Human Caspase 3,Casp-3 ELISA Kit

Code CSB-E08856h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P42574
Lead Time 3-5 working days
Abbreviation CASP3
Protein Biological Process 1 Apoptosis/Autophagy
Target Name caspase 3, apoptosis-related cysteine peptidase
Alias CPP32, CPP32B, SCA-1, PARP cleavage protease|SREBP cleavage activity 1|Yama|apopain|caspase 3|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3
Species Homo sapiens (Human)
Protein Biological Process 3 Apoptosis
Sample Types serum, plasma, cell lysates, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer s disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
HGNC 1504
RGD 2275
MGI 107739
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-101  
1:2 Average % 94  
Range % 83-101  
1:4 Average % 96  
Range % 90-106  
1:8 Average % 94  
Range % 85-105  
Recovery
The recovery of human Casp-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-104  
EDTA plasma (n=4) 96 91-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.758 2.762 2.760 2.653  
10 2.476 2.461 2.469 2.362  
5 2.002 1.902 1.952 1.845  
2.5 1.245 1.209 1.227 1.120  
1.25 0.622 0.612 0.617 0.510  
0.625 0.308 0.325 0.317 0.210  
0.312 0.212 0.224 0.218 0.111  
0 0.105 0.109 0.107    
References
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Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Database Links

HGNC: 1504

OMIM: 600636

KEGG: hsa:836

STRING: 9606.ENSP00000311032

UniGene: Hs.141125

Pathway MAPK signaling pathway
p53 signaling pathway
TNF signaling pathway
Apoptosis
IL-17 signaling pathway
Natural killer cell mediated cytotoxicity
AGE-RAGE signaling pathway in diabetic complications
ER Stress pathway

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