Recombinant Human Receptor-type tyrosine-protein phosphatase C(PTPRC),partial

Code CSB-YP019049HU
Size US$1298
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names PTPRC
Uniprot No. P08575
Research Area Immunology
Alternative Names B220; CD 45; CD45; CD45 antigen; CD45R; GP180; L-CA; LCA; Leukocyte common antigen; loc; Ly-5; LY5; Ly5, homolog of; Lyt-4; OTTHUMP00000033813; OTTHUMP00000033816; OTTHUMP00000033817; OTTHUMP00000038574; Protein tyrosine phosphatase receptor type c polypeptide; Protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; Protein tyrosine phosphatase, receptor type, c polypeptide; Ptprc; PTPRC_HUMAN; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen
Species Homo sapiens (Human)
Source Yeast
Expression Region 24-575aa
Target Protein Sequence QSPTPSPTGLTTAKMPSVPLSSDPLPTHTTAFSPASTFERENDFSETTTSLSPDNTSTQVSPDSLDNASAFNTTGVSSVQTPHLPTHADSQTPSAGTDTQTFSGSAANAKLNPTPGSNAISDVPGERSTASTFPTDPVSPLTTTLSLAHHSSAALPARTSNTTITANTSDAYLNASETTTLSPSGSAVISTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSFHNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYHLEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHSTSYNSK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 62.8kDa
Protein Length Extracellular Domain
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
Gene References into Functions
  1. Overall, this study reveals the heterogeneity of CD4 effector memory T cells expressing CD45RA and provides insights into T-cell responses against dengue virus and other viral pathogens. PMID: 29133794
  2. Five-year disease-free survival for patients with a high transcriptional expression of CD45 (n = 107) was 62.4% and for patients with a low expression (n = 53) it was 36.2% (P = 0.003). Patients with a high expression of CD45 had a better local recurrence-free survival and disease-specific survival. PMID: 29177949
  3. CD45 is a regulator of IL-2 synergy in the NKG2D-mediated activation of immature human NK cells PMID: 28655861
  4. TCR phosphorylation negatively correlates with TCR-CD45 separation. PMID: 29467364
  5. Our results demonstrate that the expression of CD163 and CD206 in monocytes is modulated by LPS in vitro; LPS induces CD163 expression and downregulates the spontaneously increased expression of CD206 PMID: 25914252
  6. Our results show that the kinetic strategy used in this study may be a simple and easy approach to assess by RT-FCM the reaction between NO and superoxide ion in whole blood monocytes..The no-wash, no-lyse staining protocol with CD45-KO and CD14-PB allows to differentiate clearly and to gate in the monocyte population in near-physiological conditions PMID: 25758468
  7. regulatory effect of the mannose receptor (MR) was mediated by a direct interaction with CD45 on the T cell, inhibiting its phosphatase activity, which resulted in up-regulation of CTLA-4 and the induction of T-cell tolerance. Inhibition of CD45 prevented expression of B-cell lymphoma 6 (Bcl-6), a transcriptional inhibitor that directly bound the CTLA-4 promoter and regulated its activity PMID: 27601670
  8. pUL11 induces IL-10 producing T cells as a result of pUL11 binding to the CD45 phosphatase on T cells. PMID: 28628650
  9. Expression of IL10R subunits within the leukocyte population (CD45(+) cells) was significantly higher in primary brain tumors than in metastases. PMID: 28982901
  10. As CD45 expression vs. SSc is routinely measured in the diagnostics of acute leukemias. PMID: 26415521
  11. A phosphosite within the SH2 Domain of Lck regulates its activation by CD45. A negative feedback loop that responds to signaling events tunes active Lck amounts and TCR sensitivity. PMID: 28735895
  12. C77G is not associated with ovarian cancer in the Norwegian population. However, it is possibly associated with a less aggressive cancer type. PMID: 28759630
  13. Our findings suggest that CD45 is a key regulator of BCR-signaling thresholds mediated by T-cell help PMID: 27056269
  14. we demonstrate for the first time the physiological existence of ct-CD45 in human plasma and show that it may be an extrinsic factor contributing to the maintenance of human T-cell quiescence. PMID: 27718235
  15. Our findings suggest that if w/h ratio on SSC versus CD45 plot is less than 1.6, AML may be considered, and if it is more than 1.6, ALL may be diagnosed. Using morphometric analysis of the blast cluster on SSC versus CD45, it was possible to distinguish between ALL and AML, and their subtypes. PMID: 27748273
  16. Use of the common leukocyte marker CD45 increases the sensitivity of the diagnosis of lymphocytic myocarditis. PMID: 28025077
  17. Review/Meta-analysis: Rheumatoid arthritis patients with PTPRC rs10919563 A allele show a poor response to anti-TNF therapy. PMID: 27074847
  18. Data suggest that CD41 and CD45 expression marked the onset of haemangioblastoma (HB) neovascularisation and the stepwise development of the angioformative period, and also the underlying therapeutic targets of anti-vascular treatment. PMID: 26468019
  19. PTPRC has become the most replicated genetic biomarker of response to TNF inhibitors PMID: 25896535
  20. CD45RO in tumor-infiltrating lymphocytes was found to be a positive prognostic factor in squamous non-small cell lung cancer. PMID: 26678911
  21. CD45RO+ memory T-cells produce IL-17 in patients with atherosclerosis. PMID: 26667768
  22. Data show the structural basis for, and the potent signaling effects of, local CD45 antigen and kinase segregation. PMID: 26998761
  23. a CD45+/CD19 - cell population in bone marrow aspirates correlated with the clinical outcome of patients with mantle cell lymphoma. PMID: 25739938
  24. High CD45 expression is associated with multiple myeloma. PMID: 26994849
  25. C77G T(reg) showed diminished upregulation of activation markers, lower phosphorylation of p56(lck)(Y505) and a reduced proliferative potential when stimulated with anti-TcR or anti-TcR plus CD28 mAb suggesting decreased responsiveness to activating stimuli. Data suggest that the changes in CD45 isoform combination resulting from the C77G mutation alter the responsiveness PMID: 26355564
  26. Findings indicate that CD45 antigen(+) and c-Kit protein(+) hematopoietic cells were more abundant in muscle than in bone marrow between embryonic 14.5 and 17.5 days. PMID: 26389592
  27. CD4+CD45RO+CD25-/lowCD127+: CD4+CD45RO+CD25hiCD127-/low ratio in peripheral blood indicates heart transplant recipients at risk for cardiac allograft vasculopathy. PMID: 25539460
  28. CD45+ cells were abundant in the stroma of physiologically immature placental villi and decreased as pregnancy progressed. PMID: 25043745
  29. This study demonstrated that the copy number variations of PTPRC relate to opioid dependence. PMID: 25345593
  30. This study did not replicate the association between PTPRC and the response to anti-TNF treatment in our Southern European population. We found that TRAF1/C5 risk RA variants potentially influence anti-TNF treatment response. PMID: 25834819
  31. long noncoding RNA encoded by the natural antisense gene of CD45 contributes to the expressional regulation of the CD45RO splicing variant via recruitment of DNA methyltransferase and histone modification modulators specific to the sense gene CD45 PMID: 25381328
  32. In T-cells, cholesterol-dependent domains function in the regulation of the Src family kinase Lck (p56lck) by sequestering Lck from its activator CD45. (Review) PMID: 25658353
  33. Patients with the presence of CD8- and CD45RO-positive T cells in bone marrow demonstrated better survival of gastric cancer patients than those with the absence of these cells in bone marrow. PMID: 25804232
  34. Low expression of CD39(+) /CD45RA(+) on regulatory T cells (Treg ) cells in type 1 diabetic children in contrast to high expression of CD101(+) /CD129(+) on Treg cells in children with coeliac disease. PMID: 25421756
  35. Late-outgrowth CD45 negative endothelial progenitor cells express markers associated with pluripotency and can directly express an osteogenic phenotype under bone differentiation conditions. PMID: 25531767
  36. SLAMF7-triggered inhibition is mediated by a mechanism involving Src kinases, CD45, and SHIP-1 that is defective in MM cells PMID: 25312647
  37. Phosphatase CD45 both positively and negatively regulates T cell receptor phosphorylation in reconstituted membrane protein clusters, depending on LCK activity. PMID: 25128530
  38. Results show that CD45 PTP activity is inhibited by galectin-1 in anaplastic large cell lymphoma cell line H-ALCL. PMID: 24589677
  39. Expressing CD45 promoters containing these regions and tethered to green fluorescent protein (GFP) in a primary B-cell differentiation assay and a transplantation model resulted in high levels of GFP in lymphoid, myeloid, and nucleated erythroid cells PMID: 24852660
  40. The rare sub-population of CD45(-)/Lin(-)/SSEA-4(+) VSEL stem cells survived after Hespan sedimentation. PMID: 24364909
  41. Hematopoietic cell marker CD45 is expressed in hepatic progenitor cells. PMID: 24396288
  42. CD45RA-Foxp3high Tregs increase in the peripheral circulation of head and neck squamous cell carcinoma patients. PMID: 24761979
  43. the regulation of alternative splicing in CD45 by IkappaBL was independent from the kinase activity of CLK1. PMID: 23953137
  44. high CD45 surface expression is associated with a poor prognosis in BCP-ALL as well as in T-ALL. PMID: 23911702
  45. PTPRC/CD45 is down-regulated in leukemogenic tyrosine kinase expressing cells. PMID: 23997015
  46. [review] Circulating CD34+/KDR+/CD45dim endothelial progenitor cells have great potential as biomarkers of vasculogenesis and endothelial repair wjen research protocols of in vitro culture and flow cytometry are included. PMID: 23171577
  47. Heterogeneity within the Lin(-)CD45(-) cell fraction is the likely explanation for differences in the hUCB cell populations. PMID: 23840798
  48. Galectin-3-induced apoptosis of Jurkat cells is regulated by both O-glycans and N-glycans on CD45. PMID: 24211831
  49. spatial regulation of Lck by CD45 and GM1 ganglioside determines the outcome of apoptotic response to Gal-1 and this local regulation may occur only upon intimate effector (Gal-1 expressing) cell-T-cell attachment. PMID: 24231767
  50. A glycosylation-dependent CD45RB epitope defines previously unacknowledged CD27-(IgM high) B cell subpopulations enriched in young children and after hematopoietic stem cell transplantation. PMID: 24211716
  51. The CCR7+CD45RA-CD27+CD28+ central memory subset is significantly decreased in the peripheral blood CD4+ T cells from rheumatoid arthritis patients. PMID: 23747721
  52. Very low levels of surface CD45 reflect chronic lymphocytic leukemia cell fragility, are inversely correlated with trisomy 12, and are associated with increased treatment-free survival. PMID: 23733486
  53. This study analysis revealed up-regulation of CD14, CD18, CD45, TLR2, TLR4, CD74, C1q, C3, CCL2, and down-regulation of CD36, CX3CR1, CX3CL1 and CD200 in postmenopausal women. PMID: 23206327
  54. The E613R allele of CD45 does not function as a hyper- or hypomorphic allele but rather alters the substrate specificity of CD45 for Lyn. PMID: 23396948
  55. hnRNP L represses CD45 exon 4 by recruiting hnRNP A1 to a sequence upstream of the 5' splice site PMID: 23394998
  56. Data indicate a role for cell-surface gal-3 and CD45 in diffuse large B-cell lymphoma (DLBCL) survival and suggest therapeutic targets to sensitize DLBCL cells to death. PMID: 23065155
  57. There is no genetic association between PTPRC exon A 77C>G transversion and liver transplant rejection in a Caucasian population. PMID: 22524693
  58. Continuous culture of CD8+CCR7+ cells results in a population that is homogenously positive for CCR7 and CD45RA. PMID: 22821963
  59. CD100, CD72 and CD45 were expressed in placenta and exhibited different mRNA and protein levels in normal pregnancy and miscarriage. Protein levels were highly dysregulated around 10 weeks of gestation in first and second miscarriage placentas. PMID: 22606231
  60. Patient SNP array analysis revealed no change in copy number but loss of heterozygosity for entire length of chromosome 1, indicating that disease was caused by uniparental disomy with isodisomy of the entire maternal Chr1 bearing the mutant CD45 allele PMID: 22689986
  61. We found increased expression levels of CD40 and CD45 levels in healthy older versus younger individuals. PMID: 22783574
  62. A tumor suppressor role for CD45 in T-cell acute lymphoblastic leukemia. PMID: 22438252
  63. Presence of the rs10919563 rheumatoid arthritis susceptibility variant at the PTPRC gene locus predicts improved response to anti-TNF biologic therapy. PMID: 21952740
  64. The study demonstrates the involvement of CD45-mediated signaling in RBL-induced PBMC proliferation and Th1/Th2 cytokine secretion through activation of p38MAPK and STAT-5. PMID: 22386990
  65. O-glycosylated CD43 and CD45 molecules on T cells regulates cell adhesion and favors the transmission of HTLV-1 from cell to cell. PMID: 22171268
  66. pUL11 has immunosuppressive properties, and that disruption of T cell function via inhibition of CD45 is a previously unknown immunomodulatory strategy of CMV PMID: 22174689
  67. The aim was to determine if SPARC, FOXP3, CD8 and CD45RO expression levels are associated with colorectal cancer (CRC) stage, disease outcome and long-term cancer-specific survival. PMID: 21818290
  68. Data show that CD45+CD19- MCL-ICs play a role in the drug resistance of MCL, and this drug resistance was largely due to quiescent properties with enriched ABC transporters. PMID: 21599592
  69. Data show that Pic, a class 2 SPATE protein produced by Shigella flexneri 2a targets a broad range of human leukocyte glycoproteins including CD43, CD44, CD45, CD93, CD162 and the surface-attached chemokine fractalkine. PMID: 21768350
  70. O-linked glycosylation of CD45RB can be used to follow B cell differentiation and that this regulation may be involved in fine-tuning antigen signaling in the cell. PMID: 21278234
  71. A disease-associated polymorphism alters splicing of the human CD45 phosphatase gene by disrupting combinatorial repression by heterogeneous nuclear ribonucleoproteins (hnRNPs). PMID: 21507955
  72. atRA and TGF-beta, but not IL-2, enable CD4+CD25+CD45RA+ cells to express a phenotype and trafficking receptors similar to natural Tregs PMID: 21179414
  73. The number of CD45-positive cells and mast cells gradually increased with age. PMID: 21106704
  74. the density of CD45RO+ TILs is an independent prognostic factor in non-metastasized (stage I-IIA) Barrett's cancer patients and indicates an important role for the adaptive immunologic microenvironment PMID: 21054833
  75. GSK3/TRAP150, complex regulates CD45 alternative splicing and demonstrate a paradigm for signal transduction from the cell surface to the RNA processing machinery through the multifunctional protein PSF. PMID: 20932480
  76. CD45O immunoexpression in early rheumatoid arthritis synovium. PMID: 20191119
  77. Shift to high molecular weight CD45 isoforms in RNA-binding protein heterogeneous nuclear ribonucleoprotein L (Hnrpll) transgenic thymocytes does not demonstrably affect T cell signaling and selection in vivo. PMID: 20505149
  78. cardiac atrium contains a small C-kit+ CD45- population expressing markers commonly found on endothelial progenitor cells PMID: 20119835
  79. Statistically significant associations were observed between the response to anti-TNF therapy and an rheumatoid arthrits risk allele at the PTPRC gene locus. PMID: 20309874
  80. Expression of protein isoforms correlates with differential proliferative responses of peripheral CD4+ and CD8+ T cells PMID: 20093141
  81. CD45RA-positive and CD45RO-positive regulatory T cells have very distinct gene expression patterns and may not be simply the same cells at different stages of differentiation. PMID: 20231690
  82. CD45 lateral mobility is regulated by the spectrin-ankyrin cytoskeleton of T cells PMID: 20164196
  83. the expression of CD45RA and CD45RO antigens in leukemias PMID: 15624328
  84. CD45 is overexpressed in patients with Crohn's disease. PMID: 19246294
  85. Our data did not reveal any association between CD45 77C>G transversion and susceptibility to Hashimoto's thyroiditis in a German population PMID: 19879912
  86. Aberrant transformation from CD45R0(+)T to CD45RA(+)T has been found in the development of thymocytes of the patients with myasthenia gravis. PMID: 18992193
  87. role of CD45RO in the persistent HTLV-1 infection in vivo PMID: 11778694
  88. CD45 variant (C77G, exon 4) does not confer susceptibility to either IDDM or Graves' disease PMID: 11841494
  89. we examined the frequency of the C77G allele in African and Asian populations from countries with high or low prevalence of HIV infection; Here we report that the variant CD45 C77G allele is absent in African populations PMID: 11862398
  90. Requirements of src family kinase activity associated with CD45 for myeloma cell proliferation by interleukin-6 PMID: 11877294
  91. SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription. PMID: 11909961
  92. analysis of CD45 isoform expression during T-cell development and selection in the human thymus PMID: 11975983
  93. CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells PMID: 11994288
  94. Receptor tyrosine phosphatase, CD45 binds galectin-1 but does not mediate its apoptotic signal in Jurkat cells PMID: 12008046
  95. Role of high and low molecular weight isoforms of CD45 in the function of naive and memory T lymphocytes. Review. PMID: 12022705
  96. a mutation in PTPRC interferes with splicing and alters the structure of the CD45 molecule PMID: 12073144
  97. Biological functions of membrane expressed CD45 isoforms on PMNs were studied. Cross-linking of CD45 isoforms by specific MAbs stimulated different PMN activities by differential suppression on protein tyrosine phosphorylation and p56lck. PMID: 12100025
  98. Role of the C-->G mutation in position 77 of exon 4 of the protein tyrosine phosphatase receptor-type C (PTPRC) gene, coding for the CD45 molecule, for the development of multiple sclerosis (MS) in Italy. PMID: 12147336
  99. The spectrin-ankyrin skeleton controls CD45 surface display and interleukin-2 production. PMID: 12354383
  100. reaches the cell surface via Golgi-dependent and -independent pathways PMID: 12386161

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Involvement in disease Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID); Multiple sclerosis (MS)
Subcellular Location Membrane, Single-pass type I membrane protein, Membrane raft
Protein Families Protein-tyrosine phosphatase family, Receptor class 1/6 subfamily
Database Links

HGNC: 9666

OMIM: 126200

KEGG: hsa:5788

STRING: 9606.ENSP00000356346

UniGene: Hs.654514

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