Code | CSB-RA569311A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IF | 1:20-1:200 |
The CXCR4 recombinant monoclonal antibody is produced using recombinant DNA technology and is used for the detection of human CXCR4 protein in ELISA and IF applications. The production process begins by sequencing the cDNA of the CXCR4 antibody-producing hybridomas and synthesizing the gene that codes for the CXCR4 monoclonal antibody. The hybridomas are formed by fusing myeloma cells with B cells isolated from an animal that was immunized with a synthesized peptide derived from human CXCR4. The synthesized gene is then cloned into a vector and transfected into cells for cultivation. The resulting CXCR4 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography.
The CXCR4 protein is primarily expressed on the surface of cells, including immune cells, stem cells, and cancer cells. When CXCL12 binds to CXCR4, it triggers a signaling cascade inside the cell that can lead to a variety of cellular responses that can promote the migration of cells toward areas where CXCL12 is present, such as sites of inflammation or injury. In addition to its role in cell migration, CXCR4 is also involved in cell survival, proliferation, and differentiation. CXCR4 signaling has been implicated in the development of various diseases, including cancer, HIV/AIDS, and inflammatory disorders.
Applications : Immunohistochemistry (IHC)
Sample type: cell
Review: Representative photomicrographs for immunohistochemical staining of CXCR4. Invasive ductal carcinoma showing moderate [A] and strong [B] cytoplasmic and membranous staining of CXCR4. Brown color indicates positive staining. Negative, weak, moderate and strong staining = 0, 1, 2 and 3, respectively. Magnification: ×400, scale bar: 20 μm.
By Anonymous