Code | CSB-RA833808A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
Creating the CASP3 recombinant monoclonal antibody is a precise and multi-step process. First, the CASP3 monoclonal antibody is harvested and its gene sequence is determined. Next, a vector carrying the CASP3 monoclonal antibody gene is designed and transfected into a host cell line for culturing. To synthesize the CASP3 monoclonal antibody, a synthesized peptide from human CASP3 is used as an immunogen. The CASP3 recombinant monoclonal antibody is then purified through affinity chromatography to remove any impurities and guarantee high specificity. Finally, its specificity is confirmed through ELISA, WB, and IHC assays to ensure that it accurately recognizes its target. It only detects human CASP3 protein.
CASP3 is a critical enzyme that executes the apoptotic pathway and eliminates unwanted or damaged cells during development, tissue homeostasis, and immune defense. It is also involved in neural development by regulating axonal pruning and synapse elimination. CASP3 participates in regulating the activation and differentiation of immune cells, such as T cells and B cells. Dysregulation of CASP3 can contribute to various diseases, including cancer, neurodegeneration, and autoimmune disorders.
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