Recombinant Human Inhibitor of nuclear factor kappa-B kinase subunit beta(IKBKB)

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Code CSB-EP011572HU
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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names IKBKB
Uniprot No. O14920
Research Area Cancer
Alternative Names I kappa B kinase 2; I kappa B kinase beta; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK beta; IKK-B; IKK-beta; IKK2; IKKB; IKKB_HUMAN; IMD15; Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-756aa
Target Protein Sequence MSWSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLAERLEKWLQLMLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQSFTALDWSWLQTEEEEHSCLEQAS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 92.5 kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus.
Gene References into Functions
  1. The results demonstrate that the inhibition of miR-16 renders resistance to paclitaxel in vitro and in vivo by targeting IKBKB via NF-kappa B signaling pathway. PMID: 29935185
  2. Expression of TAp63, IKKbeta and XBP1s is also increased in livers of obese patients with liver steatosis. PMID: 28480888
  3. IKK2 and NF-kappaB are related to poor prognosis and are potential predictors of response to platinum-based chemotherapy in high grade serous carcinoma PMID: 29254797
  4. These studies identify functional EREs in the IKKB promoter and identify IKKB as an ERalpha and NSC35446.HCl-regulated gene, and they suggest that NF-kappaB and IKKB, which were previously linked to antiestrogen resistance, are targets for NSC35446.HCl in reversing antiestrogen resistance. PMID: 28808806
  5. Curcumin suppressed CXCL5 expression by direct inhibition of IKKbeta phosphorylation, and inhibition of p38 MAPK via induction of negative regulator MKP-1. PMID: 27538525
  6. This study demonstrated that nerve injury-induced Csf1 upregulation was ameliorated indicating that IKK/NF-kappaBeta-dependent SGC activation induced Csf1 expression in sensory neurons. PMID: 28722693
  7. Findings uncover a novel function for IKKbeta/mHTTx1 interactions in regulating IL-34 production, and implicate a role for IL-34 in non-cell-autonomous, microglial-dependent neurodegeneration in HD. PMID: 28973132
  8. HOTAIR operates the action of IKKalpha, IKKbeta, IKKgamma in liver cancer stem cells PMID: 27367027
  9. These results suggested that APN ameliorated endothelial inflammation and IR through ROS/IKKbeta pathway. PMID: 27639126
  10. p300-dependent histone H3 acetylation and C/EBPbeta-regulated IKKbeta expression contribute to thrombin-induced IL-8/CXCL8 expression in human lung epithelial cells. PMID: 28428115
  11. EGFR/PI3K/Akt/mTOR/IKK-beta/NF-kappaB signaling promotes head and neck cancer progression. PMID: 26895469
  12. our results illustrate a new direction in nitrosourea treatment, and reveal that the combination of ROS-inducing IKKbeta inhibitors with nitrosoureas can be potentially exploited for melanoma therapy PMID: 28107677
  13. Smad7 expression in necrotizing enterocolitis macrophages interrupts TGF-beta signaling and promotes NF-kappaB-mediated inflammatory signaling in these cells through increased expression of IKK-beta PMID: 26859364
  14. High IKBKB expression is associated with prostate cancer. PMID: 27577074
  15. Akt2, Erk2, and IKK1/2 phosphorylate Bcl3, converting Bcl3 into a transcriptional coregulator by facilitating its recruitment to DNA. PMID: 28689659
  16. Rare variants in IKBKB are associated with decreased waist-to-hip ratio in European-Americans. PMID: 26757982
  17. pVHL mediates K63-linked ubiquitination of IKKbeta, which plays a role in the regulation of IKK/NF-kappaB signalling. PMID: 27693634
  18. our results demonstrate that miR-200b, a transcriptional target of NF-kappaB, suppresses breast cancer cell growth and migration, and NF-kappaB activation, through downregulation of IKBKB, indicating that miR-200b has potential as a therapeutic target in breast cancer patients. PMID: 26433127
  19. The present research concluded that aspirin suppressed prostate cancer cell invasion by reducing MMP-9 activity and uPA expression through decreasing of IKK-beta-mediated NF-kappaB activation, indicating that the ability of aspirin to inhibit cell invasion might be useful in the chemoprevention of metastatic prostate cancer. PMID: 28278500
  20. miR-429 is involved in regulation of the NF-kappaB pathway by targeting IKKbeta and functions as a tumor suppressor in cervical carcinogenesis. PMID: 27883176
  21. TLR signaling led to lower expression of LRRC14. PMID: 27426725
  22. results uncover a previously unidentified role of IKKbeta in regulating glycolysis, sensing low-glutamine-induced metabolic stress, and promoting cellular adaptation to nutrient availability. PMID: 27585591
  23. data clearly demonstrate that KLHL21 negatively regulates TNFalpha-activated NF-kappaB signaling via targeting IKKbeta, providing new insight into the mechanisms underlying NF-kappaB regulation in cells. PMID: 27387502
  24. Cis- and trans-gnetin H suppress cytokine response in LPS-stimulated THP-1 cells by preventing activation of key signaling molecules, IKK-beta, IkappaB alpha, and p65, involved in the NF-kappaB pathway. PMID: 27196294
  25. The neuroprotective effects of celastrol and its analogues may be related to IKKbeta inhibition. PMID: 27931154
  26. Survivin overexpression activates NFkappaB p65, which is important in the acquisition and maintenance of the oncogenic characteristics of esophageal squamous cell carcinoma. PMID: 26718331
  27. Over-expressed IKK-Beta inhibits cell apoptosis in laryngeal squamous cell carcinoma. PMID: 26914121
  28. DAT stabilized IkBa by inhibiting the phosphorylation of Ika by the IkB kinase (IKK) complex. DAT induced proteasomal degradation of TRAF6, and DAT suppressed IKKb-phosphorylation through downregulation of TRAF6 PMID: 26647777
  29. IFIT5 promotes SeV-induced IKK phosphorylation and NF-kappaB activation by regulating the recruitment of IKK to TAK1. PMID: 26334375
  30. Down-regulation of IKBKB expression and NFkappaB signaling in microglia/macrophages infiltrating glioblastoma correlates with defective expression of immune/inflammatory genes and M2 polarization that may result in the global impairment of anti-tumor immune responses in glioblastoma. PMID: 26427514
  31. Combining bortezomib with IKK inhibitor is effective in treating ovarian cancer. PMID: 26267322
  32. MyD88s is positively regulated by IKKbeta and CREB and negatively regulated by ERK1/2 signaling pathways. PMID: 26669856
  33. IKK-beta suppresses GLI1 ubiquitination. PMID: 26603838
  34. we demonstrate that one miR-497, is a likely negative regulator of IKKbeta PMID: 26020802
  35. In cells with functional KEAP1, RTA 405 increased NRF2 levels, but not IKKb or BCL2 levels, and did not increase cell proliferation or survival. PMID: 26301506
  36. findings indicate that the IKBKB and POLB SNPs confer no genetic predisposition to SLE risk in this Chinese Han population PMID: 26167925
  37. Demonstrate the essential role of EGFR/Akt/IkappaBbeta/NF-kappaB pathway in the inhibitory effect of PA-MSHA on invasion and metastasis of HCC through suppressing EMT. PMID: 25066210
  38. identified IKK-beta as a kinase capable of phosphorylating threonine 3 in N-terminal hungtingtin fragments PMID: 26106822
  39. The activation of NF-kappaB induced translocation of AMAP1 to cytoplasm from cell membrane and nucleus, which resulted in augmented interaction of AMAP1 and IKKbeta PMID: 24865276
  40. High IKBKB expression is associated with inflammation in heart Valve Diseases. PMID: 25630970
  41. These studies not only reinforce the significance of maintaining a homeostatic balance of eNOS and IKKbeta within the cell system that regulates NO production, but they also confirm that the IKKbeta-Hsp90 interaction is favored in a high-glucose environment, leading to impairment of the eNOS-Hsp90 interaction, which contributes to endothelial dysfunction and vascular complications in diabetes. PMID: 25652452
  42. IKKbeta regulates endothelial thrombomodulin in a Klf2-dependent manner PMID: 25039491
  43. Studies indicate that transcription factor NF-kappaB plays a key role in numerous physiological processes, and its activation is tightly controlled by a kinase complex, IkappaB kinase (IKK). PMID: 25432706
  44. Suppression of PKK expression by RNA interference inhibits phosphorylation of IKKalpha and IKKbeta as well as activation of NF-kappaB in human cancer cell lines; thus, PKK regulates NF-kappaB activation by modulating activation of IKKalpha and IKKbeta. PMID: 25096806
  45. IKBKB-rs3747811AT single nucleotide polymorphism was associated with a significantly increased risk of developing wheezing. PMID: 25326706
  46. IKKbeta is an IRF5 kinase that instigates inflammation PMID: 25326420
  47. IKKbeta activates two "master" transcription factors of the innate immune system, IRF5 and NF-kappaB PMID: 25326418
  48. RTK-mediated Tyr phosphorylation of IKKbeta has the potential to directly regulate NFkappaB transcriptional activation. PMID: 24386391
  49. Expression of IKBKB gene reduced the cisplain sensitivity of A549 cells. PMID: 24854552
  50. Gain-of function or knockdown of miR-200c in leiomyoma smooth muscle cells (LSMC) regulated IL8 mRNA and protein expression through direct targeting of IKBKB and alteration of NF-kB activity. PMID: 24755559

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Involvement in disease Immunodeficiency 15 (IMD15)
Subcellular Location Cytoplasm. Nucleus. Membrane raft.
Protein Families Protein kinase superfamily, Ser/Thr protein kinase family, I-kappa-B kinase subfamily
Tissue Specificity Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
Database Links

HGNC: 5960

OMIM: 603258

KEGG: hsa:3551

STRING: 9606.ENSP00000430684

UniGene: Hs.597664

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