Rat Angiotensin converting enzyme 2, ACE2 ELISA Kit

Instructions
Code CSB-E14308r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Alternative Names ACEH, DKFZp434A014, ACE-related carboxypeptidase|angiotensin I converting enzyme 2|angiotensin converting enzyme-like protein
Abbreviation ACE2
Uniprot No. Q5EGZ1
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 IU/mL-100 IU/mL
Sensitivity 0.39 IU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat ACE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %87-95
1:2Average %89
Range %85-94
1:4Average %93
Range %89-97
1:8Average %96
Range %92-100
Recovery
The recovery of rat ACE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8480-88
EDTA plasma (n=4)9284-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
IU/mlOD1OD2AverageCorrected
1001.997 1.950 1.974 1.810
501.312 1.373 1.343 1.179
250.954 0.897 0.926 0.762
12.50.589 0.603 0.596 0.432
6.250.411 0.409 0.410 0.246
3.130.297 0.289 0.293 0.129
1.560.204 0.197 0.201 0.037
00.168 0.159 0.164  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Target Data

Function Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function.
Subcellular Location Processed angiotensin-converting enzyme 2: Secreted, SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein, Cytoplasm
Protein Families Peptidase M2 family
Tissue Specificity Expressed in heart, kidney and forebrain. In testis, expression is restricted to Leydig cells. In heart, expressed in endothelial cells from small and large arteries, arterial smooth muscle cells, and myocytes (at protein level). Ubiquitously expressed, w
Database Links

KEGG: rno:302668

STRING: 10116.ENSRNOP00000047913

UniGene: Rn.230337

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