Name: Recombinant Human Ras-related C3 botulinum toxin substrate 1(RAC1)
Brand: CUSABIO
SKU: 019242HU

Product Details

Purity	>85% (SDS-PAGE)
Target Names	RAC1
Uniprot No.	P63000
Alternative Names	Cell migration inducing gene 5 protein; Cell migration-inducing gene 5 protein; MGC111543; MIG5; Migration inducing gene 5; Migration inducing protein 5; p21 Rac1 ; p21-Rac1; Rac 1; RAC1; RAC1_HUMAN; Ras like protein TC25; Ras related C3 botulinum toxin substrate 1 (rho family; small GTP binding protein Rac1); Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1; Rho family small GTP binding protein Rac1; TC 25; TC25
Species	Homo sapiens (Human)
Expression Region	1-189
Target Protein Sequence	MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPNTPI ILVGTKLDLR DDKDTIEKLK EKKLTPITYP QGLAMAKEIG AVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKRKRKC
Protein Length	Full length protein
Tag Info	The following tags are available. N-terminal His-tagged Tag-Free The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form	Lyophilized powder
Buffer before Lyophilization	Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet	Please contact us to get it.

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Target Background

Function (From Uniprot)	Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In neurons, is involved in dendritic spine formation and synaptic plasticity. In hippocampal neurons, involved in spine morphogenesis and synapse formation, through local activation at synapses by guanine nucleotide exchange factors (GEFs), such as ARHGEF6/ARHGEF7/PIX. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3. In neurons, plays a crucial role in regulating GABA(A) receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in PAK1 activation and eventually F-actin stabilization.; Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
Gene References into Functions	Authors found that miR-885-5p was a direct target of hsa_circ_0004458, and silencing of hsa_circ_0004458 inhibited PTC cell proliferation by miR-885-5p. Authors also demonstrated that RAC1 was a direct target of miR-885-5p and silencing of RAC1 suppressed PTC cell proliferation. PMID: 30086127 MiR-142-3p promotes cellular invasion in colorectal cancer cells by activating RAC1. PMID: 30064309 A functional interplay between HACE1 and Rac1 in cancer, is reported. PMID: 28317937 Results suggest that transient receptor potential vanilloid 4 (TRPV4) accelerates glioma migration and invasion through the AKT/Rac1 signaling, and TRPV4 might be considered as a potential target for glioma therapy. PMID: 29928875 The spatial extent of Rho GTPases gradients governs cell migration, a sharp Cdc42 gradient maximizes directionality while an extended Rac1 gradient controls the speed. PMID: 30446664 that Rac1 plays a major role in the phosphorylation of HACE1 downstream CNF1 toxin. PMID: 29362425 Data indicate that the major mechanism is the ability of p140Cap to interfere with ERBB2-dependent activation of Rac GTPase-controlled circuitries. PMID: 28300085 the B-cell receptor BR3 modulates cellular branching via Rac1 during neuronal migration PMID: 27436754 Results show that in cisplatin-resistant cervical cancer tissues, Rac1, and Wave2 mRNA expression was significantly up-regulated compared to that of the cisplatin-sensitive cervical cancer tissues. In HeLa and Caski cervical cancer cell lines, Rac1 activity and Wave2 protein expression was significantly promoted by SH3BP1 overexpression. PMID: 28786507 RCC2 physically interacts and deactivates a small GTPase Rac1 that is known to be involved in metastasis. PMID: 28869598 Once activated, c-Abl kinase regulated the activity of Vav1, which further affected Rac1/PAK1/LIMK1/cofilin signaling pathway. PMID: 29058761 The effects of Tiam1 on metastasis and EMT mediated by the Wnt/beta-catenin pathway were reversed by Rac1 silencing, suggesting that the prometastatic effect of Tiam1 is mediated by the activation of Rac1. These results indicate that Tiam1 may be a prognostic factor and potential therapeutic target for the treatment of thyroid cancers. PMID: 29277502 RAC1 is associated with giant cell tumor of bone recurrence, which might serve as biomarker for giant cell tumor of bone recurrence PMID: 29651441 These findings highlight a regulatory pathway of Tiam1/Rac1 in Th17 cells and suggest that it may be a therapeutic target in multiple sclerosis. PMID: 27725632 Rac1 is a novel therapeutic target in mantle cell lymphoma. PMID: 29434202 RCC2 regulates apoptosis by blocking Rac1 signaling. RCC2 expression in tumor can be a useful marker for predicting chemotherapeutic response. PMID: 29321004 Silencing Rac1 suppressed the growth and migration of Hypopharyngeal Squamous Cell Carcinoma through the P38 MAPK signaling pathway. PMID: 29410394 Iranian study did not show any association between the studied RAC1 SNPs and ulcerative colitis. PMID: 28412192 cyclin D1 was downregulated, whereas Bcell lymphoma 2associated agonist of cell death (BAD) was upregulated following RAC1 knockdown in colon cancer cells. PMID: 29286138 Pharmacological inhibition of RAC1 could significantly inhibit the proliferation of both RT4 cells and human NF2-associated primary schwannoma cells by inducing apoptosis. Pharmacological inhibition of RAC1 effectively reduced Rac1 activity and down-regulated the pathway downstream of Rac. Moreover, pharmacological inhibition of RAC1 showed a potential antitumor effect, with low toxicity in vivo. PMID: 28934903 Rac1 plays a role in CXCL12 but not CCL3-induced chemotaxis. PMID: 29050986 we revisited the question of the relationship between Cav1 and Stat3-ptyr705 in non-transformed mouse fibroblasts and in human lung carcinoma cells, by examining their effect at different cell densities.Our results demonstrate that Cav1 downregulates cadherin-11, by a mechanism which requires the Cav1 scaffolding domain. This cadherin-11 downregulation, leads to a reduction in cRac1 and Stat3 activity levels. PMID: 29458077 studies revealed that alpha-Syn(A53T) inhibited PDGF-induced Rac1 activation, whereas Cdc42 activation remained unaffected, resulting in unbalanced actin filament remodeling PMID: 27886249 Endogenous Rac1 is critical for the recruitment of FMNL2 to newly forming junctions as well as within already established epithelial sheets. PMID: 29579104 Data show that Ras-like without CAAX 1 protein (RIT1) binds the RHO GTPases CDC42 and RAC1, both of which are crucial regulators of actin dynamics upstream of PAK1. PMID: 29734338 Glucotoxicity promotes aberrant activation and mislocalization of Rac1 and metabolic dysfunction in pancreatic islet beta-cells. PMID: 28828705 This review describes the current knowledge regarding Rac1 pathway deregulation and its association with chemoresistance, radioresistance, resistance to targeted therapies and immune evasion. [review] PMID: 29548483 Silencing of RAC1 did not affect ARF1 recruitment to the leading edges in neutrophil chemotaxis. PMID: 28969640 Surprisingly, Rac1 is not located at the binding site on the Sra1 subunit of the WAVE regulatory complex previously identified by mutagenesis and biochemical data. Rather, it binds to a distinct, conserved site on the opposite end of Sra1. PMID: 28949297 The NKD1/Rac1 feedback loop regulates the invasion and migration ability of hepatocellular carcinoma cells. PMID: 27231134 the pathological role of Rac1 signaling PMID: 27442895 Study demonstrate that the IAV NS1 protein can directly interact with the cellular protein Rac1 and modulate its activity via post-translational modifications to regulate IAV replication. PMID: 27869202 MiR-142 inhibited the migration, invasion and MMP expression of glioma by targeting Rac1. PMID: 28714015 Rac1 inhibition in gastric adenocarcinoma cells blocks EMT and CSC phenotypes, and thus may prevent metastasis and augment chemotherapy. In gastric adenocarcinoma, therapeutic targeting of the Rac1 pathway may prevent or reverse EMT and CSC phenotypes that drive tumor progression, metastasis, and chemotherapy resistance PMID: 28461325 Taken together, our results indicate that integrin beta6 promotes tumor invasiveness in a Rac1-dependent manner and is a potential biomarker for tumor metastasis. PMID: 27440504 Taken together, these results indicate that DOCK1 is a critical regulator of the malignant phenotypes induced by Rac1(P29S), and suggest that targeting DOCK1 might be an effective approach to treat cancers associated with Rac1(P29S) mutation. PMID: 29432733 Downregulation of PLEKHA7 in PACG may affect BAB integrity and aqueous humor outflow via its Rac1/Cdc42 GAP activity, thereby contributing to disease etiology. PMID: 29016860 MBQ-167 is 10x more potent than other currently available Rac/Cdc42 inhibitors and has the potential to be developed as an anticancer drug, as well as a dual inhibitory probe for the study of Rac and Cdc42 PMID: 28450422 Filamin C promotes lymphatic invasion and lymphatic metastasis and increases cell motility by regulating Rac1/cdc42 activites in esophageal squamous cell carcinoma. PMID: 28031525 We observed Bonferroni-corrected statistically significant interactions between albuminuria, urine cadmium levels and polymorphisms in gene SLC30A7 and RAC1. PMID: 28558300 TIPE2 suppressed tumor invasiveness and angiogenesis in non-small cell lung cancer via inhibiting the activation of Rac1 and subsequently weakening its downstream effects, including F-actin polymerization and VEGF expression. PMID: 27556698 High RAC1 expression is associated with increased cell migration in breast neoplasms. PMID: 27048259 role of Cdc42 and Rac1 activities in pheochromocytoma, the adrenal medulla tumor PMID: 27355516 High RAC1 expression is associated with breast cancer. PMID: 26910843 analysis of the Kindlin-2-RhoGDIalpha-Rac1 signaling axis that is critical for regulation of podocyte structure and function in vivo PMID: 28775002 Ubc9 is an essential regulator of ADAP where it is required for activation of the small GTPase Rac1 in T cell adhesion. PMID: 29127148 MIIP is a key molecule in directing Rac1 signaling cascades in Endometrial carcinoma . Ectopically expressed MIIP consistently competed with Rac1-GTP for binding with the PAK1 p21-binding domain. PMID: 27760566 the Ser179Glu mutant of SDC-4 binds strongly Tiam1, a Rac1-guanine nucleotide exchange factors reducing Rac1-GTP by 3-fold in MCF-7 breast adenocarcinoma cells. PMID: 29121646 ROCK activation phosphorylated Rac1b at Ser71 and increased reactive oxygen species (ROS) levels by facilitating the interaction between Rac1b and cytochrome c. Conversely, ROCK inactivation abolished their interaction, concomitant with ROS reduction. PMID: 28317242 The inhibition of Rac1 by NSC23766 inhibited NADPH oxidase activity and ROS generation induced by high glucose concentrations in INS-1 & human 1.1b4 beta cells. Inhibition of Rac1-NOX complex activation by NSC23766 significantly reduced CD36 expression in INS-1 and human 1.1b4 beta cell membrane fractions. PMID: 27912197 Show More Hide All
Subcellular Location	Cell membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Cytoplasm. Cell projection, lamellipodium. Cell projection, dendrite. Cell junction, synapse.
Protein Families	Small GTPase superfamily, Rho family
Tissue Specificity	Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
Database Links	HGNC: 9801 OMIM: 602048 KEGG: hsa:5879 UniGene: Hs.413812

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Code	CSB-YP019242HU
Size	Pls inquire
Source	Yeast
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Code	CSB-EP019242HU
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Source	E.coli
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Code	CSB-EP019242HU-B
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP019242HU
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Source	Baculovirus
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Code	CSB-MP019242HU
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Source	Mammalian cell
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Recombinant Human Ras-related C3 botulinum toxin substrate 1(RAC1)

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Target Background

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