Rat Angiotensin converting enzyme 2, ACE2 ELISA Kit

Code CSB-E14308r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q5EGZ1
Lead Time 3-5 working days
Abbreviation ACE2
Target Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Alias ACEH, DKFZp434A014, ACE-related carboxypeptidase|angiotensin I converting enzyme 2|angiotensin converting enzyme-like protein
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 IU/mL-100 IU/mL
Sensitivity 0.39 IU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
HGNC 13557
RGD 728890
MGI 1917258
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat ACE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %87-95
1:2Average %89
Range %85-94
1:4Average %93
Range %89-97
1:8Average %96
Range %92-100
Recovery
The recovery of rat ACE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8480-88
EDTA plasma (n=4)9284-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
IU/mlOD1OD2AverageCorrected
1001.997 1.950 1.974 1.810
501.312 1.373 1.343 1.179
250.954 0.897 0.926 0.762
12.50.589 0.603 0.596 0.432
6.250.411 0.409 0.410 0.246
3.130.297 0.289 0.293 0.129
1.560.204 0.197 0.201 0.037
00.168 0.159 0.164  
References
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Function Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function.
Subcellular Location Processed angiotensin-converting enzyme 2: Secreted, SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein, Cytoplasm
Protein Families Peptidase M2 family
Tissue Specificity Expressed in heart, kidney and forebrain. In testis, expression is restricted to Leydig cells. In heart, expressed in endothelial cells from small and large arteries, arterial smooth muscle cells, and myocytes (at protein level). Ubiquitously expressed, w
Database Links

KEGG: rno:302668

STRING: 10116.ENSRNOP00000047913

UniGene: Rn.230337

Pathway Renin-angiotensin system

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