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Recombinant Human herpesvirus 8 type P E3 ubiquitin-protein ligase MIR2 (K5), partial

Recombinant Human herpesvirus 8 type P E3 ubiquitin-protein ligase MIR2 (K5), partial

Code	CSB-YP310858HKE
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP310858HKE
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP310858HKE-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP310858HKE
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP310858HKE
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Uniprot No.

P90489

Alternative Names

K5E3 ubiquitin-protein ligase MIR2; EC 2.3.2.27; IE1A protein; Modulator of immune recognition 2; ORF K5; RING-type E3 ubiquitin transferase MIR2

Species

Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

E3 ubiquitin-protein ligase which promotes ubiquitination and subsequent degradation of host MHC-I, CD86, ICAM1 and CD1D molecules, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell. Binds target molecules through transmembrane interaction. E3 ubiquitin-protein ligases accept ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfer it to target protein. The result of this ubiquitination is the enhancement of the endocytosis of the target chain and the delivery to the lysosome, where it is proteolytically destroyed. Specific for HLA-A and HLA-B2 alleles.

Gene References into Functions

This work ascribes a critical function to PLP2 for viral ligase activity and underlines the power of non-lethal haploid genetic screens in human cells to identify the genes involved in pathogen manipulation of the host immune system. PMID: 24278019
Both K3 and K5 preferentially associate with DC-SIGN and DC-SIGNR, mediating their ubiquitylation and degradation. PMID: 23460925
Phe119 and Ser120 in the MIR2 ITM region and Asp244 in the B7-2 JM region contribute to the recognition of B7-2 by MIR2. PMID: 22379101
We provide evidence that the KSHV K5 protein is able to drive alterations in cellular signaling cascades, potentially contributing to KSHV oncogenesis. PMID: 21490960
Palmitoylation act to regulate MIR2 function and localization during viral infection. PMID: 21159884
Data show that the polyubiquitin chain generated by the viral E3 ubiquitin ligase, MIR2, is a Lys(11) and Lys(63) mixed-linkage chain, and this ubiquitin chain can function as an internalization signal for MHC I. PMID: 20833710
K5-mediated inhibition of the capture of effector memory CD4-positive T cells can be explained by ubiquitin-dependent downregulation of ICAM-1 expression. PMID: 20357254
K5 is a mechanistically distinct viral countermeasure to tetherin-mediated restriction PMID: 20419159
these results suggest that the effects of MIR2 are gene dosage dependent and that low levels of this viral protein contribute to the widespread downregulation of immune-modulating cell surface proteins during the initial stages of KSHV infection PMID: 17329329
while K5 can act as an E3 ubiquitin ligase to directly mediate cell surface molecule destruction, regulation of its targets occurs through multiple pathways, including ubiquitin-independent mechanisms PMID: 17409151
K5-mediated ubiquitylation signals internalization but not degradation of MICA and causes a potent reduction in NK cell-mediated cytotoxicity. PMID: 18230726
KSHV release is decreased in the absence of K5 in a BST2-dependent manner, suggesting that K5 contributes to the evasion of intracellular antiviral defense programs PMID: 19605472

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Subcellular Location

Host cell membrane; Multi-pass membrane protein. Host endoplasmic reticulum. Note=Probably exerts its effects at the plasma membrane during viral infection.

Database Links

KEGG: vg:4961442

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