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CUSABIO conducted an immunization study using a human MUC16/CA125-derived peptide to stimulate an immune response in a rabbit. Splenocytes were isolated from the immunized rabbit and RNA was extracted. The RNA was reverse transcribed into cDNA, which served as a template for amplifying the MUC16/CA125 antibody gene using degenerate primers. The amplified gene fragments were then cloned into an expression vector and transfected into a host system for antibody production. The MUC16/CA125 recombinant monoclonal antibodies were purified from the cell culture supernatant using affinity chromatography. Validation experiments, including ELISA and IHC applications, confirmed the specific binding specificity and affinity of the recombinant MUC16/CA125 monoclonal antibody towards the human MUC16/CA125 protein.
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