LAG-3 is an immunoinhibitory transmembrane protein comprised of key components, including four immunoglobulin superfamily domains in the extracellular region, a transmembrane domain, and serine phosphorylation sites in the intracellular domain. Functionally, LAG-3 primarily interacts with ligands such as MHC II and galectin-3 to suppress T cell activation and cytokine secretion, thereby maintaining immune homeostasis. In terms of signaling, LAG-3 engagement with its ligands triggers intracellular interactions with downstream signaling molecules, modulating the activation state of T cells. In cancer immunotherapy, LAG-3 serves as a crucial target, and blocking or inhibiting LAG-3 can potentiate T cell-mediated tumor killing. Therefore, elucidating the structure, function, and signaling pathways of LAG-3 holds significant promise for the development of novel immunotherapy strategies.
Validated Data
Positive WB detected in: HepG2 whole cell lysate
All lanes: LAG3 antibody at 4.9µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58, 40 kDa
Observed band size: 58 kDa
Immunofluorescence staining of HepG2 cells with CSB-PA012719LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The following Lag3 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
Lag3 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA012719GA01HU | LAG3 Antibody |
Human,Mouse | ELISA,WB |
CSB-PA012719LA01HU | LAG3 Antibody |
Human | ELISA, WB, IF |
CSB-PA012719LC01HU | LAG3 Antibody, FITC conjugated |
Human | |
CSB-PA012719LD01HU | LAG3 Antibody, Biotin conjugated |
Human | ELISA |
CSB-PA005868 | LAG3 Antibody |
Human | WB, ELISA |
CSB-PA172061 | LAG3 Antibody |
Human | ELISA,WB |
CSB-MA012719A0m | LAG3 Monoclonal Antibody |
Human | ELISA, WB, IHC, FC |
Lag3 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP012719HU1 CSB-BP012719HU1 CSB-MP012719HU1 CSB-EP012719HU1-B |
Recombinant Human Lymphocyte activation gene 3 protein (LAG3), partial |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-CF012719HU | Recombinant Human Lymphocyte activation gene 3 protein (LAG3) |
in vitro E.coli expression system |
CSB-EP012719HU1 | Recombinant Human Lymphocyte activation gene 3 protein (LAG3), partial |
E.coli |
Lag3 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL012719HU | Human Lymphocyte activation gene 3 protein(LAG3) ELISA kit |
serum, plasma, tissue homogenates | 0.039 ng/mL |