Rat Angiotensin converting enzyme 2, ACE2 ELISA Kit

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Target Background

Name: Rat Angiotensin converting enzyme 2, ACE2 ELISA Kit
Brand: CUSABIO
SKU: CSB-E14308r

Product Details

Alternative Names

Ace2Angiotensin-converting enzyme 2 ELISA kit; EC 3.4.17.23 ELISA kit; ACE-related carboxypeptidase) [Cleaved into: Processed angiotensin-converting enzyme 2] ELISA kit

Abbreviation

ACE2

Uniprot No.

Q5EGZ1

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.027 ng/ml-20 ng/ml

Sensitivity

0.027 ng/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ACE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	99
	Range %	94-103
1:2	Average %	105
	Range %	98-112
1:4	Average %	93
	Range %	89-98
1:8	Average %	96
	Range %	91-101

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

1.896

1.934

1.915

1.824

6.66

1.134

1.167

1.151

1.060

2.22

0.678

0.708

0.693

0.602

0.74

0.483

0.509

0.496

0.405

0.24

0.292

0.301

0.297

0.206

0.082

0.171

0.173

0.172

0.081

0.027

0.107

0.112

0.109

0.018

0.089

0.093

0.091

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The product CSB-E14308r is a sandwich ELISA kit developed to measure levels of rat angiotensin-converting enzyme 2 (ACE2) in serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of ACE2 bound in the initial step.

ACE2 is a negative regulator of the renin-angiotensin system (RAS) and contributes to catalyzing the conversion of angiotensin II to angiotensin 1-7, thereby counterbalancing ACE activity. Increasing evidence demonstrates that the enzymatic activity of ACE2 plays a protective role in cardiovascular diseases. Loss of ACE2 can be detrimental, as it results in functional deterioration of the heart and progression of cardiac, renal, and vascular pathologies. ACE2 also acts as the key SARS coronavirus receptor and stabilizer of neutral amino acid transporters. ACE2 has recently been shown to act as a major receptor for the SARS-CoV-2 S protein enabling viral fusion and entry into the host cells.

Target Background

Function
(From Uniprot)

Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins, casomorphins and dynorphin A. Plays an important role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 in intestine, regulating trafficking, expression on the cell surface, and its catalytic activity.

Gene References into Functions

ACE2 significantly attenuated lipopolysaccharide- induced lung injury and suppressed inflammatory response.AEC2 attenuates acute respiratory distress syndrome by inhibiting ERK/NF-kappaB activation in the lung. PMID: 27302421
Activation of ACE2 had a protective role in the development of pulmonary arterial hypertension in rat model via improving the function of pulmonary arterial endothelium. This effect was potentially mediated by promoted NO release as a consequence of increased phosphorylation of eNOS at Ser1177 and dephosphorylation of eNOS at Thr495. PMID: 29146157
These results suggest that insufficiency of hypothalamic ACE2 is associated with hypertension in SHR rats. PMID: 28423630
ACE inhibitory peptide IRW could contribute to its antihypertensive activity through increased ACE2 expression and decreased proinflammatory genes expression in mesenteric arteries. PMID: 26016560
the relationship between ACE and ACE2 activity, left ventricular function and gender in spontaneously hypertensive rats, was determined. PMID: 26010093
ACE and ACE 2 are expressed in lipopolysaccharide-induced lung injury and rebalanced by ACE inhibitor captopril PMID: 25768373
In experimental ischemic stroke, cerebral cortex and striatum ACE2 increased in the 1st 24 hr. Serum ACE2 activity decreased within 4 hr post-stroke, but reached higher than baseline levels after 3 days. PMID: 25941346
The study showed that both ACE and ACE-2 are overexpressed in the cardiac and renal tissues of animals with heart failure as compared with their sham controls. PMID: 25217176
aortic constriction results in cardiac hypertrophy, fibrosis and a rise of cardiac ACE expression. PMID: 25575522
Activation of ACE2 has a beneficial effect in hyperglycaemic rats, improving the cardiac electrical function. PMID: 24741027
The increase in Ace mRNA levels was higher than that of Ace2 mRNA levels.Together, these data suggest that the ACE-Ang II-AT1 axis is the dominant axis in severe kidney injury PMID: 24781988
Thus we may draw the conclusion that TIIA may exert protective effects on BLM induced PF in rats, and the ACE-2/ANG-(1-7) axis may ascribe to those protective effects. PMID: 24782646
Ace2 expression is upregulated in insulin-sensitive tissues following high-fat diet-induced nonalcoholic steatohepatitis. PMID: 24558317
Angiotensin-converting enzyme-2 overexpression attenuates inflammation in rat model of chronic obstructive pulmonary disease. PMID: 24417403
Hepatic ACE2 expression markedly increased in rats with high fat diet-induced NASH and was further upregulated by pioglitazone. PMID: 24114819
Long term AT2 receptor activation increases renal ACE2 activity, attenuates AT1 receptor function and decreases blood pressure in obese Zucker rats. PMID: 23823602
ACE2 activation by resorcinolnaphthalein improved endothelial function and suppressed neointimal formation in the prevention of severe PAH by the mechanism of mediating the levels of the components of the renin-angiotensin system. PMID: 23652350
Enalapril decreases cardiac ACE levels in spontaneously hypertensive rats. PMID: 23706365
Intimal thickening after balloon injury is linked with reduced expression of ACE2.Valsartan can inhibit the intimal thickening possibly by upregulating ACE2 and Ang(1-7) and downregulating AT(1). PMID: 23141017
In rats with chronic kidney disease, renal ACE2 deficiency and lack of activation of cardiac ACE2 may contribute to progression of cardiac and renal tissue injury. PMID: 22198016
Apigenin reduces blood pressure in SHR rats by upregulating ACE2 in the kidney. PMID: 21351633
The coronary vasoconstriction observed in diabetic rats was unchanged by angiotensin-converting enzyme 2 activation. PMID: 22286369
Data suggest that strategies that do augment angiotensin-converting enzyme 2 (ACE2) after myocardial infarction (MI) may be a useful addition to standard renin-angiotensin system blockade after MI. PMID: 22715807
Data suggest that jejunal enterocyte protein and mRNA expression of ACE2 and Mas receptor are up-regulated in type 1 diabetes. PMID: 22811473
The expressions of ACE and ACE2 mRNA in lung tissue of the rats with paraquat poisoning are decreased. PMID: 20465954
Activation of intrinsic cardiac ACE2 by oral XNT (1-[[2-(dimethylamino)ethyl]amino]-4-(hydroxymethyl)-7-[[(4-methylphenyl)sulfonyl]oxy]-9H-xanthen-9-one) treatment protects the heart against diabetes-induced dysfunction. PMID: 22595130
results demonstrated that activation of ACE2 by continuous injection of resorcinolnaphthalein prevented the development of pulmonary arterial hypertension through improving early endothelial dysfunction PMID: 22613639
culating angiotensin-converting enzyme 2 increased ninefold in diabetic females and threefold in diabetic males PMID: 22378820
ACE-2/ACE protein expression ratio was also disrupted with a significant reduction in adjuvant arthritis animals. ACE-2 has been found to provide negative feedback of renin-angiotensin system and protection of the heart and kidneys. PMID: 21053061
ACE2 inhibits myocardial collagen accumulation and improves LV remodeling and function in a rat model of diabetic cardiomyopathy. PMID: 22340266
ACE2 overexpression in the paraventricular nucleus has beneficial effects in counteracting Ang II-induced hypertension. PMID: 21952934
Data highlight the importance of increased expression and subsequent interaction of ACE2 and nNOS within the paraventricular nucleus, leading to a reduction in sympathetic outflow in congestive heart failure. PMID: 21963832
Results demonstrate that mouse and rat ACE2 are less sensitive to inhibition by DX600 than human ACE2. PMID: 21880865
Feeding flavonoid-rich blueberries to spontaneously hypertensive stroke-prone rats lowers blood pressure via inhibition of ACE2 activity. PMID: 21186379
Irbesartan can increase ACE2 mRNA expression in diabetic rat myocardium. PMID: 20584672
Ang-(1-7) Mas receptors and AT2 receptors in hypothalamic paraventricular nucleus neurons play role in mediating tonic maintenance of renal sympathetic nerve activity. (Angiotensin 1-7) PMID: 16027241
The attenuated counterbalancing effect of Ang-(1-7) on baroreflex function is lost in older rats, which may be attributable to diminished production of the peptide from neprilysin. (Angiotensin 1-7) PMID: 16009784
Findings suggest that the transactivation of -converting enzyme 2 and the reduction in brain angiotensin II are ERalpha dependent. PMID: 21536991
Panax notoginseng saponins can stimulate ACE2 to inhibit the expression of TNF-alpha and enhance antioxidation in rats with post-myocardial infarction-ventricular remodeling. PMID: 20518313
ACE2 inhibition increased thrombus weight by 30% and XNT treatment resulted in a 30% attenuation of thrombus formation in the SHR. ACE2 activation attenuates thrombus formation and reduces platelet attachment to vessels. PMID: 20111697
renal ACE2 is not altered in the SHRSP and TGR(mREN2)27 genetic rat models with established hypertension. PMID: 19927150
The downregulation of ACE2 mRNA and protein expression in rat pulmonary microvascular endothelial cells by endotoxin may play an important role in pathogenesis of acute lung injury. PMID: 18926065
Angiotensin-converting enzyme 2 (Ace2)is an essential regulator of heart function. PMID: 12075344
Enhanced renal expression of ACE2 during pregnancy. PMID: 12874086
Upregulation of ACE 2, through increased conversion of Ang II into Ang-(1-7) may counterbalance the vasopressor effects of ACE that are mediated by Ang II formation. PMID: 15007027
ACE2 is a constitutive product of adult-type Leydig cells and may participate in the control of testicular function by as yet unknown mechanisms. PMID: 15231706
These data represent the first report of ACE2 and angiotensin-(1-7) expression in the aorta and carotid arteries of SHR rats PMID: 15833808
ACE2 activity and mRNA level are highest in the ileum and kidney compared to other organs. PMID: 15949646
The modulatory role of angiotensin-(1-7)in the transcriptional regulation of angiotensin-converting enzyme 2 by angiotensin II suggests a complex interplay between these peptides that is mediated by distinct receptor systems. PMID: 16176966
ACE2 and ACE are coexpressed in numerous rat tissues, and their increased activity in the lung of FR30 rats may participate in hypertension programming. PMID: 16203874

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Subcellular Location

[Processed angiotensin-converting enzyme 2]: Secreted.; Cell membrane; Single-pass type I membrane protein. Cytoplasm. Cell projection, cilium. Apical cell membrane.

Protein Families

Peptidase M2 family

Tissue Specificity

Expressed in heart, kidney and forebrain. In testis, expression is restricted to Leydig cells. In heart, expressed in endothelial cells from small and large arteries, arterial smooth muscle cells, and myocytes (at protein level). Ubiquitously expressed, w

Database Links

KEGG: rno:302668

STRING: 10116.ENSRNOP00000047913

UniGene: Rn.230337

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Code	CSB-E14308r
Size	96T,5×96T,10×96T
Price	Request a Quote
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.