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CCNB1 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA026248A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: A431 whole cell lysate(20µg), Hela whole cell lysate(20µg),PC-3 whole cell lysate(20µg)
    All lanes: Cyclin B1 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 48 kDa
    Observed band size: 60 kDa
    Exposure time：1min
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Product Details

Uniprot No.
P14635
Target Names
CCNB1
Alternative Names
CCNB 1 antibody; CCNB antibody; ccnb1 antibody; CCNB1_HUMAN antibody; Cyclin B1 antibody; G2 mitotic specific cyclin B1 antibody; G2/mitotic-specific cyclin-B1 antibody
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human CCNB1
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
14B6
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Cyclin B1, encoded by the CCNB1 gene, serves as a master regulator of the G2/M transition in the cell cycle. As an essential activating partner of CDK1, Cyclin B1 accumulation triggers mitotic entry, making it a critical marker for studying cell proliferation, checkpoint control, and cancer biology. Its dysregulation has been implicated in numerous malignancies, positioning it as both a research target and potential therapeutic marker.

This recombinant monoclonal antibody, generated from clone 14B6 in rabbit host, offers the reproducibility and consistency that demanding cell cycle research requires. Because the antibody sequence is defined and produced recombinantly, you can expect uniform performance across experiments and between lots, eliminating the variability that can complicate longitudinal studies or multi-site collaborations. Affinity chromatography purification ensures high specificity for your target.

Validation in western blot applications demonstrates reliable detection of Cyclin B1 in human cell lysates, including A431, HeLa, and PC-3 lines at dilutions ranging from 1:500 to 1:2000. The observed band at approximately 60 kDa runs slightly higher than the predicted 48 kDa molecular weight, a shift commonly attributed to post-translational modifications such as phosphorylation, which is particularly relevant given Cyclin B1's heavily regulated nature during cell cycle progression.

Whether you are investigating mitotic checkpoint mechanisms, characterizing cell cycle profiles in cancer models, or exploring therapeutic targets affecting proliferation, this antibody provides a dependable tool for your western blot and ELISA workflows. Its validated performance in commonly used human cancer cell lines makes it well-suited for oncology research and cell biology applications where consistent, sequence-defined reagents are essential.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Customer Reviews and Q&A

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Target Background

Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Gene References into Functions
  1. FOXM1 promotes proliferation in human hepatocellular carcinoma cells by transcriptional activation of CCNB1. PMID: 29705704
  2. SNAP23 suppressed progression of cervical cancer and induced cell cycle G2/M arrest via upregulating p21(cip1) and downregulating CyclinB1 PMID: 29908998
  3. Defective cyclin B1 induction by T-DM1 mediates acquired resistance in HER2-positive breast cancer cells. PMID: 28821558
  4. Up-regulation of CCNB1 could be an indicator for invasiveness of pituitary adenomas.Up-regulation of CCNB1 could be an indicator for invasiveness of pituitary adenomas. PMID: 28601573
  5. We identified the known APC/C regulator Cdh1 and the F-box protein Fbxl15 as specific modulators of N-cyclin B1-luciferase steady-state levels and turnover. Collectively, our studies suggest that analyzing the steady-state levels of luciferase fusion proteins in parallel facilitates identification of specific regulators of protein turnover. PMID: 28296622
  6. Overexpression of cyclin B1 is correlated with poor survival in most solid tumors, which suggests that the expression status of cyclin B1 is a significant prognostic parameter in solid tumors. [review] PMID: 27903976
  7. CDK9, in addition to CDK1, has roles in mediating the growth inhibitory effect of dinaciclib on cyclin B1 in triple negative breast cancer PMID: 27486754
  8. Data show that Islet-1 (ISL1) activated the expression of cyclin B1 (CCNB1), cyclin B2 (CCNB2) and c-myc (c-MYC) genes by binding to the conserved binding sites on their promoters or enhancers. PMID: 27183908
  9. XIAP is stable during mitotic arrest, but its function is controlled through phosphorylation by the mitotic kinase CDK1-cyclin-B1 at S40. PMID: 27927753
  10. ZIC5 is highly upregulated in non-small cell lung cancer tumor tissues and may act as an oncogene by influencing CCNB1 and CDK1 complex expression PMID: 27663664
  11. Knockdown of DRG2 elicited down-regulation of the major mitotic promoting factor, the cyclin B1/Cdk1 complex. PMID: 27669826
  12. Mitochondrial Ribosomal Protein L10 regulates cyclin B1/Cdk1 (cyclin-dependent kinase 1) activity and mitochondrial protein synthesis in mammalian cells PMID: 27726420
  13. Changes in expression and localization of cyclin B1 may constitute a part of the mechanism responsible for resistance of HL-60 cells to etoposide. PMID: 27297620
  14. Data suggest that long non-coding RNA ZFAS1 may function as oncogene via destabilization of tumor suppressor protein p53 (p53) and through cyclin-dependent kinase 1 (CDK1)/cyclin B1 complex leading to cell cycle progression and inhibition of apoptosis. PMID: 26506418
  15. exposing renal carcinoma cells to amygdalin inhibited cell cycle progression and tumor cell growth by impairing cdk1 and cyclin B expression PMID: 26709398
  16. Sodium butyrate accelerates 3' UTR-dependent cyclin B1 decay by enhancing the binding of tristetraprolin to the 3' untranslated region of cyclin B1. PMID: 26555753
  17. CDK1-Cyclin B1 activates RNMT, coordinating mRNA cap methylation with G1 phase transcription. PMID: 26942677
  18. Our results demonstrate for the first time that the SYSADOA diacerein decreased the viability of human chondrosarcoma cells and induces G2/M cell cycle arrest by CDK1/cyclin B1 down-regulation. PMID: 26555773
  19. CCNB1 is target of miRNA-410 since its overexpression reduces CCNB1 at protein and mRNA levels. PMID: 26125663
  20. Germ cell tumors consistently overexpressed cyclin B1 independently of their responsiveness to chemotherapy or the presence of p53 mutations. Cyclin B1 was overexpressed by GCT cell lines carrying functional p53. PMID: 25982682
  21. The authors postulate that the mechanism of cytomegalovirus pUL97-human cyclin B1 interaction is determined by an active pUL97 kinase domain. PMID: 26270673
  22. Stereospecific phosphorylation of only the Ser-trans-Pro peptide by Cdk1-cyclin B1 PMID: 25603287
  23. Pharmacological inhibition or siRNA-mediated knockdown of cdk1/CCNB1 induced proliferation arrest independent of MYCN status in neuroblastoma cells. PMID: 26029996
  24. Expression of CDK1 Tyr15, pCDK1 Thr161, Cyclin B1 (total) and pCyclin B1 Ser126 in vulvar squamous cell carcinoma and their relations with clinicopatological features and prognosis. PMID: 25849598
  25. BUB1B expression was highly correlated to CDC20 and CCNB1 expression in multiple myeloma cells, leading to increased cell proliferation. PMID: 25698537
  26. Proximity ligation assay demonstrate proximity between S100A4 and cyclin B1 in vitro, while confocal microscopy showed S100A4 to localize to areas corresponding to centrosomes in mitotic cells prior to chromosome segregation. PMID: 26349943
  27. Cyclin B1 could suppress the invasion and metastasis of colorectal cancer cells through regulating E-cadherin expression, which enables the development of potential intervention strategies for colorectal cancer. PMID: 25962181
  28. CCNB1 is a biomarker for the prognosis of ER+ breast cancer and monitoring of hormone therapy efficacy PMID: 25044212
  29. that CCNB1 contained many CD4 T cell epitopes, which are differentially recognized by pre-existing naive and memory CD4 T cells. PMID: 26136431
  30. CCNB1 activation is associated with recurrence in non-muscle-invasive bladder cancer. PMID: 24714775
  31. PKCa and Cyclin B1 are linked in a DAG-dependent mechanism that regulates cell cycle progression PMID: 25362646
  32. Complete removal of cyclin B1 is essential to prevent the return of the spindle checkpoint following sister chromatid disjunction. PMID: 25483188
  33. Data show that inappropriate overexpression of cyclin B1 causes non-specific cell death. PMID: 25415322
  34. Cyclin B1 marks the restriction point for permanent cell cycle exit in G2 phase. PMID: 25486360
  35. CCNB1 is activated by Chk1, exerts its oncogenic role in colorectal cancer cells, and may play a key role in the development of a novel therapeutic approach against colorectal cancer. PMID: 24971465
  36. Stable association of Cdk1-cyclin B1 with phosphorylated separase counteracts this tendency and stabilizes separase in an inhibited yet activatable state PMID: 25659430
  37. the results were indicative that cyclin B may hold independent prognostic significance, but further studies are required to assess this. PMID: 25315186
  38. that MAD2B may play an important role in high glucose-mediated podocyte injury of diabetic nephropathy via modulation of Cdh1, cyclin B1, and Skp2 expression PMID: 25651564
  39. Expression of cycle marker cyclin B1 differs between benign and malignant papillary breast lesions. PMID: 25501285
  40. Phosphorylated Akt plays an important role in regulating the expression level of cyclin B1 by interacting with AR and increasing the transcriptional activity of AR. PMID: 24574517
  41. Cyclin B1 expression was studied immunohistochemically in specimens from 241 patients with pancreatic cancer and was correlated with clinicopathological features and patient survival. PMID: 25106528
  42. Cyclin B1 overexpression is associated with medullary thyroid carcinoma. PMID: 24488334
  43. study indicates that genetic polymorphisms of CCNB1 and CDK1 are related to BC susceptibility, progression, and survival in Chinese Han women. PMID: 24386390
  44. Parvovirus-induced depletion of cyclin B1 prevents mitotic entry of infected cells. PMID: 24415942
  45. our data suggest that the non-canonical Hh pathway mediated through ptch1 and cyclin B1 is involved in the pathogenesis of NBCCS-associated KCOTs. PMID: 24840883
  46. Allele-dependent transcriptional regulation of CCNB1 associated with the SNPs rs350099, rs350104, and rs164390 affects ISR risk through differential recruitment of NF-Y, AP-1, and SP1. PMID: 24395923
  47. Cyclin B1 and cyclin B2 are interchangeable for ability to promote G2 and M transition in HeLa cells. PMID: 24324638
  48. Our results indicated that CDB(cyclin B destruction box )-fused fluorescent protein can be used to examine the slight gene regulations in the reporter gene system PMID: 24416725
  49. The cyclin B 3'UTR was not sufficient to enhance cyclin B synthesis. PMID: 24058555
  50. Cyclin B1/Cdk1-mediated phosphorylation of mitochondrial substrates allows cells to sense and respond to increased energy demand for G2/M transition and, subsequently, to upregulate mitochondrial respiration for successful cell-cycle progression. PMID: 24746669

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Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Protein Families
Cyclin family, Cyclin AB subfamily
Database Links

HGNC: 1579

OMIM: 123836

KEGG: hsa:891

STRING: 9606.ENSP00000256442

UniGene: Hs.23960

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p53 signaling pathway Cell cycle Cellular senescence FoxO signaling pathway Target Therapy
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