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Western Blot
Positive WB detected in: 15μg hela whole cell lysate
GAPDH antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 5min
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Western Blot
Positive WB detected in: Hela whole cell lysate at 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg
All lanes: GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 5min
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Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate
All lanes: GAPDH antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 30s
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Western Blot
Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate
All lanes GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 30s
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Western Blot
Positive WB detected in: Rat heart tissue, Rat kidney tissue, Rat skeletal muscle tissue, Rat liver tissue, Rat brain tissue tissue, Rat spleen tissue
All lanes GAPDH antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 1min
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Western Blot
Positive WB detected in: Rabbit heart tissue, Rabbit liver tissue, Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue
All lanes GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 5min
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IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of Hela cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunofluorescence staining of HepG2 cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunoprecipitating GAPDH in Hela whole cell lysate
Lane 1: Mouse control IgG instead of CSB-MA000071M0m in Hela whole cell lysate.
Lane 2: CSB-MA000071M0m (1μl) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)
For western blotting, the blot was detected with CSB-MA000071M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
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Overlay histogram showing Hela cells stained with CSB-MA000071M0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1:200/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Overlay histogram showing Jurkat cells stained with CSB-MA000071M0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1:200/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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1.Exosomes extracted from HEPG2 cells
2. Exosomes extracted from PC-3 cells
3. Exosomes extracted from Hela cells
4. Exosomes extracted from U87 cells
5. Hela cell Lysate
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1.Exosomes extracted from MG63 cells
2. Exosomes extracted from Ntera-2 cells
3. MG63 cell Lysate
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1. Exosomes extracted from Raji cells
2. Exosomes extracted from U251 cells
3. Raji cell Lysate
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