Human vimentin,VIM ELISA Kit

Instructions
Code CSB-E08982h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name vimentin
Alternative Names CTRCT30 ELISA Kit; Epididymis luminal protein 113 ELISA Kit; FLJ36605 ELISA Kit; HEL113 ELISA Kit; VIM ELISA Kit; VIME_HUMAN ELISA Kit; Vimentin ELISA Kit
Abbreviation VIM
Uniprot No. P08670
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human VIM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 86  
Range % 80-96  
1:200 Average % 94  
Range % 91-98  
1:400 Average % 87  
Range % 80-98  
1:800 Average % 100  
Range % 92-105  
Recovery
The recovery of human VIM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 80-98  
EDTA plasma (n=4) 96 90-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.654 2.621 2.638 2.532  
1000 2.224 2.302 2.263 2.157  
500 1.536 1.487 1.512 1.406  
250 0.912 0.856 0.884 0.778  
125 0.446 0.421 0.434 0.328  
62.5 0.256 0.241 0.249 0.143  
31.25 0.137 0.145 0.141 0.035  
0 0.105 0.107 0.106    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Gene References into Functions
  1. This study measured the effects of vimentin expression on the mechano-elastic and migratory properties of the highly invasive breast carcinoma cell line MDA231. It demonstrated that vimentin stiffens cells and enhances cell migration in dense cultures, but exerts little or no effect on the migration of sparsely plated cells. PMID: 29022351
  2. Positive vimentin expression could serve as a poor prognostic marker in gastric cancer[review, meta-analysis] PMID: 30078472
  3. High vimentin expression is associated with pancreatic cancer. PMID: 29956814
  4. the HDAC inhibitors augmented both Ecadherin and vimentin expression and their effects varied in different cholangiocarcinoma cell lines. Therefore, the clinical use of HDAC inhibitors in biliary cancer should be considered cautiously PMID: 29767267
  5. miR-373 suppresses gastric cancer metastasis by downregulating vimentin. PMID: 29257346
  6. Vimentin role in the M2BP inhibition of the HIV-1 virion production.M2BP mediates the interaction between HIV-1 Gag and Vimentin. PMID: 27604950
  7. Desmin, Glial Fibrillary Acidic Protein, Vimentin, and Peripherin are type III intermediate filaments that have roles in health and disease [review] PMID: 29196434
  8. Silencing of Vimentin in CNE2 cells leads to a decrease of microvessel density and VEGF, CD31, MMP2, and MMP9 expressions in pulmonary metastatic tumors. PMID: 28744809
  9. Carcinoid-like/labyrinthine pattern of cell arrangement in vimentin/cytokeratin 20 expressing sebaceous neoplasms may represent a morphological phenotype of sebaceous mantles. PMID: 28027080
  10. Cell surface vimentin mediates DENV-2 infection of vascular endothelial cells. PMID: 27910934
  11. HIF-1alpha expression was upregulated in the vasculogenic mimicry-positive CRC cell line HCT-116 and thereby affected the expression of the EMT-related markers Claudin-4, E-cadherin (E-cd) and Vimentin(VIM). PMID: 27869227
  12. our results demonstrated that vimentin in human GC tissues and cell lines was upregulated due to its de-ubiquitination after interactions with USP14 and miR-320a, which could promote the aggressiveness of GC cells. PMID: 27448976
  13. Knocking down long pentraxin-3 (PTX3) or vimentin repressed oleate-induced head and neck squamous cell carcinomas (HNSCCs) invasion. PMID: 28489600
  14. stromal vimentin expression is a promising indicator for survival prediction and adjuvant chemotherapy response in patients with stage II colorectal cancer with high-risk factors for recurrence. PMID: 28611349
  15. Vimentin and its interaction with Shigella flexneri IpaC are dispensable for effector translocation pore formation, but are required for stable docking of Shigella flexneri to cells; moreover, stable docking triggers effector secretion. PMID: 27572444
  16. the elongation reaction of vimentin in solution and in situ by time-resolved static and dynamic light scattering, is reported. PMID: 27655889
  17. Vimentin induced by exosomes is necessary for lung cancer to induce mesenchymal transition (EMT) in recipient bronchial epithelial cells (HBECs). PMID: 27363026
  18. our results demonstrate that vimentin silencing in ovarian cancer cells upregulates proteins of the exocytotic process to decrease cellular cisplatin accumulation PMID: 27322682
  19. The combined biomarkers E-cadherin, membranous epidermal growth factor receptor (EGFR) and vimentin show a stronger prognostic value for and disease-free survival than any of the single biomarkers. PMID: 27172790
  20. AHR protein-vimentin protein complex is formed in the cytoplasm resulting in proteasome degradation of vimentin. PMID: 27752740
  21. TRIM56 is the ubiquitin ligase that is degrading vimentin in ovarian cancer cells, regulation cell migration and neoplasm invasiveness. PMID: 28771721
  22. TIS21 attenuated Doxorubicin-induced cancer cell senescence by inhibiting linear actin nucleation via Nox4-ROS-ABI2-DRF signal cascade PMID: 27932314
  23. this study indicates that OPN can induce epithelial-mesenchymal transition of hepatocellular carcinoma cells through increasing vimentin stability PMID: 26824421
  24. The findings support a mechanism in which miR-375 suppresses RUNX1 levels, resulting in reduced vimentin and L-plastin expression. Knockdown of RUNX1, L-plastin, and vimentin resulted in significant reductions in cell invasion in vitro, indicating the functional significance of miR-375 regulation of specific proteins involved in head and neck squamous cell carcinoma (HNSCC) invasion. PMID: 28499703
  25. Vimentin expression was an adverse prognostic factor for DSS in TSCC patients, even after the adjustment for cell differentiation, pathological stage, and expression levels of Snail, Twist, E-cadherin, and N-cadherin. Snail, E-cadherin, N-cadherin, and Vimentin were associated with tumorigenesis and pathological outcomes PMID: 28570699
  26. circulating anti-vimentin IgG autoantibody levels are much greater in idiopathic pulmonary fibrosis subjects than in normal controls PMID: 28754682
  27. RhoA/ROCK and Raf-1/CK2 pathway are responsible for TNF-alpha-mediated endothelial cytotoxicity via regulation of the vimentin cytoskeleton. PMID: 28743511
  28. It was concluded that islet cell expression of vimentin indicates a degree of plasticity and dedifferentiation with potential loss of cellular identity in diabetes. PMID: 28348116
  29. findings have identified a role for members of these signaling pathways in the regulation of EGF-induced vimentin expression in the MDA-MB-468 breast cancer cell line PMID: 27163529
  30. changes in methylation levels in cfDNA associated with hepatocellular carcinoma and could represent useful plasma-based biomarker PMID: 28333958
  31. vimentin regulates the differentiation switch via modulation of K5/K14 expression. Moreover, because there was a significant correlation between high vimentin-K14 expression and recurrence/poor survival in oral cancer patients, vimentin-K14 together may prove to be the novel markers for the prognostication of human oral cancer. PMID: 28225793
  32. Data indicate that ellagic aicd (EA) down-regulates the expression of COX-2, NF-kappa B, vimentin and up-regulates the expression of E-cadherin in in pancreatic carcinom PANC-1 cells. PMID: 28135203
  33. Bevacizumab treatment was also associated with structural protein abnormalities, with decreased GFAP and vimentin content and upregulated GFAP and vimentin mRNA expression. PMID: 28419863
  34. Decreasing cell surface vimentin by small interfering RNA (siRNA) knockdown in HeLa and NIKS cells significantly increased human papillomavirus 16 infectious internalization, while overexpression of vimentin had the opposite effect identifying vimentin as a viral restriction factor. PMID: 28566373
  35. From these data, we suggest that filamentous vimentin underneath the plasma membrane is involved in increasing integrin adhesiveness, and thus regulation of the vimentin-integrin interaction might control cell adhesion PMID: 27044755
  36. depletion induces phosphorylation of the microtubule-associated GEF-H1 on Ser886, and thereby promotes RhoA activity and actin stress fiber assembly. PMID: 28096473
  37. Data show that the filament elongation of both desmin and keratin K8/K18 proceeds very similar to that of vimentin. PMID: 27304995
  38. Coexistence of vimentin-positive and Axl-high expression is a poor prognostic factor for primary breast cancer. Vimentin and Axl expression might contribute to the aggressive phenotype in breast cancer. PMID: 27506606
  39. our findings suggest that the HIF-1alpha-HDAC1 complex transcriptionally inhibits miR-548an expression during hypoxia, resulting in the upregulation of vimentin that facilitates the pancreatic tumorigenesis. PMID: 27353169
  40. Rab7a depletion decreases the amount of active Rac1 but not its abundance and reduces the number of cells with vimentin filaments facing the wound, indicating that Rab7a has a role in the orientation of vimentin filaments during migration PMID: 27888097
  41. Over-expression of TNC, SMA, and vimentin were significantly correlated with the lower overall survival in prostate cancer patients. PMID: 28341124
  42. Results show that VIM mRNA is regulated in melanoma by UNR protein. PMID: 27908735
  43. These findings suggest that Plk1 regulates smooth muscle contraction by modulating vimentin phosphorylation at Ser-56. PMID: 27662907
  44. Structural Dynamics of the Vimentin Coiled-coil Contact Regions Involved in Filament Assembly as Revealed by Hydrogen-Deuterium Exchange.( PMID: 27694444
  45. These findings are the first to demonstrate that vimentin is critical for influenza viral infection as it facilitates endosomal trafficking and acidification, and mediates viral genome penetration into the cytoplasm to propagate the infection. PMID: 27423069
  46. RAP1 promotes colorectal cell migration through the regulation of Vimentin and RAP1 may act as a potential target for the diagnosis and therapy of CRC. PMID: 28381157
  47. High vimentin expression is associated with Non Small-cell Lung Cancer. PMID: 28373440
  48. data demonstrates that keratinocyte migration requires the interaction between vimentin and keratins at the -YRKLLEGEE- sequence at the helical 2B domain of vimentin PMID: 27072292
  49. High vimentin expression is associated with malignant pleural mesothelioma. PMID: 27646775
  50. Increased TET1 induced re-expression of vimentin through active DNA demethylation, and cause partial epithelial-to-mesenchymal (EMT) in A2780 cells. PMID: 28150354

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Involvement in disease Cataract 30, multiple types (CTRCT30)
Subcellular Location Cytoplasm
Protein Families Intermediate filament family
Tissue Specificity Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Database Links

HGNC: 12692

OMIM: 116300

KEGG: hsa:7431

STRING: 9606.ENSP00000224237

UniGene: Hs.455493

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