Code | CSB-RA241798A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
The production of a recombinant CASP3 monoclonal antibody involves four steps. First, the CASP3 monoclonal antibody gene is sequenced. Next, the gene is cloned into a plasmid vector. Then, the recombinant vector is introduced into a host cell line. The CASP3 recombinant monoclonal antibody is then purified from the cell culture supernatant using affinity chromatography. The final step involves testing and characterizing the purified antibody. The CASP3 monoclonal antibody is derived from the CASP3 antibody-producing hybridomas and a synthesized peptide derived from human CASP3 is used as the immunogen during its production. This CASP3 recombinant monoclonal antibody is suitable for use in ELISA, WB, and IHC applications to detect human CASP3 protein.
CASP3 is a cysteine protease that plays a critical role in the execution phase of apoptosis. During apoptosis, CASP3 is activated by proteolytic cleavage and subsequently cleaves various substrates, including structural and nuclear proteins, leading to cell disassembly and death. Once activated, CASP3 cleaves specific cellular proteins and activates other caspases, ultimately leading to the dismantling and death of the cell. CASP3 also plays a role in other cellular processes such as inflammation and cell differentiation. Dysregulation of CASP3 activity has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune diseases.
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