E7 Antibody

Code CSB-PA365855ZA01HML
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Product Details

Full Product Name Rabbit anti-Human papillomavirus type 16 E7 Polyclonal antibody
Uniprot No. P03129
Target Names E7
Alternative Names E7 antibody; Protein E7 antibody
Raised in Rabbit
Species Reactivity Human papillomavirus type 16
Immunogen Recombinant Human papillomavirus type 16 Protein E7 (E7) (1-98AA)
Immunogen Species Human papillomavirus type 16
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Made-to-order

Target Data

Function Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta).
Gene References into Functions
  1. A role for E7 protein in regulation of Langerhans cells homeostasis in the skin and in suppression of antigen specific CD8 T cell expansion. PMID: 27708419
  2. E7 oncogene alters host gene expression in the cervical stroma. PMID: 29073104
  3. direct evidence that both A3A and HPV16 E7 interact with CUL2, suggesting that the E7-CUL2 complex formed during HPV infection may regulate A3A protein levels in the cell. PMID: 29367246
  4. Results suggest that E7 recruited CUL2, driven by CUL2/E2F1/miR-424 regulatory loop, is overexpressed and accelerates HPV16-induced cervical carcinogenesis. PMID: 27153550
  5. The human papillomavirus E7 oncoprotein is a regulator of host transcription. (Review) PMID: 27818212
  6. Findings indicate the interaction network of viral oncogene HPV16 E7, miR-27b and PLK2, and support the potential strategies using antisense nucleic acid of miR-27b for therapy of cervical cancer. PMID: 26910911
  7. Based on experimental data obtained from E7 proteins from the prototypical viral types 16, 18 and 11, we identified a couple of low pKa nucleophilic cysteines that can form a disulfide bridge upon the exposure to ROS and regulate the cytoplasm to nucleus transport PMID: 27863297
  8. E7 is major transforming oncoprotein of human papillomavirus 16 and serves as paradigmatic example of intrinsically disordered proteins due to its N-terminal disordered domain. Data suggest mutation of leucines in N-terminal intrinsically disordered domain of E7 leads to pronounced increase in both alpha-helix and beta-sheet structures; thus, E7 appears to exhibit local structural elements that oppose canonical folding. PMID: 28952717
  9. Authors observed thousands of unique HPV16 genomes; very few women shared the identical HPV16 sequence, however, E7 was devoid of variants in precancers/cancers compared to higher levels in the controls; this was confirmed in cancers from around the world. Strict conservation of the 98 amino acids of E7, which disrupts Rb function, is critical for HPV16 carcinogenesis. PMID: 28886384
  10. study determined that high-risk E7 proteins target the proteolysis of the cellular protein tyrosine phosphatase PTPN14 and find that this activity is correlated with the retinoblastoma-independent transforming activity of E7 PMID: 27651363
  11. Increased phosphorylation of the N29S of E7 increases interaction with TBP and pRb and viral transforming activity. PMID: 27829177
  12. HPV16 E7 oncoprotein upregulates Oct3/4, Sox2, Nanog and Fgf4 expression to maintain the self-renewal capacity of cancer stem cells. PMID: 27693927
  13. result indicates that cervical cancers do not require the continuous expression of E7 on the Fanconi anemia pathway-deficient background PMID: 27190216
  14. the level of Cdc6 phosphorylation at serine 54 (S54P) was increased in E7-expressing cells. S54P was associated with an increase in the total amount of Cdc6 and chromatin-bound Cdc6. DNA damage-enhanced upregulation and chromatin binding of Cdc6 appeared to be due to downregulation of cyclin-dependent kinase 1 (Cdk1) as Cdk1 knockdown increased Cdc6 levels PMID: 27207654
  15. PTPN14 is classified as a potential tumor suppressor protein, and is very susceptible to HPV E7-induced proteasome-mediated degradation. PMID: 28100625
  16. study found that HPV-16 decreased the phosphorylation of AKT (pAKT) and that this is a function of E7 that is independent of the Rb degradation function PMID: 27030265
  17. These data suggest that the HPV16 E7 oncoprotein impairs the function and morphology of dendritic cells and induces the systemic accumulation of CD11b(+)Gr1(+) cells. PMID: 27478837
  18. the structural features of the flexible N-terminal region (46 residues) of E7, were investigated. PMID: 27418281
  19. The human papillomavirus type 16 oncogene E7 may affect STK31 expression through a DNA methylation-mediated mechanism. PMID: 27044426
  20. HPV16 E7 protein can up-regulate host miR-21 expression, thus elevating cervical carcinoma cell growth, proliferation and invasion. PMID: 26884851
  21. These data suggest that local expression of HPV16-E7 in keratinocytes can contribute to persisting infection with this oncogenic virus, by altering the phenotype and function of local APCs. PMID: 27031095
  22. Results found that HPV16 E7 increases RARB mRNA and protein expression both in vitro and in the cervix of young K14E7 transgenic mice suggesting that RARB overexpression is part of the early molecular events induced by the E7 oncoprotein. PMID: 26173416
  23. E7 localised to the plasma membrane in cervical cancer cells. PMID: 26131956
  24. findings suggest that increased IL-17A expression by macrophages in E7-expressing skin exposed to DNCB promotes arginase-1 induction and contributes directly to the observed hyperinflammation. PMID: 25720383
  25. the modulation of HPV-16 E6/E7 expression remarkably influenced cell proliferation, migration, and invasion, as well as the protein levels of E-cadherin and P-cadherin in cervical cell lines. PMID: 26093522
  26. The data showed that E7 induced CCNA1 methylation by forming a complex with Dnmt1 at the CCNA1 promoter, resulting in the subsequent reduction of expression in cancers. PMID: 26250467
  27. In 10 microg/ml BV-treated CaSki cells, the mRNA expression and protein levels of HPV16 E7 were significantly decreased. The mRNA and protein expression levels of HPV16 E7 were decreased by bee venom in TC-1 tumors. PMID: 25633640
  28. that mast cells, recruited towards CCL2 and CCL5 expressed by epithelium induced to proliferate by Human Papillomavirus 16 E7 protein PMID: 25340820
  29. These results point to a mutually functional interaction between p14ARF and E7 that might partly explain why the sustained p14ARF expression observed in most cervical pre-malignant lesions and malignancies may be ineffective. PMID: 24798431
  30. In genetically engineered mouse models expressing HPV16 oncogenes in stratified squamous epithelia, HPV16 E7, alone or together with E6, led to an accumulation of epithelial cells harboring gamma-H2AX nuclear foci. PMID: 25216575
  31. Data indicate that HPV16E7, CBP/p300, and retinoblastoma protein pRb interactions are potentially important for cellular transformation. PMID: 25451029
  32. Our results demonstrate that HPV16.E7 protein enhances drug associated production of arginase-1 by myeloid cells and consequent inflammatory cellular infiltration of skin. PMID: 24732401
  33. The aim of the present study was to investigate the cytotoxicity of natural killer (NK) cells to CaSki cells following knockdown of the E7 protein of the human papillomavirus type 16. PMID: 24566606
  34. Results show that E7 interacts with the B-Myb, FoxM1 and LIN9 components of this activator complex, leading to cooperative transcriptional activation of mitotic genes in primary cells and E7 recruitment to the corresponding promoters. PMID: 24141769
  35. E7 induces cancer by causing DNA damage at least in part through the inactivation of pocket proteins. PMID: 24013229
  36. E7 induces human papillomavirus-associated head and neck cancers by promoting DNA damage through the inactivation of pocket proteins. PMID: 24086435
  37. HPV16 E7 oncoprotein increases production of the IL18BP in keratinocytes. PMID: 24478434
  38. The seven cysteines in HPV16 E7 remain reduced in conditions resembling the basal reduced state of a cell. PMID: 24559112
  39. This study demonstrates that the cdk inhibitor p16INK4A is required for high-level expression of the E7 oncoproteins of HPV-16, HPV-18, and HPV-45 in cervical carcinoma cells. PMID: 24599991
  40. These data suggest that the interaction of human papillomavirus E7 with p190 dysregulates this GTPase activating protein and alters the actin cytoskeleton. PMID: 24403595
  41. Matrix metalloproteinases (MMP)--MMP-1,-2,-9 and its endogenous activity regulators in transformed by E7 oncogene HPV16 and HPV18 cervical carcinoma cell lines PMID: 24479343
  42. E7 proteins of different types of human papillomaviruses disrupted pocket protein-DREAM complexes in a similar extent. PMID: 24294959
  43. hTERT cooperates with HPV16 E7 protein in mediating bypass of the senescence blockade and effecting cell immortalization PMID: 23592995
  44. Human papillomavirus type 16 E7 expression causes increased EMI1 mRNA expression and also inhibits EMI1 degradation. PMID: 24074588
  45. that a patch of hydrophobic residues, 65LRLCV69, within the zinc-binding domain of HPV16 E7 mediates its nuclear import via hydrophobic interactions with the FG domain of the central channel nucleoporin Nup62. PMID: 24074597
  46. HPV16-induced TLR9 down-regulation affects the interferon response which negatively regulates viral infection PMID: 23752229
  47. Authors investigated the effect of E7 on the late promoter and found that E7 was able to activate the promoter. PMID: 23725693
  48. expression of a single oncoprotein in the epidermis is sufficient for lymphocyte trafficking (including immunosuppressive lymphocytes) to premalignant skin PMID: 23469070
  49. Human papillomavirus 16 E7 Adenine647Guanine can be used as a candidate marker for the progression of the cervical neoplasia. PMID: 21535311
  50. These results demonstrate an important role for Cdt1 in human papillomavirus E7-induced rereplication and shed light on mechanisms by which human papillomavirus induces genomic instability. PMID: 23152514

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Subcellular Location Host cytoplasm, Host nucleus
Protein Families Papillomaviridae E7 protein family
Database Links

KEGG: vg:1489079

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