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| Code | CSB-PA825890 |
| Product Type | Polyclonal Antibody |
| Size | US$297 |
| Uniprot No. | Q13485 |
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| Immunogen | Synthesized peptide derived from internal of Human Smad4. |
| Raised in | Rabbit |
| Species Reactivity | Human,Mouse,Rat |
| Specificity | The antibody detects endogenous levels of total Smad4 protein. |
| Tested Applications | ELISA,WB;WB:1:500-1:3000 |
| Relevance | In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8 By similarity. Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for syngernistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Hahn S.A., Science 271:350-353(1996). Zhang Y., Nature 383:168-172(1996). Moskaluk C.A., Diagn. Mol. Pathol. 6:85-90(1997). |
| Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Clonality | Polyclonal |
| Alias | SMAD 4; Mothers against decapentaplegic homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; hSMAD4 |
| Immunogen Species | Homo sapiens (Human) |
| Protocols | ELISA Protocol Western Blotting(WB) Protocol |
| Target Names | SMAD4 |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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| Function | In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Involvement in disease | Pancreatic cancer (PNCA); Juvenile polyposis syndrome (JPS); Juvenile polyposis/hereditary hemorrhagic telangiectasia syndrome (JP/HHT); Colorectal cancer (CRC); Myhre syndrome (MYHRS) |
| Subcellular Location | Cytoplasm, Nucleus |
| Protein Families | Dwarfin/SMAD family |
| Database Links |
HGNC: 6770 OMIM: 114500 KEGG: hsa:4089 STRING: 9606.ENSP00000341551 UniGene: Hs.75862 |
| Pathway |
Hippo signaling pathway
TGF-beta signaling pathway Wnt signaling pathway Adherens junction Cell cycle Signaling pathways regulating pluripotency of stem cells Th17 cell differentiation AGE-RAGE signaling pathway in diabetic complications Apelin signaling pathway FoxO signaling pathway |
SMAD4 Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA021789GA01HU | SMAD4 Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA619768GA01HU | SMAD4 Antibody | Human | ELISA,WB,IHC |
| CSB-PA009961 | SMAD4 Antibody | Human,Mouse,Rat,Monkey | WB,IHC,IF,ELISA |
| CSB-PA619768LB01HU | SMAD4 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA619768LC01HU | SMAD4 Antibody, FITC conjugated | Human | ELISA |
| CSB-PA619768LD01HU | SMAD4 Antibody, Biotin conjugated | Human | ELISA |
SMAD4 Antibodies for Human
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA619768LA01HU | SMAD4 Antibody | Human | ELISA, WB, IHC, IP |
SMAD4 Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP619768HU CSB-EP619768HU CSB-BP619768HU CSB-MP619768HU |
Recombinant Human Mothers against decapentaplegic homolog 4(SMAD4) | Yeast E.coli Baculovirus Mammalian cell |
SMAD4 ELISA Kit for Homo sapiens (Human)
| Code | Product Name | Sample Types | Sensitivity |
|---|---|---|---|
| CSB-E12749h | Human Mothers against decapentaplegic homolog 4,Smad4 ELISA Kit | serum, plasma, tissue homogenates, cell lysates | 6.25 pg/mL |
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