ENTPD1, also known as ectonucleoside triphosphate diphosphohydrolase 1, is a crucial enzyme with a structure that allows it to bind to magnesium and calcium ions, enabling it to participate in various biochemical processes. This enzyme plays a pivotal role in cell attachment, signal transduction, blood coagulation, and humoral immune responses against microbes, ensuring cellular stability, precise signal transmission, normal blood clotting, and effective immune defense. Although the specific mechanisms of the signaling pathways involving ENTPD1 are still under investigation, it is speculated that it may interact with other signaling molecules or receptors to regulate crucial cellular processes. With further research, we will gain a more comprehensive understanding of the complexity of ENTPD1 and its vital role in the living system.
Validated Data
Positive WB detected in: HL-60 whole cell lysate, HepG2 whole cell lysate, U251 whole cell lysate
All lanes: ENTPD1 antibody at 2.6μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58, 59, 35, 43, 47, 60 kDa
Observed band size: 58, 43 kDa
IHC image of CSB-PA007690LA01HU diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-PA007690LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The following Entpd1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
Entpd1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA007690GA01HU | ENTPD1 Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA006195 | ENTPD1 Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA080017 | ENTPD1 Antibody |
Human | WB, ELISA |
CSB-PA981013 | ENTPD1 Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA007690LA01HU | ENTPD1 Antibody |
Human | ELISA, WB, IHC, IF |
CSB-PA007690LD01HU | ENTPD1 Antibody, Biotin conjugated |
Human | ELISA |
CSB-RA568544A0HU | ENTPD1 Recombinant Monoclonal Antibody |
Human | ELISA, FC |
Entpd1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP007690HU CSB-EP007690HU CSB-BP007690HU CSB-MP007690HU CSB-EP007690HU-B |
Recombinant Human Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
Entpd1 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL007690HU | Human Ectonucleoside triphosphate diphosphohydrolase 1(ENTPD1) ELISA kit |
serum, plasma, tissue homogenates | 5.86 pg/mL |