Code | CSB-RA182616A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
The process of producing a CASP3 recombinant antibody involves four steps. First, the CASP3 monoclonal antibody gene is sequenced, followed by incorporating the gene into a plasmid vector. Then, the recombinant vector is introduced into a host cell line, and the CASP3 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. The CASP3 monoclonal antibody is derived from hybridomas that produce the CASP3 antibody, and a synthesized peptide derived from human CASP3 is used as the immunogen during the production of the CASP3 monoclonal antibody. This CASP3 recombinant monoclonal antibody is recommended for use in ELISA, WB, and IHC applications to detect human CASP3 protein.
CASP3 is a cysteine protease enzyme that plays a critical role in the process of programmed cell death, or apoptosis, in cells. When cells undergo apoptosis, caspase-3 is activated by upstream caspases and cleaves a variety of cellular proteins, ultimately leading to the destruction of the cell. CASP3 is responsible for many of the characteristic changes that occur during apoptosis, such as DNA fragmentation, nuclear condensation, and plasma membrane blebbing. It is also involved in the regulation of inflammation and the immune response. In addition to its role in apoptosis, caspase-3 has been implicated in other cellular processes, including cell proliferation and differentiation.
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