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| Code | CSB-MP006254HU(F1) |
| Size | How to order? |
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| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
This recombinant human CXCR4 virus-like particle (VLP) is a nanodimensional structure built from viral structural constituents in the form of recombinant proteins synthesized in mammalian cells. Its expression region encodes the full-length human CXCR4 protein (1-352aa). It carries a 10xHis-tag at the C-terminus. Its endotoxin level is less than 1.0 EU/ug measured by the LAL method. It has been validated to be biologically active. In the functional ELISA, this recombinant human CXCR4 VLP can bind to the anti-CXCR4 recombinant antibody, with the EC50 of 101.7-253.6 ng/mL. CXCR4 is a pleiotropic chemokine receptor that regulates a variety of physiological processes through its ligand CXCL12, including immune response, hematopoiesis, developmental processes, and pro-tumorigenic effects. It is almost expressed in all types of hematopoietic cells. The CXCR4 axis stimulates EGFR, MAPK, PI3K/AKT, Wnt/β-catenin, and NF-κB related pathways, which are involved in tumor development, survival, invasion, and homing. CXCR4/CXCL12 signaling is also involved in metastasis. Currently, CXCR4 has been detected in 23 different types of tumors, which is the most commonly expressed chemokine receptor in tumor cells and is associated with the prognosis of patients. The development of CXCR4 antagonists and the down-regulation of CXCR4 expression in cancer cells is of great significance for the treatment of related diseases. |
| Endotoxin | Less than 1.0 EU/ug as determined by LAL method. |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized Human CXCR4 at 10 μg/mL can bind Anti-CXCR4 recombinant antibody (CSB-RA006254MA01HU), the EC50 is 101.7-253.6 ng/mL. |
| Target Names | CXCR4 |
| Uniprot No. | P61073 |
| Alternative Names | (CXC-R4)(CXCR-4)(FB22)(Fusin)(HM89)(LCR1)(Leukocyte-derived seven transmembrane domain receptor)(LESTR)(Lipopolysaccharide-associated protein 3)(LAP-3)(LPS-associated protein 3)(NPYRL)(Stromal cell-derived factor 1 receptor)(SDF-1 receptor)(CD antigen CD184) |
| Molecular Characterization | .svg) |
| Species | Homo sapiens (Human) |
| Source | Mammalian cell |
| Expression Region | 1-352aa |
| Target Protein Sequence | MEGISIYTSDNYTEEMGSGDYDSMKEPCFREENANFNKIFLPTIYSIIFLTGIVGNGLVILVMGYQKKLRSMTDKYRLHLSVADLLFVITLPFWAVDAVANWYFGNFLCKAVHVIYTVNLYSSVLILAFISLDRYLAIVHATNSQRPRKLLAEKVVYVGVWIPALLLTIPDFIFANVSEADDRYICDRFYPNDLWVVVFQFQHIMVGLILPGIVILSCYCIIISKLSHSKGHQKRKALKTTVILILAFFACWLPYYIGISIDSFILLEIIKQGCEFENTVHKWISITEALAFFHCCLNPILYAFLGAKFKTSAQHALTSVSRGSSLKILSKGKRGGHSSVSTESESSSFHSS |
| Mol. Weight | 41.5 kDa |
| Protein Length | Full Length |
| Tag Info |
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions) |
| Form |
Liquid Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged. |
| Buffer | PBS, pH 7.4, 6% trehalose |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Involved in the AKT signaling cascade. Plays a role in regulation of cell migration, e.g. during wound healing. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival.; (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) for human immunodeficiency virus-1/HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.
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| Gene References into Functions |
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| Involvement in disease | WHIM syndrome (WHIMS) |
| Subcellular Location | Cell membrane; Multi-pass membrane protein. Cell junction. Early endosome. Late endosome. Lysosome. Note=In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation cluster (SMAC). |
| Protein Families | G-protein coupled receptor 1 family |
| Tissue Specificity | Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microv |
| Database Links |
HGNC: 2561 OMIM: 162643 KEGG: hsa:7852 STRING: 9606.ENSP00000386884 UniGene: Hs.593413 |
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