Human Interferon β,IFN-β/IFNB ELISA Kit

Instructions
Code CSB-E09889h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interferon, beta 1,fibroblast
Alternative Names beta-interferon ELISA Kit; Fibroblast interferon ELISA Kit; IFB ELISA Kit; IFF ELISA Kit; IFN beta ELISA Kit; IFN-beta ELISA Kit; IFNB 1 ELISA Kit; IFNB ELISA Kit; IFNB_HUMAN ELISA Kit; IFNB1 ELISA Kit; Interferon beta 1 fibroblast ELISA Kit; Interferon beta ELISA Kit; Interferon beta precursor ELISA Kit; MGC96956 ELISA Kit
Abbreviation IFNB1
Uniprot No. P01574
Species Homo sapiens (Human)
Sample Types serum, urine, cell culture supernates, tissue homogenates, cerebrospinal fluid (CSF)
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-89  
1:2 Average % 100  
Range % 91-110  
1:4 Average % 97  
Range % 92-105  
1:8 Average % 93  
Range % 89-98  
Recovery
The recovery of human IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 89-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.523 2.416 2.470 2.356  
1000 2.214 2.128 2.171 2.057  
500 1.803 1.724 1.764 1.650  
250 1.261 1.225 1.243 1.129  
125 0.765 0.716 0.741 0.627  
62.5 0.463 0.445 0.454 0.340  
31.25 0.289 0.274 0.282 0.168  
0 0.115 0.113 0.114    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function Has antiviral, antibacterial and anticancer activities.
Gene References into Functions
  1. Data indicate a mechanism used by monocyte chemotactic protein-inducing protein 1 (MCPIP1) to negatively regulated type I IFN interferon-beta antiviral defense. PMID: 29920243
  2. It has been shown that HCMV has evolved mutational robustness against IFN-beta by limiting the presence of APOBEC3G hot spots in essential open reading frames of its genome. PMID: 30045985
  3. RIG-I-like receptors have a role in induction of interferon-beta1 in antiviral gene expression PMID: 29098213
  4. Transcriptomic analysis of early untreated dermatomyositis muscles revealed that the main cluster of down-regulated genes was mitochondria-related. Histochemical, electron microscopy, and in situ oxygraphy analysis showed mitochondrial abnormalities, including increased reactive oxygen species (ROS) production and decreased respiration, which was correlated with low exercise capacities and a type I IFN signature. PMID: 28623559
  5. Gas6 bound to the fiber proteins of adenovirus and suppressed IFN beta production. PMID: 29288958
  6. The overexpression of NPIPB3 restored the interferon-beta responses in severe acute respiratory syndrome coronavirus open reading frame 6 (SARS-CoV ORF6) expressing cells, indicating that the interaction of SARS CoV ORF6 and NPIPB3 reduced Type I interferon antagonism by SARS-CoV ORF6. PMID: 26320399
  7. The results demonstrate that cystatin B interferes with the STAT-1 signaling and IFN-beta-antiviral responses perpetuating HIV in macrophage reservoirs. PMID: 27137788
  8. This review briefly discusses the dysregulation of main T cell subpopulations in CNS autoimmunity and summarized the T cell targeted effects of endogenous and exogenous IFN-beta in health and EAE/MS, with emphasis on the direct actions of IFN-beta on each T cell subset involved in the disease. PMID: 27033173
  9. c-Cbl negatively regulates IFN-beta signaling and cellular antiviral response by promoting IRF3 ubiquitination and degradation. PMID: 27503123
  10. YPEL5 silencing enhanced the induction of IFNB1 by pattern recognition receptors and phosphorylation of TBK1/IKBKE kinases, whereas co-immunoprecipitation experiments revealed that YPEL5 interacted physically with IKBKE. PMID: 27705791
  11. The effect of topical TREX1 knockdown and local interferon production on HIV transmission in human cervicovaginal explants and humanized mice, is reported. PMID: 27184854
  12. The current knowledge on IFNbeta from its structure, dynamic conformation, signaling pathway, and mechanism of action to its therapeutic effects is summarized. PMID: 27572132
  13. this study shows that the IFN-beta/STAT1 pathway is dysregulated in inflammatory bowel disease PMID: 27220814
  14. results suggest that, in addition to its well-known signaling activity, IFN-beta may be directly antimicrobial and be part of a growing family of cytokines and chemokines, called kinocidins, that also have antimicrobial properties. PMID: 28411186
  15. This study demonstrates a novel pathway for elevated IFNbeta signaling in SLE that is not dependent on stimulation by immune complexes but rather is cell intrinsic and critically mediated by IFNbeta and MAVS. PMID: 28471483
  16. G45R mutation of NS1 slightly decreased NS1 binding to dsRNA but did not interfere with its suppression of RIG-I-mediated type I IFN production. PMID: 27405392
  17. Upon influenza virus infection, DPF2 dysregulated IFN-beta induction and expression of cytokines/chemokines and antiviral proteins. This study provides evidence that influenza virus utilizes DPF2 to escape host innate immunity. PMID: 28404846
  18. Overexpression of PKV VP3 blocked IFN-beta-induced activation of the STAT1/STAT2/IRF9 promoter in a dose-dependent manner. PMID: 28441586
  19. Data suggest that EV71 infection in enterocytes does not inhibit phosphorylation of STAT1/2 induced by IFN-beta, but p-STAT1/2 transport into the nucleus is significantly blocked; EV71 infection in enterocytes down-regulates expression of KPNA1 and induces degradation of cellular KPNA1 via caspase-3. [EV17 = Enterovirus 71] PMID: 28455446
  20. This study reveals that decreased induction of both PKR and p300 proteins contribute to impaired induction of IFN-beta in Chronic obstructive pulmonary disease primary bronchial epithelial cells upon influenza infection. PMID: 26807508
  21. Together, these results suggest that RSV infection of MSCs alters their immune regulatory function by upregulating IFN-b and IDO, affecting immune cell proliferation, which may account for the lack of protective RSV immunity and for chronicity of RSV-associated lung diseases such as asthma and COPD. PMID: 27695127
  22. ArfGAP domain-containing protein 2 (ADAP2) is identified as a key novel scaffolding protein that integrates different modules of the RIG-I pathway, located at distinct subcellular locations, and mediates cellular antiviral type I interferon production. PMID: 27956705
  23. TBK1 complexes required for the phosphorylation of IRF3 and the production of interferon-beta have been identified. PMID: 28159912
  24. RNAs isolated from HCV-replicating cells triggered robust IFN-beta and IFN-lambda production through MDA5. PMID: 27655134
  25. These results suggest that inhibition of RIG-I-mediated type I interferon responses by Enterovirus 71 may contribute to the pathogenesis of viral infection. PMID: 27633794
  26. the interactions of IRF1, IFN-beta and IRF5 are involved in the M1 polarization of macrophages and have antitumor functions. PMID: 27176664
  27. The results demonstrate that PASylation has a positive impact on stability, solubility, and functional activity of IFN-beta1b and potentially might improve pharmacokinetic properties of the molecule as a therapeutic agent. PMID: 27833991
  28. Data suggest that interferon beta (IFN-beta) might be involved in modulating the expressions of interferon regulatory factor 1 (IRF1) and interferon regulatory factor-5 protein (IRF5) as well as maintaining the M1 polarization status and its function. PMID: 27363262
  29. Results suggest that serum levels of IFN-beta do not correlate well with the severity of dengue illness, but there is a clear association between changes in IFN-beta levels and the days of evolution during the acute phase of the disease. PMID: 26252251
  30. Overexpression of KLF4 inhibited virus-induced activation of ISRE and IFN-b promoter in cells, while knockdown of KLF4 potentiated viral infection-triggered induction of IFNB1 and downstream genes and attenuated viral replication. PMID: 25531393
  31. These findings suggest that RNF166 positively regulates RNA virus-triggered IFN-beta production by enhancing the ubiquitination of TRAF3 and TRAF6. PMID: 26456228
  32. the suppression of type I IFN production by HTLV-1 Tax through interaction with and inhibition of TBK1 kinase that phosphorylates IRF3 PMID: 26819312
  33. IFN-b could be an effective agent not only through its cell growth inhibitory effect on GSCs but also as a means of targeting the interconversion between GSCs and non-GSCs. PMID: 26397698
  34. These results demonstrate that poliovirus infection actively suppresses the host type I interferon response by blocking activation of IRF-3 and suggests that this is not mediated by cleavage of MDA-5 or IPS-1. PMID: 26437794
  35. The Hantaan virus A9 N protein can influence the host innate immune response by regulating the activation of IFNbeta. PMID: 26196448
  36. Together, the data demonstrate that interferon-beta inhibits foamy virus early in infection and that MxB is not a restriction factor of foamy virus. PMID: 26609934
  37. The C-terminal region of Toscana Virus NSs protein is critical for interferon-beta antagonism and protein stability. PMID: 26474372
  38. These findings support a model in which a measles virus defective interfering RNA is sensed by PACT and RIG-I to initiate an innate antiviral response via activation of interferon-beta production. PMID: 26608320
  39. Data suggest microRNA-302c regulates interferon-beta production in innate/mucosal immunity partly via down-regulation of interferon regulatory factors IRF3/7; studies were conducted in lung epithelial cells infected with influenza A virus H3N2. PMID: 26602079
  40. Data show that the MAPKKK6 ASK2, a modulator of MAPKKK5 ASK1 signaling, was essential for ASK1-dependent apoptosis, but not for inducing interferon-beta (IFNB) expression. PMID: 26243192
  41. Data suggest ADAP2 (an interferon-beta stimulated gene) disrupts pathogenic internalization of some RNA viruses/virions (dengue, vesicular stomatitis) by altering ADP ribosylation factor 6-mediated processes including up-regulation of pinocytosis. PMID: 26372645
  42. Data suggest that naturally occurring iDVGs (immunostimulatory defective viral genomes) trigger robust host antiviral/innate immunity responses including/requiring up-regulation of IFNB1 and IFNL1 (interferon lambda 1) in respiratory mucosa. PMID: 26336095
  43. Data identify an IFNbeta1-dependent, cell-autonomous mechanism that contributes to the therapeutic resistance of melanoma via the PKCepsilon-ATF2 regulatory axis. PMID: 25728676
  44. Early IFN-beta induction regulated virus replication in glioblastoma cells, whereas delayed IFN-beta induction resulted in efficient virus replication in neuroblastoma cells. PMID: 25920530
  45. Virion-associated R6 the type I IFN response by preventing efficient binding of IRF3/CBP complexes to the IFN-beta promoter in the context of infection. PMID: 25972548
  46. new IFN-beta response genes were identified in B cells, with possible implications to B cell-specific functions PMID: 25025430
  47. Reduced myelin basic protein-induced CD4+ T-cell autoreactivity in interferon-beta-treated multiple sclerosis patients may be mediated by monocyte-derived interleukin-10 PMID: 25738751
  48. Influenza virus-infected cells respond with increased induction of interferon beta upon Staphylococcus aureus super-infection, however, subsequent interferon-stimulated gene expression are rather impaired due to a block of STAT1-STAT2 dimerization. PMID: 25293394
  49. Paracrine signaling increases the number of cells that express Ifnb1 over time, calibrating the immune response to viral infection. PMID: 25670204
  50. Data suggest that BRD4 (bromodomain containing 4) is essential for Toll-like receptor-stimulated interferon-beta (IFNB) gene transcription by permitting transcription factors to interact with the IFNB promoter in plasmacytoid dendritic cells. PMID: 25891802
  51. Data indicate that interferon lambda-3, interferon beta and interleukin-10 (IL-10, IFNlambda3 and IFNbeta) are up-regulated in tick-borne encephalitis. PMID: 25461389
  52. Applied an affinity proteomic approach and identified ELAVL1/Hu antigen R (HuR) as the predominant RNA-binding protein of the IFN-beta mRNA (A/U)-rich elements (AREs). PMID: 25678110
  53. study demonstrates HSV-2 US1 inhibits production of IFN-beta by suppressing activation of IFN-beta promoter by interfering with the association of nuclear IRF-3 with IRF-3-responsive domain of the the IFN-beta promoter PMID: 25712217
  54. In an expression screen for proteins that can activate the IFNB1 promoter, authors identified the ER-associated protein ZDHHC1 as a positive regulator of virus-triggered, MITA/STING-dependent immune signaling. PMID: 25299331
  55. Binding of Yellow fever virus NS5 to the IFN-activated transcription factor STAT2 only in cells that have been stimulated with IFN-beta. PMID: 25211074
  56. IFN-beta-induced apoptosis of human SH-SY5Y neuroblastoma cells is associated with a long-lasting up-regulation of p38 MAPK activity. PMID: 25086905
  57. One of the important roles of IFN-b for malignant glioma growth inhibition was anti-angiogenesis by directly inhibiting angiogenesis through downregulation of VEGF and upregulation of IP-10 and indirectly changing the tumor microcirculation PMID: 25175315
  58. HTLV-1 Tax is recruited into the TBK1/IKKvarepsilon complexes as a scaffolding-adaptor protein that enhances IFN-beta gene expression. PMID: 25531185
  59. Hepatitis B virus Pol has a role in suppression of IFN-beta production by direct interaction with STING and subsequent disruption of its K63-linked ubiquitination, providing a new mechanism to counteract the innate DNA-sensing pathways. PMID: 25505063
  60. TRIM68 targets TFG, a novel regulator of IFN production, and in doing so turns off and limits type I IFN production in response to anti-viral detection systems PMID: 24999993
  61. A20 has a novel atheroprotective function in vascular cells through modulation of IFNgamma/STAT1 signaling in an IFNbeta-dependent, but NF-kappaB-independent, manner PMID: 25217635
  62. Human cytomegalovirus upregulates expression of the lectin galectin 9 via induction of beta interferon. PMID: 25008927
  63. Overexpression of TLR3 enhanced the activity of the IFN-beta promoter and the transcription factor NF-kappa B, thereby decreasing viral protein synthesis. PMID: 24934575
  64. This work identifies novel roles for IFN-beta and RNase L in cell barrier functions that are targeted by bacterial effectors to escape host defense mechanisms and promote virulence. PMID: 24733098
  65. Adipose tissue-derived mesenchymal stem cells cultured at high density express interferon-beta which suppresses MCF-7 tumor growth. PMID: 25016057
  66. These results indicate that vp13, encoded by open reading frame 3, enhances RIG-I signaling and interferon-beta biosynthesis, which may play a role in hepatitis E virus invasion. PMID: 24850742
  67. Data suggest the mechanism of interferon beta (IFN-beta) production induced by virus. PMID: 24909279
  68. Listeria DNA is a major trigger of interferon-beta expression in human myeloid cells during infection. PMID: 24970844
  69. Murine gammaherpesvirus 68 ORF11 targets TANK binding kinase 1 to negatively regulate the host interferon beta. PMID: 24696485
  70. N protein significantly impedes the activation of the IFN-beta promoter. PMID: 24872591
  71. The role of endogenous IFN-beta in the regulation of Th17 responses in patients with relapsing-remitting multiple sclerosis PMID: 24850724
  72. After 48 weeks, peginterferon beta-1a significantly reduces relapse rate in the placebo-controlled phase of the ADVANCE trial, a phase 3 double-blind study of patients with relapsing-remitting multiple sclerosis. PMID: 24794721
  73. Insights into the molecular mechanism of interferon-beta antagonism by Japanese encephalitis virus NS5. PMID: 24166946
  74. USP25 is a novel deubiquitinating enzyme negatively regulating virus-induced interferon-beta production. PMID: 24260525
  75. Activation of the interferon beta induction cascade by influenza a viruses requires viral RNA synthesis and nuclear export. PMID: 24478437
  76. a role for miR-449b in regulation of HDAC1 and antiviral cytokine signaling PMID: 24086750
  77. Japanese encephalitis virus non-coding RNA inhibits activation of interferon beta by blocking nuclear translocation of IRF3. PMID: 23755934
  78. The inhibin-betaC subunit is down-regulated, while inhibin-betaE is up-regulated by interferon-beta1a in Ishikawa carcinoma cell line. PMID: 23580013
  79. PLpro acts as a viral deubiquitinase and suppresses production of IFN-beta by an interfering IRF3-mediated signalling pathway. PMID: 24362959
  80. Data show that cyclosporin A (CsA) inhibited rotavirus replication and restored interferon-beta (IFN-beta) expression, and promoted Interferon Regulatory Factor-5 (IRF-5) expression in a HT-29 cell line model. PMID: 23990993
  81. These data indicate that type I IFN, via an action of IFNAR1, mediates pulmonary artery hypertension. PMID: 24334027
  82. These results show that antagonism of the interferon-beta promoter is mediated by the nucleoprotein of the Crimean-Congo hemorrhagic fever virus strain Hoti. PMID: 23990053
  83. APOBEC3 upregulated by IFN-beta induce E2 hypermutation of HPV16 in cervical keratinocytes. PMID: 24227842
  84. Dengue virus infection significantly induced the expression of miR-146a, which facilitated viral replication by targeting TRAF6 and dampening IFN-beta production. PMID: 23685241
  85. Deletion of the D domain of the PD protein results in decreased viral RNA synthesis and beta interferon expression. PMID: 23686695
  86. Herpes simplex virus 1 Us11 suppresses host interferon beta1 production through the inhibition of the PACT. PMID: 24067967
  87. Herpes simplex virus 1 US3 hyperphosphorylates IRF3 and inhibits beta interferon production. PMID: 24049179
  88. The inhibition of the expression of steroidogenic enzymes by IFN-beta is a novel mechanism that may explain its inhibitory effect on cortisol production. PMID: 23507702
  89. SHP-2 phosphatase promotes cervical cancer cell proliferation through inhibiting IFN-beta production. PMID: 22889324
  90. TRIM11 negatively regulates IFN-beta production and antiviral activity by targeting TBK1. PMID: 23675467
  91. Chlamydia induces STING-mediated IFN responses through the detection of c-di-AMP in the host cell cytosol and propose that c-di-AMP is the ligand predominantly responsible for inducing such a response in Chlamydia-infected cells. PMID: 23631912
  92. HSV-1 VP16 is able to block the production of IFN-beta by inhibiting NF-kappaB p65 activation and interfering with IRF-3 to recruit its coactivator CBP, which may be important to the early events leading to HSV-1 infection. PMID: 23824799
  93. These findings demonstrate that herpes simplex virus 1 ubiquitin-specific protease UL36 removes polyubiquitin chains on TRAF3 and counteracts the IFN-beta pathway. PMID: 23986588
  94. IFNB1 mediates its antiproliferative effects independent of autophagy, while the proapoptotic function of IFNB1 is strongly enhanced in the absence of autophagy PMID: 23221969
  95. Results show that interferon regulatory factor-5 (IRF-5) and NF-kappaB p50 are key co-regulators of IFN-beta and IL-6 expression following TLR9-mediated activation of human plasmacytoid dendritic cells PMID: 23616277
  96. Patients with multiple sclerosis who are included inthe IFNB-1b treatment program and completed the study show an improvement in quality of life PMID: 22527232
  97. the IFNB1 variant, p.Ser34Arg, could affect susceptibility to cerebral malaria in Orissa and Chhattisgarh populations of India. PMID: 23333334
  98. IFNbeta induces clearance of ataxin 7 in a preclinical model, spinocerebellar ataxia (SCA)7(knock-in mice, and improves motor function. PMID: 23518714
  99. Toscana virus nonsttructural protein (NSs) affects RIG-I mediated activation of interferon beta promoter. PMID: 23552410
  100. Induction of RIG-I and IFN-beta expression is compromised in dendritic cells isolated from chronic hepatitis B patients. PMID: 22992810

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Subcellular Location Secreted
Protein Families Alpha/beta interferon family
Database Links

HGNC: 5434

OMIM: 147640

KEGG: hsa:3456

STRING: 9606.ENSP00000369581

UniGene: Hs.93177

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