Caspase-3 is a protein in humans that is encoded by CASP3 gene. Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
The following CASP3 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CASP3 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004548GA01HU | CASP3 Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA05689A0Rb | CASP3 Antibody |
Human | ELISA, IHC, IF |
CSB-PA05689C0Rb | CASP3 Antibody, FITC conjugated |
Human | |
CSB-PA05689B0Rb | CASP3 Antibody, HRP conjugated |
Human | ELISA |
CSB-PA05689D0Rb | CASP3 Antibody, Biotin conjugated |
Human | ELISA |
CSB-PA240326 | CASP3 Antibody |
Human | ELISA,WB |
CSB-PA148736 | Cleaved-CASP3 (A175) Antibody |
Human, Mouse, Rat | ELISA, WB, IHC |
CSB-PA000003 | Cleaved-CASP3 (D175) Antibody |
Human | WB, IHC, ELISA |
CSB-PA000004 | Cleaved-CASP3 (D175) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA000020 | CASP3 Antibody |
Human,Monkey | WB, ELISA |
CSB-PA000022 | Cleaved-CASP3 (D175) Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA001231 | CASP3 Antibody |
Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-PA005133 | CASP3 Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA060268 | Cleaved-CASP3 (D175) Antibody |
Human | IHC, ELISA |
CSB-PA060269 | Phospho-CASP3 (S150) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-MA080226 | CASP3 Monoclonal Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA080230 | CASP3 Antibody |
Human | WB, IHC |
CSB-PA423088 | CASP3 Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA984107 | CASP3 Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA786000 | CASP3 Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA140280 | CASP3 Antibody |
Human,Mouse,Rat | IHC,ELISA |
CSB-RA286668A0HU | CASP3 Antibody |
Human | ELISA, WB, IHC, IF |
CSB-RA182616A0HU | CASP3 Antibody |
Human | ELISA, WB, IHC |
CSB-RA241798A0HU | CASP3 Antibody |
Human | ELISA, WB, IHC |
CSB-RA833808A0HU | CASP3 Antibody |
Human | ELISA, WB, IHC |
CASP3 Antibodies for Arabidopsis thaliana (Mouse-ear cress)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA163748XA01DOA | CASP3 Antibody |
Arabidopsis thaliana | ELISA, WB (ensure identification of antigen) |
CASP3 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP004548MO CSB-EP004548MO CSB-BP004548MO CSB-MP004548MO CSB-EP004548MO-B |
Recombinant Mouse Caspase-3(Casp3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP3 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP004548HU CSB-BP004548HU CSB-MP004548HU CSB-EP004548HU-B |
Recombinant Human Caspase-3(CASP3),partial |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP004548HU | Recombinant Human Caspase-3(CASP3),partial,Escherichia Coli |
E.coli |
CASP3 Proteins for Xenopus laevis (African clawed frog)
Code | Product Name | Source |
---|---|---|
CSB-YP004548XBE CSB-EP004548XBE CSB-BP004548XBE CSB-MP004548XBE CSB-EP004548XBE-B |
Recombinant Xenopus laevis Caspase-3(casp3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP3 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP004548RA CSB-EP004548RA CSB-BP004548RA CSB-MP004548RA CSB-EP004548RA-B |
Recombinant Rat Caspase-3(Casp3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP3 Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP600954BO CSB-EP600954BO CSB-BP600954BO CSB-MP600954BO CSB-EP600954BO-B |
Recombinant Bovine Caspase-3(CASP3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP3 Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Code | Product Name | Source |
---|---|---|
CSB-YP651605MOV CSB-EP651605MOV CSB-BP651605MOV CSB-MP651605MOV CSB-EP651605MOV-B |
Recombinant Macaca fascicularis Caspase-3(CASP3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP3 Proteins for Pan troglodytes (Chimpanzee)
Code | Product Name | Source |
---|---|---|
CSB-YP705445EQV CSB-EP705445EQV CSB-BP705445EQV CSB-MP705445EQV CSB-EP705445EQV-B |
Recombinant Pan troglodytes Caspase-3(CASP3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP3 Proteins for Saimiri boliviensis boliviensis (Bolivian squirrel monkey)
Code | Product Name | Source |
---|---|---|
CSB-YP711391SWC CSB-EP711391SWC CSB-BP711391SWC CSB-MP711391SWC CSB-EP711391SWC-B |
Recombinant Saimiri boliviensis boliviensis Caspase-3(CASP3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP3 Proteins for Mesocricetus auratus (Golden hamster)
Code | Product Name | Source |
---|---|---|
CSB-YP733670MRG CSB-EP733670MRG CSB-BP733670MRG CSB-MP733670MRG CSB-EP733670MRG-B |
Recombinant Mesocricetus auratus Caspase-3(CASP3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP3 Proteins for Felis catus (Cat) (Felis silvestris catus)
Code | Product Name | Source |
---|---|---|
CSB-YP836613CA CSB-EP836613CA CSB-BP836613CA CSB-MP836613CA CSB-EP836613CA-B |
Recombinant Cat Caspase-3(CASP3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP3 Proteins for Sus scrofa (Pig)
Code | Product Name | Source |
---|---|---|
CSB-YP850191PI CSB-EP850191PI CSB-BP850191PI CSB-MP850191PI CSB-EP850191PI-B |
Recombinant Pig Caspase-3(CASP3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP3 Proteins for Canis lupus familiaris (Dog) (Canis familiaris)
Code | Product Name | Source |
---|---|---|
CSB-YP850752DO CSB-EP850752DO CSB-BP850752DO CSB-MP850752DO CSB-EP850752DO-B |
Recombinant Dog Caspase-3(CASP3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP3 Proteins for Oryctolagus cuniculus (Rabbit)
Code | Product Name | Source |
---|---|---|
CSB-YP853987RB CSB-EP853987RB CSB-BP853987RB CSB-MP853987RB CSB-EP853987RB-B |
Recombinant Rabbit Caspase-3(CASP3) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CASP3 ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E08858m | Mouse Caspase 3,Casp-3 ELISA Kit |
serum, plasma, tissue homogenates | 0.078 ng/mL |
CASP3 ELISA Kit for Rattus norvegicus (Rat)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E08857r | Rat Caspase 3,Casp-3 ELISA Kit |
serum, plasma, tissue homogenates | 0.078 ng/mL |
CASP3 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E08856h | Human Caspase 3,Casp-3 ELISA Kit |
serum, plasma, cell lysates, tissue homogenates | 0.078 ng/mL |
The CASP3 encodes the caspase-3, one of the major executioner caspases [1] in the apoptotic signaling because of its role in coordinating the demolishment of cellular structures such as DNA fragmentation or membrane blebbing. Caspase-3 is produced as a zymogen in an inactive pro-form. Cleavage and activation of pro-caspase-3 are catalyzed by initiator caspases, caspase-8, and caspase-9 through an internal cleavage to separate the large and small subunits to generate the active caspase-3 heterodimer [2]. In the APAF-1/caspase-9 apoptosome-initiated caspase activation cascade, caspase-3 has been shown to mediate feedback processing on caspase-9 [3][4]. Once activated, caspase-3 cleaves Bcl-2 and Bcl-XL, which abolishes the anti-apoptotic function of these proteins and releases C-terminal fragments that are pro-apoptotic. The cleavage of ICAD (inhibitor of caspase-activated DNase) by caspase-3 prompts the release of active CAD, which cleaves DNA and promotes chromatin condensation [5]. Caspase-3 also cleaves and activates gelsolin, a protein that regulates actin dynamics. Activated gelsolin promotes both cytoplasmic and nuclear apoptosis [6]. Katelyn G. Ponder et al. proved an initial cleavage event at D9 is required to allow cleavage at D28 that causes the complete removal of the prodomain allowing for full caspase-3 activation [7].
[1] Boatright KM, Salvesen GS Mechanisms of caspase activation [J]. Curr Opin Cell Biol. 2003 Dec; 15(6):725-31.
[2] Zou, H., Henzel, et al. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3 [J]. Cell 90, 405-413 (1997).
[3] Slee EA, et al. Ordering the cytochrome c-initiated caspase cascade: Hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner [J]. J Cell Biol, 1999, 144:281-292.
[4] Srinivasula SM, Ahmad M, et al. Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization [J]. Mol Cell, 1998, 1:949-957.
[5] Enari, M. et al. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD [J]. Nature 391, 43-50 (1998).
[6] Cohen, G. M. Caspases: the executioners of apoptosis [J]. Biochem. J. 326(Pt 1), 1-16 (1997).
[7] Katelyn G. Ponder and Lawrence H. Boise The prodomain of caspase-3 regulates its own removal and caspase activation [J]. Cell Death Discovery volume 5, Article number: 56 (2019).