent proposal that a segment at 8p21.3 contains crucial prostate cancer tumor suppressors. PMID: 18460741
Subcellular Location Cytoplasm, Nucleus, Chromosome, centromere, Cytoplasm, cytoskeleton, spindle pole
Protein Families PPP phosphatase family, PP-1 subfamily
Database Links

HGNC: 9300

OMIM: 176916

KEGG: hsa:5516

STRING: 9606.ENSP00000221138

UniGene: Hs.491440

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