Human Caspase 3,Casp-3 ELISA Kit

Instructions
Code CSB-E08856h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name caspase 3, apoptosis-related cysteine peptidase
Alternative Names A830040C14Rik ELISA Kit; Apopain ELISA Kit; CASP 3 ELISA Kit; CASP-3 ELISA Kit; CASP3 ELISA Kit; CASP3_HUMAN ELISA Kit; Casp3a ELISA Kit; Caspase 3 ELISA Kit; Caspase 3; apoptosis-related cysteine peptidase ELISA Kit; Caspase 3; apoptosis-related cysteine protease ELISA Kit; Caspase 3; apoptosis-related cysteine protease a ELISA Kit; Caspase-3 subunit p12 ELISA Kit; Caspase3 ELISA Kit; CC3 ELISA Kit; CPP 32 ELISA Kit; CPP-32 ELISA Kit; CPP32 ELISA Kit; CPP32B ELISA Kit; Cysteine protease CPP32 ELISA Kit; EC 3.4.22.56 ELISA Kit; ICE3 ELISA Kit; LICE ELISA Kit; mldy ELISA Kit; OTTHUMP00000165052 ELISA Kit; OTTHUMP00000165053 ELISA Kit; OTTHUMP00000165054 ELISA Kit; PARP cleavage protease ELISA Kit; Procaspase3 ELISA Kit; Protein Yama ELISA Kit; SCA 1 ELISA Kit; SCA-1 ELISA Kit; SCA1 ELISA Kit; SREBP cleavage activity 1 ELISA Kit; Yama ELISA Kit; Yama protein ELISA Kit
Abbreviation CASP3
Uniprot No. P42574
Species Homo sapiens (Human)
Sample Types serum, plasma, cell lysates, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-101  
1:2 Average % 94  
Range % 83-101  
1:4 Average % 96  
Range % 90-106  
1:8 Average % 94  
Range % 85-105  
Recovery
The recovery of human Casp-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-104  
EDTA plasma (n=4) 96 91-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.758 2.762 2.760 2.653  
10 2.476 2.461 2.469 2.362  
5 2.002 1.902 1.952 1.845  
2.5 1.245 1.209 1.227 1.120  
1.25 0.622 0.612 0.617 0.510  
0.625 0.308 0.325 0.317 0.210  
0.312 0.212 0.224 0.218 0.111  
0 0.105 0.109 0.107    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Data

Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Gene References into Functions
  1. The optimal melatonin concentration (3 mM) significantly decreased the intracellular reactive oxygen species levels, caspase-3 activity and the percentage of both dead and apoptotic-like sperm cells and increased the vitality, progressive motility and total motility and AKT phosphorylation compared with the control group. PMID: 29196809
  2. As phosphorylation of the serine residue of this tetra-peptide could yield a motif similar to the caspase-3 binding recognition sequence DEVD/E, the region from a representative PE_PGRS protein (PE_PGRS45) was docked to human caspase-3. PMID: 30207307
  3. within the modified loop, Ser(150) evolved with the apoptotic caspases, whereas Thr(152) is a more recent evolutionary event in mammalian caspase-3. Substitutions at Ser(150) result in a pH-dependent decrease in dimer stability, and localized changes in the modified loop propagate to the active site of the same protomer through a connecting surface helix. PMID: 29414778
  4. Caspase-3 and -8 and annexin V may serve as diagnostic markers in Ovarian cancer , also explained that the decrement in control of the S phase in the cell cycle may considered one of the significant factors in the development of ovarian tumors PMID: 30197345
  5. Study seems to indicate a direct connection between SNPS in CASP3 gene and prostate cancer (PCa) risk in the Galician population after stratification. Also, individual susceptibility to PCa becomes more evident when assessing gene-environment interactions. Alleles G and T, in rs1049216 and rs2705897 respectively, are related to an increased risk of PCa in smokers overweight individuals. PMID: 30176316
  6. Low CASP3 expression is associated with Colorectal Cancer. PMID: 29801534
  7. overexpressed miR-337-3p and miR-17-5p/miR-132-3p/-212-3p can regulate executioner caspases-3 and -7, respectively. PMID: 29659498
  8. Caspase-8 and Caspase-3 expressions in tumor tissues are novel candidate prognostic markers for colorectal cancer patients PMID: 29355114
  9. The new findings of this work were that an association between serum caspase-3 concentrations during the first week, apoptosis degree, sepsis severity, and sepsis mortality exists. PMID: 29119350
  10. our data demonstrate that WT1 protein undergoes proteolytic processing by caspase-3 in chemotherapeutic drugs-induced apoptosis. This processing is associated with a reduction of WT1 protein. PMID: 28395566
  11. Increased baseline gene expressions of RUNX2, p21 and caspase 3 in the peripheral blood might predict better responses to methotrexate therapy. PMID: 28741869
  12. The caspase-3-mediated movement of PUS10 and the release of mitochondrial contents enhancing caspase-3 activity creates a feedback amplification loop for caspase-3 action. Therefore, any defect in the movement or interactions of PUS10 would reduce the TRAIL sensitivity of tumor cells. PMID: 28981101
  13. a prolonged anti-apoptotic intervention targeting caspase-3 should be considered with caution due to the potential adverse effects in mitochondria dynamics resulting from a novel potential functional role of procaspase-3 in mitochondrial biogenesis via regulating the expression of mitochondrial biogenesis activators. PMID: 28585712
  14. knockdown of RPA1 suppressed cell clone formation, induced cell cycle arrest at the G1 phase and promoted cell apoptosis by regulating the protein level of Caspase 3 PMID: 29601890
  15. The phosphorylation level of p38 was upregulated by MA1 treatment, and the inhibitor of p38, SB203580, attenuated the MA1-induced p38 phosphorylation as well as caspase3 and PARP activation. These results indicate that MA1 treatment alters invasive and oncogenic phenotypes of human colorectal cancer cells through the stimulation of the p38 signaling pathway PMID: 28713983
  16. Overexpression of full-length AIFM1 suppresses proliferation and induces apoptosis of HepG2 and Hep3B cells. Caspase 3 and DRAM are involved in full-length AIFM1-induced apoptosis in HepG2 and Hep3B cells. PMID: 29501488
  17. Here the authors show that sublethal activation of Caspase-3 plays an essential, facilitative role in Myc-induced genomic instability and oncogenic transformation. PMID: 28691902
  18. we show that ABT-737 and TQ activate PKA in a caspase-3-dependent manner, which correlates with platelet inhibition and apoptosis and therefore potentially contributes to the bleeding risk in chemotherapy patients PMID: 28661475
  19. MiR-221 might represent a candidate biomarker of likelihood of response to Sorafenib in HCC patients to be tested in future studies. Caspase-3 modulation by miR-221 participates to Sorafenib resistance PMID: 28096271
  20. In the present study, galangin was found to suppress laryngeal cancer cell proliferation. Also, flow cytometry, immunohistochemical and western blot analysis indicated that cell apoptosis was induced for galangin administration, promoting caspase-3 expression through regulating PI3K/AKT/NF-kappaB. PMID: 28677816
  21. 1,4-BQ evidently induced mitochondria-mediated apoptosis and increased pro-apoptotic genes (Caspase-9 and Caspase-3) expression in a dose-dependent manner. PMID: 27425441
  22. GGN played a tumor-promoting role in bladder cancer through regulation of NFkappaB/caspase3-mediated apoptosis signaling. PMID: 29412153
  23. Serum caspase-3 concentrations are increased in ICH patients as well as correlate with clinical severity and prognosis PMID: 28526532
  24. High caspase-3 expression is significantly associated with adverse breast cancer-specific survival. High caspase-3 expression was significantly associated with HER2 positive tumours.The prognostic significance of caspase-3 expression in different breast cancer phenotypes was also examined.There was a significant association in receptor positive (ER, PR or HER2) and non-basal like subgroups. PMID: 27798717
  25. UV phototoxicity-induced pre-elafin inside keratinocytes prior to cornified envelope formation could be involved in UV-induced keratinocyte apoptosis via cystatin-A downregulation resulting in pro-caspase-3 activation. PMID: 28119996
  26. Overexpression of CASP3 is associated with Breast Cancer. PMID: 26932709
  27. Results show that CASP3 expression is regulated by HOXC13 which represses its transcription by directly targeting its promotor region. PMID: 29168599
  28. Data show that selective histone deacetylase 6 (HDAC6) inhibition or knockdown of HDAC6 expression was able to prevent caspase 3 activation in lung endothelial cells and maintain lung endothelial cell-cell junctions. PMID: 27419634
  29. Genetic variations in the CASP3 gene and the joint effects of working time and CASP3 polymorphisms may modify the risk of developing noise-induced hearing loss PMID: 28738811
  30. Data indicate that through upregulating the expression of caspase-3, the TT genotype of caspase-3 rs1049216 can be associated with not only the risk of cervical cancer but also the progression of this cancer. PMID: 28114230
  31. In conclusion, our findings firstly revealed that GSDME switches chemotherapy drug-induced caspase-3 dependent apoptosis into pyroptosis in gastric cancer cells. PMID: 29183726
  32. Everolimus also induced higher levels of caspase-3/-7 activation in GR over GS cells, and everolimus-mediated mTOR inhibition lead to G2 arrest in GR cells but G1 arrest in GS cells. PMID: 28165150
  33. Results Grb7 and Hax1 may colocalize partially to mitochondria in EGF-treated SKBR3 cells and their interaction can affect Caspase3 cleavage of Hax1 supporting an inhibitory role of Grb7 on Casp3 cleavage function by interfering with the association of Casp3 and Hax1. PMID: 26869103
  34. caspase-3 inhibitors also suppressed the attenuation of cell adhesion and phosphorylation of p38 MAPK by EGF-F9. Our data indicated that EGF-F9 activated signals for apoptosis and induced de-adhesion in a caspase-3 dependent manner. PMID: 27129300
  35. Data indicate that E-cadherin and caspase-3 were targets of miR-421, which was up-regulated by HIF-1alpha. PMID: 27016414
  36. findings suggest that caspase-3 activation can trigger necrosis by cleaving GSDME and offer new insights into cancer chemotherapy PMID: 28459430
  37. These results demonstrate that hyperglycemic-induced endothelial microparticles increase endothelial cell active caspase-3. This apoptotic effect may be mediated, at least in part, by a reduction in miR-Let-7a expression. PMID: 28942148
  38. Epigallocatechin-3-Gallate protects against Ang II-induced HUVEC apoptosis by decreasing oxidative stress and ameliorating mitochondrial injury via activation of Nrf2/casp3 signaling pathway. PMID: 28942440
  39. Prolonged treatment of human PMNs or mice bone marrow derived neutrophils (BMDN) with nitric oxide led to enhanced reactive oxygen species generation, caspase-8/caspase-3 cleavage, reduced mitochondrial membrane potential and finally cellular apoptosis. PMID: 27584786
  40. cleaved caspase-3 and caspase-3/8/9 could be biomarkers for tumorigenesis in oral tongue squamous cell carcinoma patients. PMID: 28700659
  41. TT genotype of CASP3 rs4643701 polymorphisms showed risk in CAD. CASP3 rs4647601 creates a new exon splicing enhancer. PMID: 28633917
  42. These findings shed some light on how a tumor cell may avert apoptosis using Hsp60 and point to the anti-cancer potential of drugs, such as CubipyOXA, which interfere with Hsp60/pC3 complex formation, and thus allow the apoptotic cascade to proceed. PMID: 28212901
  43. Here the authors show that in macrophages SipA induces increased caspase-3 activation early in infection. PMID: 28630067
  44. SASH1 is cleaved by caspase-3 following Ultraviolet C-induced apoptosis. PMID: 27831555
  45. Caspase 3 activation in dying glioma cells unfavorably supported post-irradiation angiogenesis. PMID: 27826040
  46. CASP3 is a direct target of specific Epstein-Barr virus BART miRNAs. PMID: 27565721
  47. Data suggest that EV71 infection in enterocytes does not inhibit phosphorylation of STAT1/2 induced by IFN-beta, but p-STAT1/2 transport into the nucleus is significantly blocked; EV71 infection in enterocytes down-regulates expression of KPNA1 and induces degradation of cellular KPNA1 via caspase-3. [EV17 = Enterovirus 71] PMID: 28455446
  48. our results identified that mammalian sterile 20-like kinase 1 is a novel downstream target of pyruvate kinase M2, and knockdown of pyruvate kinase M2 contributes apoptosis via promoting nuclear translocation of mammalian sterile 20-like kinase 1 by enhancing Caspase-3-dependent cleavage. PMID: 28656802
  49. High levels of FADD and caspase-8, but not caspase-3, were associated with increased incidence of coronary events in subjects from the general population. PMID: 28302628
  50. Interestingly, EspC-induced apoptosis was triggered through a dual mechanism involving both independent and dependent functions of its EspC serine protease motif, the direct cleavage of procaspase-3 being dependent on this motif. PMID: 27329750

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Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Database Links

HGNC: 1504

OMIM: 600636

KEGG: hsa:836

STRING: 9606.ENSP00000311032

UniGene: Hs.141125

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