ID1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA253477A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: 293T whole cell lysate(30µg), HepG2 whole cell lysate(30µg), 293 whole cell lysate(30µg), A549 whole cell lysate(30µg), U251 whole cell lysate(30µg), A431 whole cell lysate(30µg),BxPC-3 whole cell lysate(20µg),Hela whole cell lysate(30µg)Mouse Brain tissue lysate(30µg) All lanes: ID1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 17 kDa Observed band size: 22 kDa Exposure time: 30s Immunofluorescence staining of PC-3 cell with CSB-RA253477A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of PC-3 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of Hela cell with CSB-RA253477A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Uniprot No.

P41134

Target Names

ID1

Alternative Names

DNA-binding protein inhibitor ID-1 (Class B basic helix-loop-helix protein 24) (bHLHb24) (Inhibitor of DNA binding 1) (Inhibitor of differentiation 1), ID1, BHLHB24 ID

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human Id1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2C7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ID1, or Inhibitor of DNA Binding 1, functions as a dominant-negative regulator of basic helix-loop-helix transcription factors, playing a critical role in controlling cellular differentiation and proliferation. By preventing DNA binding of transcription factor complexes, ID1 helps maintain cells in a proliferative, undifferentiated state—a mechanism that has drawn significant attention in cancer research, where ID1 overexpression is frequently associated with tumor progression, metastasis, and poor prognosis across multiple malignancies.

This recombinant monoclonal antibody (clone 2C7) offers the reproducibility essential for longitudinal studies and multi-site collaborations. Generated against a synthetic peptide derived from human ID1, the sequence-defined nature of recombinant production ensures consistent epitope recognition across lots, eliminating the variability that can compromise experimental comparisons over time.

Validation data demonstrates robust performance across multiple applications. In western blotting, the antibody reliably detects ID1 in a diverse panel of human cell lines including 293T, HepG2, 293, A549, U251, A431, BxPC-3, and HeLa, as well as mouse brain tissue, confirming cross-species reactivity for human and mouse samples. The observed band at 22 kDa runs slightly higher than the predicted 17 kDa molecular weight, which likely reflects post-translational modifications such as phosphorylation that are known to regulate ID1 stability and function. Immunofluorescence applications have been validated in PC-3 and HeLa cells, enabling subcellular localization studies of this nuclear protein.

Whether investigating ID1's role in stem cell maintenance, epithelial-mesenchymal transition, or therapeutic resistance mechanisms, this antibody provides a dependable tool for epigenetics, signal transduction, and cancer biology research programs.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.

Gene References into Functions

Low ID1 expression is associated with breast cancer. PMID: 30066902
ID1, CTCF and ELK1 may be associated with prostate cancer, and may be potential therapeutic targets for the treatment of this disease. PMID: 29956775
CaMKII can directly phosphorylate Beclin 1 at Ser90 to promote K63-linked ubiquitination of Beclin 1 and activation of autophagy; it also promotes K63-linked ubiquitination of inhibitor of differentiation 1/2 (Id-1/2) by catalyzing phosphorylation of Id proteins and recruiting TRAF-6 PMID: 29079782
ID1 expression was associated with the proliferation, invasion and migration of SACC cells. The observed inhibition of SACC cell growth, invasion and migration following knockdown of ID1 expression in the present study, may have been due to restoration of the balance between oncogenic and tumor-suppressive effects resulting from changes in the expression of downstream genes or associated proteins. PMID: 29039489
The mutual correlation between the expression level of TWIST1 and ID1 might be a critical factor driving the process of the human odontoblasts' differentiation. PMID: 29159326
a loss of CULLIN3 represents a common signaling node for controlling the activity of intracellular WNT and SHH signaling pathways mediated by ID1 PMID: 27477274
Id1 enables lung cancer liver colonization by activating an epithelial mesenchymal transformation program in tumor cells and establishing the pre-metastatic niche. PMID: 28549790
High ID1 expression is associated with breast cancer. PMID: 27546618
ID1 expression impacts the sensitivity of colon cancer cells to 5-FU and may be considered as a potential predictive marker in colorectal carcinoma treatment. PMID: 28510612
Reducing ID1 gene expression reduces metastatic spread of salivary gland neoplasms. PMID: 27087608
High ID-1 expression is associated with colorectal carcinoma. PMID: 27633352
ID1 expression was positively related to drug resistance of EGFR-TKI in non-small cell lung cancer. PMID: 27978873
MVD was determined by immunohistochemistry, and the expressions of mRNA and protein of inhibitor of differentiation-1 (ID1) and vascular endothelial growth factor (VEGF) were detected in gastric cancer. PMID: 27610466
ID1 down-regulation induced parallel changes in the IGF and AKT pathways. The crosstalk of these pathways may enhance malignant phenotypes in salivary gland cancer. PMID: 27466488
Results show that ID1, ID3 and IGJ genes are highly expressed in adult B-ALL and correlate with poor prognosis in Hispanic patients. PMID: 27044543
Our findings define an intricate E2F1-dependent mechanism by which Id1 increases thymidylate synthase and IGF2 expressions to promote cancer chemoresistance. The Id1-E2F1-IGF2 regulatory axis has important implications for cancer prognosis and treatment. PMID: 26475334
Peritoneal VEGF-A expression is regulated by TGF-beta1 through an ID1 pathway in women with endometriosis PMID: 26577912
Id-1, a protein repressed by miR-29b, facilitates TGFbeta1-induced EMT in human ovarian cancer cells and represents a promising therapeutic target for treating ovarian cancer. PMID: 24662327
Simultaneous high expression of ID1 and c-Jun or c-Fos was correlated with poor survival in esophageal squamous cell carcinoma patients. PMID: 26858249
Id1 as an important modulator of molecular events during DPSC commitment and differentiation, which should be considered in dental research on tissue engineering. PMID: 24695670
Therefore, we identified a tumorigenic role of Id-1 in OS and suggested a potential therapeutic target for OS patients. PMID: 26797271
these findings provide in vivo genetic evidence of Id1 functions as an oncogene in breast cancer PMID: 25938540
ID1 overexpression may be associated with higher risk karyotype classification and act as an independent risk factor in young non-M3 acute myeloid leukemia patients. PMID: 26191235
Advanced melanoma patients, relative to healthy controls, express much higher levels of ID1 in myeloid peripheral blood cells. PMID: 25924227
LIF has a role in negatively regulating tumour-suppressor p53 through Stat3/ID1/MDM2 in colorectal cancers PMID: 25323535
Overexpression of ID1 is associated with colorectal neoplasia. PMID: 25623217
Inhibitor of differentiation 4 (ID4) acts as an inhibitor of ID-1, -2 and -3 and promotes basic helix loop helix (bHLH) E47 DNA binding and transcriptional activity. PMID: 25778840
Four more genes (BMP4, BMPR1B, SMAD1 and SMAD4) of the ID1 pathway were investigated and only one (BMPR1B) shows the same down regulation PMID: 26072160
Smad1 as a novel binding protein of KSHV latency-associated nuclear antigen (LANA). LANA interacted with and sustained BMP-activated p-Smad1 in the nucleus and enhanced its loading on the Id promoters. PMID: 25010525
berberine's anti-proliferative and anti-invasive activities could be partially rescued by Id-1 overexpression in HCC models, revealing a novel anti-cancer/anti-invasive mechanism of berberine via Id-1 suppression. PMID: 25496992
NSCLC cells with high Id1 protein expression were vulnerable to the treatment of paclitaxel and cisplatin PMID: 25344919
We conclude that Id1 is potently angiogenic and can be up-regulated in endothelial progenitor cells PMID: 24620998
Id-1 and TGF-beta1 played important roles in the progression of gastric cancer, in which Id-1 might act as a downstream mediator of TGF-beta1 signaling through a regulatory mechanism involving N-cadherin and beta-catenin. PMID: 24861919
Downregulation of ID1 by gene silencing can lead to acceleration of TGF-beta1-induced hESC differentiation into ECs and inhibition of proliferation and migration of ECs. PMID: 25549282
Overexpression of ID1 in two different cell lines induced STMN3 and GSPT1 at the transcriptional level, while depletion of ID1 reduced their expression. PMID: 25028095
Our results suggest that Id1 promotes breast cancer metastasis by the suppression of S100A9 expression PMID: 24948111
This study elucidated the potential mechanism underlying Id1 participation in the progression of prostate cancer PMID: 25031707
Data indicate that grap2 and cyclin D1 interacting protein (GCIP) and inhibitor of of DNA binding/differentiation 1 (Id1) are inversely expressed in non-small cell lung cancer (NSCLC) cell lines and specimens. PMID: 24970809
ERbeta1 inhibits the migration and invasion of breast cancer cells and upregulated E-cadherin expression in a Id1-dependent manner. PMID: 25514034
High Id1b, generated by alternative splicing, maintains cell quiescence and confers self-renewal and cancer stem cell-like properties. PMID: 25449776
Data indicate that the inhibitor of DNA binding 1 (Id1)-IGF-II-IGF-IR-AKT signaling cascade plays an important role in esophageal cancer progression. PMID: 24599933
ID1 expression in pancreatic adenocarcinoma was associated with angiogenesis as measured by microvessel density. PMID: 24480377
Studies demostrated the regulatory role of ID1 in the proliferation and metastasis of colorectal cancer. PMID: 24804700
Results show that Id1 and NF-kappaB regulate the expression of CD133 and BMI-1 in an additive or synergistic manner in oral squamous cell carcinoma. PMID: 24572994
Higher Id1 mRNA expression levels might predict a higher hazard ratio for progression and a shorter disease-free survival in prostate cancer. PMID: 24129125
ID1 is a synthetic sick/lethal gene that interacts with the R175H TP53 mutant. PMID: 24378760
These results indicate that Id1 may down-regulate the ability of PC3 cells to form osteolytic lesions in vivo and the signal pathway needs to be further investigated. PMID: 22684559
Id1 induces mesenchymal-to-epithelial transition and pulmonary metastatic colonization by antagonizing Twist1 activity. PMID: 24332369
The results demonstrate that Id-1B decreases the malignancy of lung and prostate cancer cells and counteracts the protumorigenic role of the classical form of Id-1. PMID: 24295493
Inhibitor of differentiation 1 is a prognostic marker for multicentric Castleman's disease. PMID: 24599583

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Subcellular Location

Cytoplasm. Nucleus.

Database Links

HGNC: 5360

OMIM: 600349

KEGG: hsa:3397

STRING: 9606.ENSP00000365280

UniGene: Hs.504609

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Related pathways

Hippo signaling pathway TGF-beta signaling pathway Signaling pathways regulating pluripotency of stem cells Rap1 signaling pathway

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