The Human CASP3 ELISA Kit allows for the in vitro quantitative determination of CASP3 concentrations in serum, plasma, cell lysates, or tissue homogenates. This kit exclusively recognizes human CASP3. CASP3 is a frequently activated death protease and is responsible for the proteolytic cleavage of many specific target proteins leading to typical morphological changes in apoptotic cells. Studies have shown that CASP3 induced apoptosis in neurons during programmed developmental cell death and that CASP3 is required for normal brain development. CASP3-induced apoptosis has also been observed in microglia and astrocytes of developing brains. In addition to its apoptotic functions, CASP3 has also been demonstrated to influence the survival, proliferation, and differentiation of both normal and malignant cells and tissues. CASP3 is also necessary for the development of the auditory function.
The detection mechanism of this kit is based on the Sandwich-ELISA technique. CASP3 in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled CASP3 antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with CASP3 content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.