Phospho-E2F1 (Thr433) Antibody

Datasheet
Code CSB-PA142360
Product Type Polyclonal Antibody
Size US$360
Uniprot No. Q01094
Image
  • Western blot analysis of extracts from Mouse brain tissue using E2F1 (Phospho-Thr433) Antibody.The lane on the left is treated with the antigen-specific peptide.
Immunogen Peptide sequence around phosphorylation site of Threonine 433(D-L-T(p)-P-L) derived from Human E2F1.
Raised in Rabbit
Species Reactivity Human,Mouse
Specificity The antibody detects endogenous level of E2F1 only when phosphorylated at Threonine 433.
Tested Applications ELISA,WB;WB:1:500-1:1000
Relevance Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias E2F-1, PBR3, RBAP-1, RBBP-3
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Target Names E2F1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters
Subcellular Location Nucleus
Protein Families E2F/DP family
Database Links

HGNC: 3113

OMIM: 189971

KEGG: hsa:1869

STRING: 9606.ENSP00000345571

UniGene: Hs.654393

Pathway Cell cycle
Cellular senescence

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