Phospho-STAT5A (Y694) Antibody

Datasheet
Code CSB-RA022814A694phHU
Size US$350
Uniprot No. P42229
Image
  • Western Blot
    Positive WB detected in:HepG2 whole cell lysate(treated with EGF or not)
    All lanes:Phospho-STAT5A antibody at 1.025μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 90 KDa
    Observed band size: 90 KDa

  • IHC image of CSB-RA022814A694phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen A synthesized peptide derived from Human Phospho-STAT5A (Y694)
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200
Relevance Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Signal transducer and activator of transcription 5A, STAT5A, STAT5
Immunogen Species Homo sapiens (Human)
Research Area Signal Transduction
Gene Names STAT5A
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Carries out a dual function
Subcellular Location Cytoplasm, Nucleus
Protein Families Transcription factor STAT family
Database Links

HGNC: 11366

OMIM: 601511

KEGG: hsa:6776

STRING: 9606.ENSP00000341208

UniGene: Hs.437058

Pathway ErbB signaling pathway
Jak-STAT signaling pathway
Necroptosis
Th1 and Th2 cell differentiation
Th17 cell differentiation
AGE-RAGE signaling pathway in diabetic complications

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