Bone Related Research ELISA Kits

Bone Related ELISA Kits

Bones are the hardest organs within the bodies of humans and vertebrate animals, located either internally or on the surface. Due to their diverse forms, intricate structures, and rich composition, bones serve several biological functions, including protecting vital organs, enabling movement, providing body support and structure, hematopoiesis, mineral and fat storage, endocrine regulation, acid-base and calcium balance regulation [1]. Many diseases can affect the skeletal system, including fractures, arthritis, osteoporosis, bone infections, and tumors.

CUSABIO offers a variety of ELISA kits for important enzymes or protein detection involved in the processes of bone biology. These kits assist in a comprehensive understanding of bone formation, remodeling, metabolism, and other related areas in the field of bone biology, thus supporting your research and development efforts for bone-related disease treatments.

1. Bone formation and bone remodeling

The process of bone formation is called ossification. During the embryonic development stage, bone formation mainly occurs through two mechanisms: intramembranous ossification and endochondral ossification.

Intramembranous ossification primarily occurs in the formation of flat bones, where bone is formed directly from connective tissue, such as mesenchymal tissue. Primitive mesenchymal cells differentiate into osteoblasts, which gradually mature to form bone with all its histological characteristics. Several key factors need to be activated during this process, including bone morphogenetic protein 2 (BMP2), transforming growth factor-beta (TGF-β), runt-related transcription factor 2 (Runx2), among others. Among these factors, bone morphogenetic protein is the only growth factor capable of inducing the transformation of tissue cells into osteoblasts.

On the other hand, endochondral ossification occurs in long bones and most other bones in the body. The main difference between the two mechanisms is that endochondral ossification involves the use of calcified cartilage as a template for osteoblasts, unlike intramembranous ossification.

Related targets: BMP, TGF-β, Runx2, Sp7

● Bone morphogenetic protein ELISA kits
Code Product Name Sensitivity Target
CSB-EL002733MO Mouse Bone morphogenetic protein 1(BMP1) ELISA kit 7.81 pg/mL BMP1
CSB-E04508r Rat Bone morphogenetic protein 2,BMP-2 ELISA Kit 1.95 pg/mL BMP2
CSB-EL002739HU Human Bone morphogenetic protein 3(BMP3) ELISA kit 3.9 pg/mL BMP3
CSB-E04511r Rat Bone morphogenetic protein 4,BMP-4 ELISA Kit 1.95 pg/mL BMP4
CSB-E08122h Human Bone morphogenetic protein 7,BMP-7 ELISA Kit 3.9 pg/mL BMP7
CSB-E08125m Mouse Bone morphogenetic protein 7,BMP-7 ELISA Kit 0.156 pg/mL   BMP7
CSB-EL002746HU Human Bone morphogenetic protein 8B(BMP8B) ELISA kit 2.34 pg/mL BMP8B
CSB-E17529h Human bone morphogenetic protein 15 (BMP-15/GDF-9B)ELISA kit 3.9 pg/mL BMP15
CSB-E04516h Human Bone morphogenetic protein receptor Ⅱ,BMPR-Ⅱ ELISA Kit 1.56 pg/mL BMPR2
CSB-EL009343HU Human Bone morphogenetic protein 3B(GDF10) ELISA kit 5.8 pg/mL GDF10
● Runx2 ELISA kit
Code Product Name Sensitivity Target
CSB-E12935h Human Core Binding Factor alpha1 CBFA1/RUNX2 ELISA Kit 7.8 pg/mL RUNX2

Bone remodeling, as a basic element in skeletal activity, is a process in which old bone tissues are absorbed (osteoclasts) and new bone tissues are formed (osteoblasts) to maintain the stable structure and function of bones. The mutual regulation among osteoblasts, osteoblasts and osteoclasts can maintain the dynamic coupling balance in the process of bone remodeling, in which osteoblasts and osteoclasts play a key role. The purpose of bone remodeling is to regulate calcium homeostasis, repair bone micro-damage caused by daily mechanical pressure, and shape the bone during growth.

2. Bone matrix

Bone matrix is the primary structural component of the skeletal system, composed of a variety of organic and inorganic substances. The organic components are secreted by osteoblasts and consist of 90% collagen, predominantly Type I collagen, and 10% non-collagenous proteins. Non-collagenous proteins include osteocalcin, alkaline phosphatase, osteopontin, cell adhesion proteins, bone growth factors, and more. While that inorganic component are mainly minerals such as calcium salt and phosphate.

Related targets: BGLAP, CALC(CT), BALP

● Osteocalcin and calcitonin ELISA kits
Code Product Name Sensitivity Target
CSB-E13345c Dog Osteocalcin/bone gla protein,OT/BGP ELISA Kit 0.078 ng/mL BGLAP
CSB-E05129r Rat Osteocalcin/Bone gla protein,OT/BGP ELISA kit 31.25 pg/mL BGLAP
CSB-E06917m Mouse Osteocalcin/Bone gla protein,OT/BGP ELISA kit 19.5 pg/ml BGLAP
CSB-E05128h Human Osteocalcin/Bone gla protein,OT/BGP ELISA kit 7.8 pg/ml BGLAP
CSB-E08210h Human calcitonin gene related peptide,CGRP ELISA Kit 0.39 pg/mL BGLAP
CSB-EQ027706MO Mouse calcitonin gene related peptide (CGRP) ELISA kit 3.9 pg/mL CGRP
CSB-E05131h Human Calcitonin,CT ELISA Kit 1.172 pg/mL CALCA
CSB-E16438p Pig calcitonin gene related peptide(CGRP)ELISA Kit 7.81 pg/mL CGRP
CSB-E08211r Rat calcitonin gene related peptide,CGRP ELISA Kit 1.56 pg/mL CGRP
● Alkaline phosphatase ELISA kits
Code Product Name Sensitivity Target
CSB-E09033h Human bone alkaline phosphatase,BALP ELISA Kit 7.8 ng/ml BALP
CSB-E11914m Mouse Bone-specific alkaline phosphatase (BALP) ELISA kit 0.31 ng/mL BALP
CSB-E11865r Rat bone alkaline phosphatase, BALP ELISA Kit 3.9 U/L BALP

3. Synthesis and degradation of collagen

Synthesis: Collagen is a soluble precursor protein. After the synthesis of pro-collagen chains within the cell's ribosomes, they undergo modification and packaging and are then secreted into the extracellular matrix. There, they assemble into mature collagen helices and undergo cross-linking, ultimately forming structurally stable collagen fibers.

Related targets: PICP, HPC III, Col

● Collagen-related ELISA kits
Code Product Name Sensitivity Target
CSB-E08082h Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit 0.39 ng/mL Col Ⅰ
CSB-E08084r Rat Collagen Type Ⅰ,Col Ⅰ ELISA Kit 19.5 pg/mL Col Ⅰ
CSB-E06858Rb Rabbit Collagen Type Ⅰ,Col Ⅰ ELISA Kit 0.039 ng/mL Col Ⅰ
CSB-E08087h Human Collagen Type Ⅱ,Col Ⅱ ELISA Kit 7.8 pg/mL Col Ⅱ
CSB-E07924r Rat Collagen Type Ⅲ,Col Ⅲ ELISA KIT 0.039 ng/mL Col Ⅲ
CSB-E04799h Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT 3.12 ng/mL Col Ⅲ
CSB-E07925m Mouse Collagen Type Ⅲ,Col Ⅲ ELISA KIT 0.78 pg/mL Col Ⅲ
CSB-E17116h Human Collagen Type Ⅳ(COL Ⅳ) ELISA Kit 15.6 pg/mL Col Ⅳ
CSB-EL005715HU Human Collagen alpha-1(X) chain (COL10A1) ELISA kit 0.078 ng/mL COL10A1
CSB-EL005724HU Human Collagen alpha-1(XVII) chain (COL17A1) ELISA kit 0.039 ng/mL COL17A1
CSB-E13445h Human collagen, type I, alpha 1 (COL1A1) ELISA kit 0.156 ng/mL COL1A1
CSB-EL005727MO Mouse Collagen alpha-1(I) chain (COL1A1) ELISA kit 0.078 ng/mL COL1A1
CSB-E12732r Rat Collagen alpha-1(I) chain (COL1A1) ELISA kit 0.039 ng/mL COL1A1
CSB-EL005739HU Human Collagen alpha-1(II) chain (COL2A1) ELISA kit 0.078 ng/mL COL2A1
CSB-E13446h Human Collagen, type III, alpha 1 (COL3A1)ELISA Kit 0.039 ng/mL COL3A1
CSB-EL005741HU Human Collagen alpha-1(IV) chain (COL4A1) ELISA kit 7.81 pg/mL COL4A1
CSB-EL005742HU Human Collagen alpha-2(IV) chain (COL4A2) ELISA kit 11.75 pg/mL COL4A2
CSB-EL005743HU Human Collagen alpha-3(IV) chain (COL4A3) ELISA kit 0.039 ng/mL COL4A3
CSB-E16375h Human Collagen Type Ⅵ(COLⅥ) ELISA Kit 7.81 pg/mL COL6A1
CSB-EQ027853PI Pig collagen type I (COLI) ELISA kit 19.5 pg/mL COLI
CSB-E11989h Human procollagen type III ,HPCⅢ ELISA kit 25 ng/mL HPCⅢ
CSB-E08079h Human Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit 15.6 pg/mL PⅠCP

Degradation: Collagen is cleaved and degraded by a group of matrix metalloproteases (MMPs) as part of the bone remodeling process. This protease can initiate the cleavage of collagen fibers at neutral pH, and is the core of matrix decomposition and bone remodeling.

Related targets: MMP

● Matrix metalloproteinase ELISA kits
Code Product Name Sensitivity Target
CSB-E04672h Human Matrix metalloproteinase 1,MMP-1 ELISA kit 0.078 ng/mL MMP1
CSB-E14057c Canine Matrix metalloproteinase 1,MMP-1 ELISA kit 0.04 ng/mL MMP-1
CSB-E07417m Mouse matrix metalloproteinase 1,MMP-1 ELISA kit 11.75 pg/mL MMP-1
CSB-E04676m Mouse matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit 3.9 pg/mL MMP2
CSB-E07411r Rat matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit 0.156 ng/mL MMP2
CSB-E04675h Human Matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit 0.195 ng/mL MMP2
CSB-E13621c Dog Matrix metalloproteinase 3,MMP-3 ELISA kit 0.39 ng/mL MMP3
CSB-E04678m Mouse matrix metalloproteinase 3,MMP-3 ELISA kit 0.078 ng/ml MMP3
CSB-E07410r Rat matrix metalloproteinase 3,MMP-3 ELISA kit 0.39 pg/mL MMP3
CSB-E07409m Mouse matrix metallopeptidase 7 (matrilysin, uterine) (MMP7) ELISA kit 7.81 pg/mL MMP7
CSB-E04680h Human Matrix metalloproteinase 8/Neutrophil collagenase,MMP-8 ELISA kit 9.77 pg/mL MMP8
CSB-E08008r Rat matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit 0.195 ng/ml MMP9
CSB-E04673h Human Matrix metalloproteinase 10,MMP-10 ELISA kit 19.5 pg/mL MMP10
CSB-EL014659MO Mouse Macrophage metalloelastase(MMP12) ELISA kit 1.95 pg/mL MMP12
CSB-E13208h Human Matrix Metalloproteinase 12(MMP-12) ELISA Kit 0.156 ng/mL MMP12
CSB-E07412r Rat matrix metalloproteinase 13,MMP-13 ELISA kit 23.44 pg/mL MMP13
CSB-E14220Rb Rabbit Matrix Metalloproteinase 13,MMP-13 ELISA Kit 23.44 pg/mL MMP13
CSB-E14055c Canine Matrix metalloproteinase 13,MMP-13 ELISA kit 0.039 ng/mL MMP-13
CSB-E13157h Human Matrix Metalloproteinase 14,MMP-14 ELISA Kit 0.01 ng/ml MMP14

4. Mineralization

Mineralization is the process in which inorganic salts gradually deposit between collagen protein fibers in the bone matrix. This process renders bone tissue hard and durable while maintaining the calcium-phosphorus balance within the body. Mineralization may require alterations in the accumulation of original fibers and the composition of non-collagenous proteins. Proteins such as Osteopontin (OPN), Bone Sialoprotein (BSP), Dentin Matrix Protein 1 (DMP1), Dentin Sialophosphoprotein (DSPP), Matrix Extracellular Phosphoglycoprotein (MEPE), among others, play crucial roles in calcium deposition within the bone matrix. These proteins collectively belong to the SIBLINGs group. They are detected in many tumors and play essential roles in their malignant transformation, invasion, and metastasis processes, indicating that SIBLINGs hold potential as diagnostic and prognostic tools and as novel therapeutic targets [2–4].

Related targets: OPN, BSP, DMP1, DSPP, MEPE

● Related ELISA kits
Code Product Name Sensitivity Target
CSB-E08392h Human Osteopontin, OPN ELISA Kit 0.195 ng/mL OPN
CSB-E12008m Mouse Bone sialoprotein, BSP ELISA kit 0.78 pg/mL BSP

5. Other common bone-related ELISA kits

Code Product Name Sensitivity Target Function
CSB-E08490h Human tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit 0.078 mIU/mL ACP5 Blood indexes of osteoclast activity in human body
CSB-E08492m Mouse tartrate-resistant acid phosphatase 5b(TRACP-5b) ELISA Kit 0.02 mIU/mL
CSB-EL006488HU Human dystroglycan 1 (dystrophin-associated glycoprotein 1) (DAG1) ELISA kit 31.25 pg/mL DAG1 Maintain structural and functional stability between muscle and bone
CSB-E09714h Human Matrix Gla Protein,MGP ELISA Kit 1.95 pg/mL MGP Regulated mineralization
CSB-EL017269MO Mouse Osteocrin(OSTN) ELISA kit 3.9 pg/mL OSTN Affect bone development and metabolism
CSB-E12021h Human osteocrin,Ostn ELISA kit 0.078 ng/mL  
CSB-EL017436HU Human Pappalysin-2(PAPPA2) ELISA kit 0.5 ng/mL PAPPA2 Embryonic development related
CSB-E08762m Mouse osteonectin,ON ELISA Kit 7.81 pg/mL SPARC Inhibit cell adhesion, regulating cell cycle and promote tissue fibrosis
CSB-EL022604HU Human Secreted phosphoprotein 2 (SPP2/SPP24) ELISA kit 3.9 ng/mL SPP2 Participate in bone formation
CSB-E04692h Human Osteoprotegerin,OPG ELISA KIT 1.95 pg/mL TNFRSF11B Osteoprotegerin, inhibiting the activation of osteoclasts
CSB-E04693m Mouse Osteoprotegerin,OPG ELISA KIT 3.9 pg/mL
CSB-E07404r Rat Osteoprotegerin,OPG ELISA KIT 0.195 ng/ml
CSB-E13415h Human Tartrate Resistant Acid Phosphatase(TRAP)ELISA Kit 0.12 ng/mL TRAP Relate to osteoclast function
CSB-E08491r Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit 0.078 mIU/mL
CSB-EC013789HU Human undercarboxylated matrix Gla protein (ucMGP)ELISA kit 0.039 ng/mL ucMGP Regulated mineralization



[2] Chaplet M, et al. Expression of dentin sialophosphoprotein in human prostate cancer and its correlation with tumor aggressiveness. Int J Cancer. 2006;118:850–856.

[3] El-Tanani MK, et al. The regulation and role of osteopontin in malignant transformation and cancer. Cytokine Growth Factor Rev. 2006;17:463–474.

[4] Chen J, et al. Bone sialoprotein promotes tumor cell migration in both in vitro and in vivo models. Connect Tissue Res. 2003;44(Suppl 1):279–284.

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