Human Tumor necrosis factor soluble receptor Ⅰ,TNFsR-ⅠELISA KIT

Instructions
Code CSB-E04736h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name tumor necrosis factor receptor superfamily, member 1A
Alternative Names CD120a, FPF, MGC19588, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60, tumor necrosis factor binding protein 1|tumor necrosis factor receptor 1|tumor necrosis factor re
Abbreviation TNFRSF1A
Uniprot No. P19438
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, urine
Detection Range 78.125 pg/mL-5000 pg/mL
Sensitivity 65 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TNFsR-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 98
Range % 92-105
1:2 Average % 91
Range % 86-98
1:4 Average % 91
Range % 88-96
1:8 Average % 101
Range % 96-106
Recovery
The recovery of human TNFsR-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 87-93
EDTA plasma (n=4) 101 103-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
5000 2.568 2.632 2.600 2.450
2500 2.245 2.121 2.183 2.033
1250 1.622 1.741 1.682 1.532
625 0.977 0.955 0.966 0.816
312.5 0.660 0.626 0.643 0.493
156.25 0.392 0.366 0.379 0.229
78.125 0.261 0.254 0.258 0.108
0 0.145 0.154 0.150  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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Target Data

Function Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
Involvement in disease Familial hibernian fever (FHF); Multiple sclerosis 5 (MS5)
Subcellular Location Cell membrane, Single-pass type I membrane protein, Golgi apparatus membrane, Single-pass type I membrane protein, Secreted, Note=A secreted form is produced through proteolytic processing, SUBCELLULAR LOCATION: Isoform 4: Secreted
Database Links

HGNC: 11916

OMIM: 142680

KEGG: hsa:7132

STRING: 9606.ENSP00000162749

UniGene: Hs.279594

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