FOXO3 Antibody

Datasheet
Code CSB-RA008836A0HU
Size US$350
Image
  • Western Blot
    Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate
    All lanes: FOXO3A antibody at 1.8μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 72, 49 KDa
    Observed band size: 72-90 KDa

  • IHC image of CSB-RA008836A0HU diluted at 1:180 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-RA008836A0HU diluted at 1:180 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of PC3 cells with CSB-RA008836A0HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

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Product Details

Uniprot No. O43524
Target Names FOXO3
Alternative Names AF6q21 antibody; AF6q21 protein antibody; DKFZp781A0677 antibody; FKHR2 antibody; FKHRL 1 antibody; FKHRL1 antibody; FKHRL1P2 antibody; Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody; Forkhead box O3 antibody; Forkhead box O3A antibody; Forkhead box protein O3 antibody; Forkhead box protein O3A antibody; Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody; Forkhead homolog (rhabdomyosarcoma) like 1 antibody; Forkhead in rhabdomyosarcoma like 1 antibody; Forkhead in rhabdomyosarcoma-like 1 antibody; FOX O3A antibody; FOXO2 antibody; foxo3 antibody; FOXO3_HUMAN antibody; FOXO3A antibody; MGC12739 antibody; MGC31925 antibody
Species Reactivity Human
Immunogen A synthesized peptide derived from human FOXO3
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
IF 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC
Gene References into Functions
  1. role in autophagy activation and the maintenance of intracellular homeostasis in inflamed odontoblasts PMID: 29551204
  2. Low FOXO3A expression is associated with colorectal cancer. PMID: 30066886
  3. FoxO3a was overexpressed in 64.71% cases of hepatocellular carcinoma (HCC). FoxO3a overexpression was associated with aggressive phenotypes of HCC, such as histologic grade, stage, and small vessel invasion. FoxO3a overexpression was also correlated with poor disease-free survival. Downregulation of FoxO3a in a HepG2 cell line inhibited cell proliferation and migration. PMID: 29365018
  4. stable knockdown of FOXO3, NCOA3, and TCF7L2 restored growth in low glucose but reduced MEK/MAPK phosphorylation, reduced anchorage-independent growth, and modulated expressions of GLUT1 and Ras pathway related proteins. PMID: 29301589
  5. the inhibition of miR-9 could induce apoptosis in cervical cancer by targeting FOXO3. PMID: 29602130
  6. Study in three European populations present experimental evidence for a functional link between common intronic variants in FOXO3 and human longevity. PMID: 29234056
  7. circRNA-FOXO3 expression was decreased in NSCLC cells and tissue samples. It can inhibit the development of NSCLC cells as a ceRNA through sponging miR-155 and releasing FOXO3 level. PMID: 29620202
  8. The protein expression levels of several autophagy makers, such as LC3I, LC3II and Beclin-1, were higher in FOXO3 plasmid-transfected AGS cells cultured in an acidic microenvironment than in control cells, while P62 protein expression levels were clearly decreased in FOXO3 plasmid-transfected cells compared with control cells. PMID: 30138933
  9. Study suggests that miR-487a-3p might repress CTLA4 and FOXO3 by binding to their 3'UTRs and contribute to the development of T1D. PMID: 29859273
  10. Findings determined that the crucial regions corresponding to the SP1 binding sites located between 2,000 and 1,037 bp were essential for FoxO3a transcriptional activity. Furthermore, FoxO3a transcription was upregulated in response to hypoxic and oxidative stress in colorectal tumor cells (CRC), indicating that the interaction between SP1 and FoxO3a may have important implications in CRC progression. PMID: 29565456
  11. FOXO3a expression correlated with adverse clinicopathological features, such as lymph node metastasis, perineural invasion and higher Ki-67 proliferation index in triple-negative breast cancers. PMID: 29588373
  12. human FOXO3B locus encodes a bona fide human gene; unlike FOXO3A, FOXO3B is cytosolically localized in both the presence and absence of active Akt PMID: 29925039
  13. FoxO3a overexpression increased the transcription and protein expression of Bcl2like protein 11 and cyclindependent kinase inhibitor 1B, and inhibited cyclin D1 transcription and expression. PMID: 29257235
  14. miR-132 negatively regulates palmitate induced NLRP3 inflammasome activation through FOXO3 down-regulation in THP-1 cells. PMID: 29258239
  15. these data ascertain the existence of an H2O2-sensitive PRDX1-FOXO3 signaling axis that fine tunes FOXO3 activity toward the transcription of gene targets in response to oxidative stress. PMID: 28398822
  16. results suggested that SIRT1 deficiency in Bladder cancer cells could suppress cell viability by activating antioxidant response and inducing cell cycle arrest possibly via FOXO3a-related pathways. PMID: 29147649
  17. these results suggest that miR-30b plays important roles in kynurenine-induced increase of FOXO3 expression PMID: 28905195
  18. Authors found that miR-629 negatively regulated FOXO3 protein expression and decreased the activity of a luciferase reporter construct containing the FOXO3 3'-untranslated region. These results show that miR-629 regulates FOXO3 at the posttranscriptional level, resulting in enhanced cell proliferation and invasion of pancreatic carcinoma. PMID: 29072689
  19. auranofin could regulate the Her2/Akt/FOXO3 signaling pathway in SKOV3 cells and be used as a potential antitumor agent considering the expression of MUC4 in ovarian cancer patients. PMID: 28765909
  20. FNDC5 gene interactions with candidate genes FOXOA3 and APOE PMID: 29143599
  21. Results indicate a mechanism for beta-arrestin1 in the regulation of the prostate cancer procession through inhibiting FOXO3a. PMID: 29676828
  22. The findings of this study indicated that FoxO3a knockdown conferred neuroprotective effects after TBI through inhibiting the activation of neuronal autophagy. PMID: 28889023
  23. Low FOXO3A expression is associated with cancer. PMID: 29533771
  24. Data show that forkhead box O3 protein (FOXO3) silencing could inhibit mitophagy and mitochondrial dysfunction induced by manganese chloride (MnCl2). PMID: 28661534
  25. Report an inverse relationship of age with human serum FOXO3A and SIRT3 levels. PMID: 28526626
  26. FOXO3 longevity interactome on chromosome 6 has been described. PMID: 28722347
  27. miR-223-3p regulated cell chemo-sensitivity by targeting FOXO3 in prostatic cancer (PCa) both in vitro and in vivo, providing new potential therapeutic strategy for PCa treatment. PMID: 29518547
  28. H cordata promotes the activation of HIF-1A-FOXO3 and MEF2A pathways. PMID: 27698266
  29. A lower FOXO3 mRNA expression in granulosa cells leads to poor oocyte development in patients with unexplained infertility undergoing controlled ovarian stimulation for in vitro fertilization-embryo transfer. PMID: 28621049
  30. Negative expression of FoxO3/FoxO4 and lymph node metastasis were the risk factors for the poor prognosis of bladder cancer. PMID: 28554751
  31. miR-155-5p promotes fibroblast cell proliferation and inhibits FOXO signaling pathway by negative modulation of both FOXO3 and CDKN1B in vulvar lichen sclerosis PMID: 29339071
  32. Cytoplasmic retention of FOXO3a may represent a potential biomarker for response to combined treatment with inhibitors of PI3K and autophagy in PIK3CA-mutant cervical cancer cells. PMID: 28036259
  33. Results show that FOXO3-phosphorylation at threonine-32 (T32) and nuclear localization in neuroblastoma biopsies significantly correlated with stage IV disease. Data suggest that, depending on the mode and intensity of activation, cellular FOXO3 acts as a homeostasis regulator promoting tumor growth at hypoxic conditions and tumor angiogenesis in high-stage neuroblastoma. PMID: 27769056
  34. FoxO3a might be a key regulator in cetuximab resistance through up-regulating c-Myc in colorectal cancer targeted therapy. PMID: 27825133
  35. Atorvastatin strengthens Skp2 binding to FOXO1 or ICAM1, leading to ubiquitination and degradation. Skp2-dependent ubiquitination of major pathogenic molecules is the key mechanism for statin's protective effect on endothelial function in diabetes. PMID: 28802579
  36. Transcriptional factor PAX3 (PAX3) exerted its tumor suppressor function by inhibiting the activity of major signaling pathways and enhancing expression and activity of transcription factor forkhead box O3 protein (FOXO3a). PMID: 27458157
  37. The result suggests that FOXO3 rs12212067 polymorphism does not play an important role in susceptibility to T. cruzi infection and/or chronic Chagas cardiomyopathy. PMID: 27125259
  38. Overexpression of circ-Foxo3 decreased the interaction between Foxo3 and MDM2, and repressed the function of MDM2 in modulating poly-ubiquitination of Foxo3. PMID: 27886165
  39. silencing FOXO3 diminishes bepridil- and trifluoperazine-induced apoptosis in triple-negative breast cancer cells PMID: 27283899
  40. The authors found that transient TUBB3 activation, through ABCB1, in response to the stimulation of FOXO3a expression, significantly contributes to the cross-resistance of the paclitaxel-resistant cell population and consequently limits the efficacy of both agents where cancer cells have developed multiple resistance. PMID: 27284014
  41. findings provide further evidence for the involvement of FoxO3 during terminal erythropoiesis and confirm the modulation of the PI3K/AKT pathway as a potential therapeutic strategy for beta-thalassemia PMID: 29099866
  42. The data reveal a previously unexplored function of FOXO3a in gastric cancer invasion by regulating proteins involved in extracellular matrix degradation and Epithelial-Mesenchymal Transition. FOXO3a may be of prognostic value and a potential therapeutic target in blocking tumor metastasis. PMID: 27127880
  43. pro-apoptotic role of miR-34a in PA-induced cholangiocyte lipoapoptosis in culture and in the liver PMID: 28250026
  44. Ergosterol peroxide stimulated Foxo3 activity by inhibiting pAKT and c-Myc and activating pro-apoptotic protein Puma and Bax to induce HepG2 cancer cell death. PMID: 27058618
  45. These results show that significantly increased levels of FOXO3, IRF4, and xIAP mRNA in Chinese HIV-1-infected patients. PMID: 27841661
  46. Knockdown of MIEF2 reduces DOX-induced mitochondrial fission and apoptosis in cardiomyocytes and in vivo. Also, knockdown of MIEF2 protects heart from DOX-induced cardiotoxicity. Our study identifies a novel pathway composed of Foxo3a and MIEF2 that mediates DOX cardiotoxicity. PMID: 28137654
  47. data show that the GSK3B-FOXO3 pathway is activated after partial hepatectomy, and this may be one of the mechanisms that lead to upregulation of hepatic IGF1R after partial hepatectomy. PMID: 28952285
  48. Chromosome 6q deletion correlates with poor prognosis and low relative expression of FOXO3 in chronic lymphocytic leukemia patients. PMID: 28699185
  49. Diabetic Glc also promoted beta-catenin nuclear localization and the formation of a complex with FOXO3a that localized to the promoters of Sod2, p21(cip1), and potentially p27(kip1) PMID: 27411103
  50. first report of the association between rs13217795 and allergic rhinitis, and the first independent verification of the association between rs13217795 and asthma PMID: 29141605
  51. in FOXO3-death-resistant cells no point mutations in the TP53-DBD were found-in these cells FOXO3-TP53 complexes are formed and FOXO3-binding to the BIM-promoter, but not the induction of the detoxifying protein SESN3, were prevented, which in turn increased chemo-protection in this type of high-stage-derived neuroblastoma cells PMID: 28869600
  52. Taken together, these findings showed that TRIP6 plays an important role in promoting HCC cells proliferation and may serve as a novel prognostic biomarker and therapeutic target in HCC. PMID: 29080747
  53. NOTCH1 inhibits activation of ATM by impairing the formation of an ATM-FOXO3a-KAT5 complex. PMID: 27524627
  54. FOXO3 is required to induce autophagy and thereby reduce elevated reactive oxygen species levels. PMID: 27532863
  55. A total of 41 differentially expressed genes, such as SOCS3, VAPA, and COL5A2, are speculated to have roles in the pathogenesis of acute myocardial infarction; 2 transcription factors FOXO3 and MYBL2, and 2 miRNAs hsa-miR-21-5p and hsa-miR-30c-5p may be involved in the regulation of the expression of these differentially expressed genes. PMID: 29049183
  56. Downregulation of PKC induces senescence through the AKT-FoxO3a-ROS-p53-p21(Cip1/WAF1) pathway in HCT116 and HEK293 cells. PMID: 28989024
  57. we demonstrated that REP1 blocked the nuclear trans-localization of FOXO3 through physically interacting with FOXO3, thereby suppressing FOXO3-mediated apoptosis. Importantly, the inhibition of REP1 combined with 5-FU treatment could lead to significant retarded tumor growth in a xenograft tumor model of human cancer cells PMID: 28055019
  58. FoxO3a is an early stress response protein to glucose toxicity in diabetic conditions. PMID: 23805295
  59. In cigarette smoke extract stimulated bronchial epithelial cells, carbocysteine increased cell proliferation, and SIRT1 and FoxO3 nuclear expression, and reduced beta galactosidase and survivin expression. PMID: 27237816
  60. geminin selectively couples the transcription factor forkhead box O3 (FoxO3) to HDAC3, thereby specifically facilitating FoxO3 deacetylation. PMID: 28436938
  61. Data suggest that forkhead box O3A protein (FoxO3a) might be a prognostic biomarker or a potential therapeutic target in glioblastoma. PMID: 28295288
  62. our data demonstrated that FOXO3 is essential for CD44 expression and the properties of cancer stem cells (CSCs). Our data also indicated that patients with high FOXO3 activation signatures had poor prognoses. This evidence suggested that cGMP induction and FOXO3 inhibition could be ideal candidates for pancreatic CSC PMID: 27893718
  63. adipose stem cell extract regulates Abeta-induced mitochondrial apoptosis via regulation of P53/foxo3a pathway PMID: 28046000
  64. Human T2D kidneys exhibited more RPTC apoptosis and lower expression of hnRNP F, SIRTUIN-1, and FOXO3alpha than nondiabetic kidneys. PMID: 28424160
  65. acetylation is permissive for generation of the apoptotic form of FOXO3 and the activity of SIRT1 and particularly SIRT7 regulate this process in vivo, allowing control of monocyte apoptosis in response to LPS. PMID: 27669435
  66. ectopic expression of the Foxo3P, Foxo3 circular RNA and Foxo3 mRNA could all suppress tumor growth and cancer cell proliferation and survival. PMID: 26657152
  67. overall survival and progression-free survival were not found to be affected by FOXO3a expression. Cytoplasmic expression of FOXO3a is frequently found in retinoblastoma and may be involved in pathogenesis PMID: 26574636
  68. In prostate cancer, FOXO3a, and YB-1 play inverse reciprocal roles as a tumor-suppressor gene and oncogene, respectively, regulated by ERK signaling. PMID: 27699813
  69. miR-34a/sirtuin-1/foxo3a might play an important role in genistein reversal of ox-LDL-induced oxidative damage in HUVECs. PMID: 28688900
  70. Results indicate that cordycepin promotes caveolin-1 (CAV1)upregulation to enhance c-jun N-terminal kinase (JNK)/forkhead box O3A protein (Foxo3a) signaling pathway activation, inducing apoptosis in lung cancer cells. PMID: 28099944
  71. endothelial-barrier protective role of VEGF in the long term and the importance of Akt1-FoxO signaling on tight-junction stabilization and prevention of vascular leakage through claudin expression, is reported. PMID: 27113546
  72. Activation and overexpression of FoxO3 in granulosa cells of PCOS [polycystic ovary syndrome] women correlated with higher apoptosis levels in these cells suggesting that FoxO3 may be a candidate for the higher apoptosis in granulosa cells from women with PCOS [polycystic ovary syndrome]. PMID: 26993517
  73. clinical cholangiocarcinoma samples displayed FoxO3-Keap1 down-regulation and Nrf2 hyperactivation PMID: 26857210
  74. rs12212067 (a single-nucleotide polymorphism that increases FOXO3 transcription) was associated with reduced inflammation, both biochemically and clinically, and with lower rheumatoid arthritis activity scores. PMID: 27214848
  75. our findings revealed that the FOXO3 variants we tested in our population of Chinese men and women were associated with longevity in men only. None of these associations passed Bonferroni correction PMID: 27936216
  76. the forkhead box O3 (FOXO3)/liver kinase B1 (LKB1)/AMP-activated protein kinase/peroxisome proliferator-activated receptor-gamma co-activator-1beta (PGC-1beta)/pyruvate dehydrogenase-A1 pathway is essential for CD44 expression and cancer stem cells properties. PMID: 28507102
  77. Shikonin induces apoptosis in some lung cancer cells via activation of FOXO3a/EGR1/SIRT1 signaling, and AKT and p300 negatively regulate this process via Bim upregulation. PMID: 27452907
  78. the accumulation of reactive oxygen species (ROS) in cells expressing JAK2V617F compromises the NHE-1/Bcl-xL deamidation pathway by repressing NHE-1 upregulation in response to DNA damage. hematopoietic stem cells (HSCs), FOXO3A is largely localized within the nuclei despite the presence of JAK2V617F mutation, suggesting that JAK2-FOXO signaling has a different effect on progenitors compared with stem cells. PMID: 26234675
  79. PI3K/AKT/FoxO3a signaling pathway role in melanoma cells PMID: 28358423
  80. High FOXO3a and p38 expression could predict the response to cetuximab in patients with CRC harbouring WT KRAS. PMID: 27685445
  81. Kaplan-Meier survival analysis showed that pancreatic ductal adenocarcinoma patients with negative forkhead box P1 and forkhead box O3a expression survived significantly shorter than patients with positive forkhead box P1 and forkhead box O3a expression (p = 0.000). PMID: 28466777
  82. The aims of this study were to quantify the mortality risk for the longevity-associated genotype and to discover the particular cause(s) of death associated with FOXO3 G allele in older Americans of diverse ancestry. PMID: 27071935
  83. These data suggest that miR-592 may promote the progression and metastasis, in part, by targeting FoxO3A in colorectal cancer PMID: 27167185
  84. This study indicates that CKII can modulate the intracellular reactive oxygen species level via FoxO3a. PMID: 27470586
  85. by modulating hypoxia-inducible factor activity via up-regulation of VHL, FOXO3a (foxo3b) plays an important role in survival in response to hypoxic stress. PMID: 27777301
  86. Glucocorticoids treatment was found to rescue FOXO3a expression both in SLE mice and in SLE patients. PMID: 27481940
  87. Carriage of the longevity-associated G allele of FOXO3 snp rs2802292 was a protective factor against CAD mortality in all three populations. In Japanese and Whites, but not in Blacks, the protective effect of the G allele was little changed in models adjusted for other major risk factors. Population-attributable risk (PAR) models found that the nonprotective TT genotype contributed 15%, 9%, and 3% to CAD mortality risk in PMID: 27694344
  88. No statistically significant association was found between the rs2802292 polymorphism and exceptional longevity in either cohort (either examined in their entirety or in a sex-based analysis). PMID: 27621247
  89. Foxo3 gene expression is regulated by miR-551b in ovarian cancer. PMID: 27743201
  90. Results showed that the glioblastoma cell lines resistant to temozolomide exhibited higher levels of FoxO3a and beta-catenin. Also, findings strongly support that FoxO3a renders glioblastoma cells resistant to temozolomide treatment, at least in part, through the regulation of beta-catenin nuclear accumulation. PMID: 28260024
  91. These data reveal a novel role of FOXO3a-dependent gene regulation in the complex adaptive responses of gliomas towards starvation signals. PMID: 27840901
  92. Abeta enhanced association of FOXO3a with mitochondrial DNA (mtDNA), causing a decrease in the expression of cytochrome c oxidase subunit 1 (COX1) and the activity of COX PMID: 26782277
  93. Abnormal PHLPP, FoxO3a, and RAD51 protein expressions may be involved in the development of high- and low-grade ovarian serous adenocarcinomas, suggesting common molecular pathways. PMID: 28139510
  94. we computationally predict FOXO3 as a regulator of this network, and use multiple publicly available in vitro and in vivo experimental datasets to validate that this astrocyte HD network is downstream of a signaling pathway important in adult neurogenesis (TGFb-FOXO3). PMID: 27390852
  95. miR-1307 contributed to prostate cancer proliferation by targeting FOXO3A. PMID: 28122308
  96. Our findings showed that, among these MMP members, only FoxO3a induced MMP9 expression, and MMP9 overexpression reversed the effect that silencing FoxO3a had on the attenuation of cell invasion. Taken together, these data link FoxO3a to the promotion of metastasis in glioma cells. PMID: 27633065
  97. inhibition of mTORC2 enhances ultraviolet B-induced apoptosis in a FOXO3a-dependent manner. PMID: 27058291
  98. Mechanistically, G-Rg1 promoted the phosphorylation of Akt and FoxO3a and led to the cytoplasmic translocation of FoxO3a, which in turn suppressed FoxO3a-modulated expression of proapoptotic Bim and elevated the ratio of Bcl-2 to Bax. PMID: 27522666
  99. This study proposes a new signaling pathway of miR-155/FoxO3a/ARC leading to renal pyroptosis under ischemia/reperfusion injury conditions. PMID: 28006785
  100. inhibition of FoxO3a by IGF-1 via the PI3K/Akt pathway has an important role in IGF-1 induced proliferation and invasion of uveal melanoma cells. PMID: 27881235

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Involvement in disease A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with KMT2A/MLL1.
Subcellular Location Cytoplasm, cytosol, Nucleus
Tissue Specificity Ubiquitous.
Database Links

HGNC: 3821

OMIM: 602681

KEGG: hsa:2309

STRING: 9606.ENSP00000339527

UniGene: Hs.220950

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