Human vimentin,VIM ELISA Kit

Code CSB-E08982h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P08670
Lead Time 3-5 working days
Abbreviation VIM
Protein Biological Process 1 Immunity
Target Name vimentin
Alias RP11-124N14.1, FLJ36605,
Species Homo sapiens (Human)
Protein Biological Process 3 Host-virus interaction
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 12692
RGD 621646
MGI 98932
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human VIM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 86  
Range % 80-96  
1:200 Average % 94  
Range % 91-98  
1:400 Average % 87  
Range % 80-98  
1:800 Average % 100  
Range % 92-105  
Recovery
The recovery of human VIM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 80-98  
EDTA plasma (n=4) 96 90-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.654 2.621 2.638 2.532  
1000 2.224 2.302 2.263 2.157  
500 1.536 1.487 1.512 1.406  
250 0.912 0.856 0.884 0.778  
125 0.446 0.421 0.434 0.328  
62.5 0.256 0.241 0.249 0.143  
31.25 0.137 0.145 0.141 0.035  
0 0.105 0.107 0.106    
References
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Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involvement in disease Cataract 30, multiple types (CTRCT30)
Subcellular Location Cytoplasm
Protein Families Intermediate filament family
Tissue Specificity Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Database Links

HGNC: 12692

OMIM: 116300

KEGG: hsa:7431

STRING: 9606.ENSP00000224237

UniGene: Hs.455493

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