Code | CSB-RA779090A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
The RPS6KB1 recombinant monoclonal antibody is produced through a carefully orchestrated process to ensure its exceptional quality and specificity. Initially, B cells are isolated from the spleen of an immunized animal, with the synthesized peptide derived from human S6K1 used as the immunogen. RNA is then extracted from these B cells and converted into cDNA through reverse transcription. Amplification of the RPS6KB1 antibody genes is achieved using specific primers targeting the antibody constant regions, followed by their insertion into an expression vector. The expression vector is introduced into host cells via transfection, enabling the production of the RPS6KB1 recombinant monoclonal antibody. After an appropriate incubation period, the antibody is harvested from the cell culture supernatant and meticulously purified using affinity chromatography, ensuring the acquisition of a highly purified form of the RPS6KB1 recombinant monoclonal antibody suitable for diverse applications. To validate its specificity and functionality in detecting human and mouse RPS6KB1 proteins, the antibody undergoes rigorous characterization assays including ELISA and WB analysis. This comprehensive production process guarantees the development of a reliable and effective RPS6KB1 recombinant monoclonal antibody, serving as a valuable tool in various research associated with RPS6KB1.
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