Name: Recombinant Human Claudin-3 (CLDN3)-VLPs (Active)
Brand: CUSABIO
SKU: CSB-MP005505HU

Product Details

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CLDN3 at 10 μg/mL can bind Anti-CLDN3 recombinant antibody (CSB-RA005505MA1HU)， the EC50 is 23.62-34.37 ng/mL.VLPs (CSB-MP3838) is negative control.

Target Names

CLDN3

Uniprot No.

O15551

Alternative Names

(Clostridium perfringens enterotoxin receptor 2)(CPE-R 2; CPE-receptor 2)(Rat ventral prostate.1 protein homolog)(hRVP1)

Molecular Characterization

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

1-220aa

Target Protein Sequence

MSMGLEITGTALAVLGWLGTIVCCALPMWRVSAFIGSNIITSQNIWEGLWMNCVVQSTGQMQCKVYDSLLALPQDLQAARALIVVAILLAAFGLLVALVGAQCTNCVQDDTAKAKITIVAGVLFLLAALLTLVPVSWSANTIIRDFYNPVVPEAQKREMGAGLYVGWAAAALQLLGGALLCCSCPPREKKYTATKVVYSAPRSTGPGASLGTGYDRKDYV

Mol. Weight

24.7 kDa

Protein Length

Full Length

Tag Info

C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)

Form

Lyophilized powder
Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This Recombinant Human Claudin-3 (CLDN3) is a biologically active, full-length research reagent optimized for advancing signal transduction and immunology studies of human CLDN3. Synthesized in a mammalian cell expression system, this full-length protein spans amino acids 1–220aa of human CLDN3, with a molecular weight of 25 kDa and a C-terminal 10xHis-tag (detectable only under denaturing conditions) to support purification and detection workflows. A key advantage of this product is its exceptional quality: it has an endotoxin level of less than 1.0 EU/µg as verified by the LAL method, minimizing experimental interference. Critically, this recombinant CLDN3 protein is presented as virus-like particles (VLPs) in an active conformation, with its biological activity rigorously validated via functional ELISA—immobilized CLDN3 at 10 µg/mL can bind Anti-CLDN3 recombinant antibody (CSB-RA005505MA1HU) with an EC₅₀ of 23.62–34.37 ng/mL, while the VLP control (CSB-MP3838) shows no significant binding. This active, native-like structure makes it far more biologically relevant for functional assays, antibody screening, and vaccine development compared to traditional recombinant proteins. This reagent is optimized for human research, providing scientists with a consistent, high-quality tool to dissect CLDN3-mediated biological processes with enhanced physiological relevance and experimental precision.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Applications : Enzymatic activity in vitro

Review: Claudin-3 (CLDN3) is a member of the Claudin family of tight junction proteins and plays an important role in forming intercellular tight junctions. The abnormal expression of the CLDN3 protein is closely related to the occurrence and development of various malignant tumors, making it one of the hot targets in current research. The Claudin-3 is a four times transmembrane protein. The full-length recombinant human CLDN3 protein expressed by CUSABIO through its VLP platform has good activity. I used the CSB-MP005505HU product for an ELISA experiment, and the EC50 was measured to be 3.157-5.258 ng/ml, which can be used for my subsequent experimental research, and the results have been promising. The protein is stable with good repeatability, so I will continue to purchase in the future.

By Anonymous

Code	CSB-MP005505HU
Abbreviation	Recombinant Human CLDN3 protein-VLPs (Active)
MSDS	MSDS Datasheet
Size	$558
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Image	CSB-MP005505HU is detected by Mouse anti-6*His monoclonal antibody. Activity Measured by its binding ability in a functional ELISA. Immobilized Human CLDN3 at 10μg/mL can bind Anti-CLDN3 recombinant antibody (CSB-RA005505MA1HU)， the EC₅₀ is 23.62-34.37 ng/mL.VLPs (CSB-MP3838) is negative control. Biological Activity Assay
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Recombinant Human Claudin-3 (CLDN3)-VLPs (Active)

Product Details

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