Recombinant Bovine Rho-associated protein kinase 1 (ROCK1)

Code CSB-YP847572BO
MSDS
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Source Yeast
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Code CSB-EP847572BO
MSDS
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Source E.coli
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Code CSB-EP847572BO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP847572BO
MSDS
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Source Baculovirus
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Code CSB-MP847572BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ROCK1
Uniprot No.
Alternative Names
ROCK1; Rho-associated protein kinase 1; EC 2.7.11.1; Rho-associated; coiled-coil-containing protein kinase 1; Rho-associated; coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK; Fragment
Species
Bos taurus (Bovine)
Expression Region
1-441
Target Protein Sequence
NSKSQMDKDY YQLQAVLEAE RRDRGHDSEK IGDLQARITS LQEEVKHLKH NLERVEGERK EAQDMLNHSE KEKNNLEIDL NYKLKSLQQR LEQEVNEHKV TKARLTDKHQ SIEEAKSVAM CEMEKKLKEE RDAREKAENR VVQIEKQCSM LDVDLKQSQQ KLEHLIENKD RMEDEVKNLT LQLEQESNKR LLLQNELKTQ AFEADNLKGL EKQMKQEINT LLEAKRLLEF ELAQLTKQYR GNEGQMRELQ DQLEAEQYFS TLYKTQVKEL KEEIEEKNRE NLKKIQELQS EKETLATQLD LAETKAESEQ LARGLLEEQY FELTQESKKA ASRNRQEITD KDHTLSRLEE TNSMLTKDIE LLRKENEELT DKMRKAEEEY KLKKEEEINI LKAAFEKNIN TERTLKTQAV NKLAEIMNRK DFKIDRKKAN TQDLRKKKKK K
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles.
Subcellular Location
Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle.
Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family
Database Links
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