Recombinant Human Beta-nerve growth factor(NGF) (Active)

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Code CSB-AP003771HU
Size US$2376Purchase it in Cusabio online store
(only available for customers from the US)
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity The ED50 as determined in a cell proliferation assay using TF?1 human erythroleukemic cells is less than 2 ng/ml.
Target Names NGF
Uniprot No. P01138
Research Area Neuroscience
Alternative Names Beta nerve growth factor; Beta NGF; Beta-nerve growth factor; Beta-NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor (beta polypeptide); Nerve growth factor; Nerve growth factor beta; Nerve growth factor beta polypeptide; Nerve growth factor beta subunit; NGF; NGF_HUMAN; NGFB; NID67
Species Homo sapiens (Human)
Source E.coli
Expression Region 122-241aa
Mol. Weight 13.4 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 μm filtered 20 mM PB, 250 mM NaCl, pH 7.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI
Gene References into Functions
  1. the effects of secretome-enriched conditioned medium (CM) of neuronally differentiated PC12 cells on the neuronal differentiation of human mesenchymal stem cells via NGF like mechanism, is reported. PMID: 29527653
  2. Single nucleotide polymorphisms in NGF gene (rs6330) and NGFR gene (rs2072446 and rs734194) are associated with ischemic stroke. The NGF rs6330*T and NGFR rs2072446*T minor alleles might be nominated as a risk factor for developing ischemic stroke and NGFR rs734194*G minor allele as a protective against this disease at least in Armenian population PMID: 29499660
  3. Neurotrophic factors and hippocampal activity in PTSD PMID: 29799860
  4. SNRPA may contribute to GC progression via NGF and could be a prognostic biomarker for GC. PMID: 30039889
  5. Results show that NGF signalling is strongly linked to pathological and regenerative processes in human teeth. PMID: 28465581
  6. The results suggest an interaction between NGF, GDNF and MMP-9 during the transition to malignancy in prostate cancer (PC). Also this interaction may involve in regulating PC cell differentiation, tumor invasion, progression, and the agressiveness of PC. PMID: 28237042
  7. the anti-tumor activity of oleuropein against hepatocellular carcinoma could be attributed to influencing the pro-NGF/NGF balance via affecting MMP-7 activity without affecting the gene expression of NGF PMID: 29476769
  8. co-expression patterns of NGF and heme oxygenase-1 might be used as prognostic indicators for gastric carcinoma patients PMID: 28679437
  9. This review will briefly address the peripheral and central sensitization mechanisms of airway neurons and will then focus on NGF signaling and its role in cough hypersensitivity.[review] PMID: 28494216
  10. First-trimester plasma nerve growth factor is lower in patients who subsequently develop preeclampsia. PMID: 27513943
  11. This study show evidence of variation in plasmatic beta-NGF expression during the progression of dementia. PMID: 27802234
  12. NGF is functionally linked to beta-catenin, promoting the migration of human ovarian cancer cells via the WNT/beta-catenin pathway. PMID: 27835587
  13. Varicella zoster virus DNA replication is regulated in part by an NGF pathway that is PI3-kinase-independent. PMID: 27683235
  14. Many studies indicate that the only presence of NGF is unable to generate cell carcinogenesis, both in normal neuronal and non-neuronal cells/tissues. However, it cannot be excluded the possibility that the co-expression of NGF and pro-carcinogenic molecules might open to different consequence. [review] PMID: 27439311
  15. the effect and underlying mechanisms of NGF/BDNF on the production of NPW in PC12 cells and hypothalamus, is reported. PMID: 28249734
  16. These data support a role for islet NGF in fine-tuning insulin secretion. PMID: 27424144
  17. The results of this study indicated that dysmenorrhea pain severity is partly genetically determined by the chromosome 1p13.2, near the nerve growth factor locu. PMID: 27454463
  18. Findings suggest that IL-1beta and TNF-alpha regulate Nerve Growth Factor expression and production in synovial macrophages and fibroblasts in osteoarthritic joints. PMID: 28677145
  19. These results advance our knowledge of the conformational properties of proNGF and NGF and help provide a rationale for the diverse biological effects of NGF and proNGF at the molecular level. PMID: 28798232
  20. The results of this study suggest that proNGF protein levels may augment the diagnostic accuracy of currently used CSF biomarker panels. PMID: 26825093
  21. Neuroimmune-endocrine events may lead to overactivity of sympathetic nervous system that triggers cascade of pathologic conditions in ovary in polycystic ovary syndrome (PCOS). Data suggest women with PCOS exhibit reduction of CRH and NGF; reduction of CRH/NGF may be under influence of sympathetic nervous system and may reflect deficit of neuronal stress-adaptation in PCOS patients. (CRH = corticotropin releasing hormone) PMID: 27908212
  22. The rare nerve growth factor-beta (NGFB) mutation R221W causes a selective loss of thinly myelinated fibers and especially unmyelinated C-fibers. Carriers of this mutation show altered pain sensation. PMID: 27146986
  23. NGF expression was positively correlated with disease severity and visceral hypersensitivity in irritable bowel syndrome patients. PMID: 27862119
  24. a stage-related modulation of beta-NGF and its receptors in the inflammatory process of OA PMID: 28253191
  25. BDNF and NGF serum levels are reduced in the early and moderate glaucoma stages, suggesting the possibility that both factors could be further investigated as potential circulating biomarkers for the early detection of glaucoma. PMID: 28068360
  26. overexpression of ARMS blocked NGF-mediated secretion, without affecting basal secretion, a decrease in ARMS resulted in potentiation. Similar effects were observed with synembryn-B, a protein that interacts directly with ARMS. Downstream of ARMS and synembryn-B are Galphaq and Trio proteins, which modulate the activity of Rac1 in response to NGF PMID: 26966186
  27. Data show that the standardized nerve growth factor (NGF) concentration was negatively correlated with continuous pain, neuropathic pain and total score, and the standardized S100 proteins S100A8/A9 concentration was positively correlated with present pain intensity and continuous pain. PMID: 27936243
  28. Results indicate that NGF inhibited CRT translocation induced by mitoxantrone. NGF effect on CRT translocation could have consequences in immunotherapy, potentially lessening the effectiveness of this type of treatment. PMID: 28260038
  29. NGF stimulates generation of neurons, but not neuronal progenitors from embryonic stem cells and affects the proportion of specific types of neurons in cultures of differentiating embryonic stem cells. PMID: 28364186
  30. NGF attenuates these responses-both in vivo and in vitro. Therefore, NGF therapy may represent a novel approach for the management of diabetic keratopathy. PMID: 27978558
  31. NGF signaling pathway provides a potential target for developing molecularly targeted therapies PMID: 27792755
  32. these data suggest a positive feedback loop through which NGF-mediated upregulation of p75(NTR) can contribute to the chemo-resistance of Triple negative breast cancer cells. PMID: 27577679
  33. Based on these current developments, the present review provides not only a broad overview of the biological effects of NGF-TrkA-p75NTR on cancer cells and their microenvironment, but also explains why NGF and its receptors are going to evoke major interest as promising therapeutic anti-cancer targets in the coming decade. PMID: 27264679
  34. NGF/CD133 might be an effective and potent therapeutic target for pancreatic cancer metastasis, particularly in perineural invasion PMID: 27654574
  35. The analysis of covariance (ANCOVA) indicated that the mean serum GDNF and NTF3 levels of ADHD patients were significantly higher than that of controls. However, serum BDNF and NGF levels did not show any significant differences between groups. PMID: 27561780
  36. All patients had serum neurotrophin (NT-3, BDNF, NGF) concentrations determined. PMID: 27367919
  37. Serum NGF does not differentiate between recurrent acute pancreatitis and chronic pancreatitis. PMID: 27020638
  38. NGF FLIPs TrkA onto the death TRAIL in neuroblastoma cells PMID: 26962689
  39. Major depression patients had similar serum NGF to controls. PMID: 27008247
  40. data demonstrate the involvement and modulation of nerve growth factor and its receptors in chronic obstructive pulmonary disease and in its staging PMID: 26408608
  41. The present study showed that IL-17, in addition to stimulating an inflammatory response, negatively regulates the action of NGF and NGF R in the polar forms of the leprosy. PMID: 26616164
  42. Locally increased estrogen levels and inflammation may cause increased NGF production in the uterus of patients with adenomyosis. PMID: 25519715
  43. The present study is the first to thoroughly assess the enhancement of neural differentiation of bone marrow mesenchymal stem cells following transfection with bFGF and NGF. PMID: 26572749
  44. Data suggest MMP7 (matrix metalloproteinase 7) in follicular fluid cleaves proNGF (pro-nerve growth factor) in ovarian follicle; both MMP7 and proNGF appear to be products of granulosa cells; processing of proNGF to NGF appears to regulate apoptosis. PMID: 26457789
  45. NGF promotes renal fibrosis via TGF-beta1-signaling activation, suggesting that in kidney diseases high NGF serum levels could contribute to worsen renal fibrosis. PMID: 26066770
  46. This study suggests that the circadian rhythm in the esophagus may be important for the mediation of and/or the response to erosive damage in GERD patients. PMID: 26337663
  47. Intratumoral nerve growth factor expression is not associated with perineural invasion in patients with resected extrahepatic cholangiocarcinoma. PMID: 26547754
  48. NGF has a role in modulating trkANGFR/p75NTR in alphaSMA-expressing conjunctival fibroblasts from human ocular cicatricial pemphigoid PMID: 26569118
  49. NGF-induced tyrosine kinase independent TrkA signaling through CD44 is sufficient to maintain tumor aggressiveness in breast cancer PMID: 25840418
  50. Urinary NGF, but not BDNF, levels decreased significantly after hyaluronic acid therapy. PMID: 24614892
  51. These results suggest that reduced NGF serum levels can be a possible biomarker of MDD. PMID: 26118751
  52. Study found that NGF and p75NTR mRNA expression levels in liver tissues with hepatic fibrosis (viral or not) were up-regulated and they can regulate the course of the disease by decreasing or inhibiting hepatocyte regeneration and proliferation. PMID: 25816145
  53. NGF and its precursor proNGF are primarily considered as regulators of neuronal function that induce their responses via the tyrosine kinase receptor TrkA and the panneurotrophin receptor p75NTR. (Review) PMID: 25491371
  54. These observations add further evidence in favor of anti-NGF therapy in autoimmune diseases and also unlock a new area of research about the role of NGF in IL-1beta mediated diseases. PMID: 25876154
  55. NGF variants are associated with remission rates after 8-week antidepressant treatment, and harm avoidance partially mediated the effect of NGF variants on treatment outcomes. PMID: 26021968
  56. Vitreous and sera from diabetic patients showed significant 44% and 64% reductions in NGF levels. PMID: 25853140
  57. Studies show that NGF stimulates lymphangiogenesis and may act as a pathogenic factor that negatively regulates the normal neural and lymphatic vascular remodeling events that accompany wound recovery. PMID: 25383879
  58. Results suggest that chronic elevation of NGF but not BDNF concentration protects long-term potentiation induction from beta-amyloid action through the PI3K-signaling cascade PMID: 25595986
  59. Significant chemical differences in NGF were associated with the clinical features of major depressive disorder. PMID: 24899094
  60. it is the oligomerization state of the secreted NGF that may drive the formation of the ternary heterocomplex. PMID: 25650935
  61. these results show that the NGF signaling and downstream pathways in the placenta are affected by SSRI treatment and/or antenatal depression. PMID: 25611484
  62. the signaling endosome model has been used to describe the sustained NGF/TrkA retrograde signaling as a process of endocytosis and retrograde transport of NGF/TrkA-containing endosomes from the axon terminal PMID: 25619719
  63. data indicate, for the first time, that the NGF/proNGF/p75(NTR) axis plays a critical role in regulating breast CSC self-renewal and plasticity. PMID: 25286822
  64. NRH2 enhanced the ratio of Bax/Bcl-2 by promoting the expressions of proNGF, sortilin and p75NTR, thereby inducing brain cell apoptosis. PMID: 25854576
  65. Report decreased urinary NGF levels after acupuncture or solifenacin treatment for overactive bladder. PMID: 25033919
  66. Despite being highly conserved, NGF and proNGF of mouse and human origins show distinct properties.Special care must be taken in performing experiments with cross-species systems. PMID: 25496838
  67. ProNGF is a biomarker associated with high-risk prostate cancers and a potential driver of infiltration by nerves PMID: 25285721
  68. polymorphism (rs6330, c.104C > T, p.Ala35Val) modulates cardiac autonomic nervous activity in a sex-specific manner PMID: 25162994
  69. Urinary levels of NGF and HB-EGF may be potential biomarkers for evaluating outcome of overactive bladder syndrome treatment. PMID: 25510766
  70. urinary NGF could be a useful biomarker for the diagnosis of overactive bladder. PMID: 25181532
  71. This study demonstrated a complex of immunochemical and immunohistochemical expression of the key adipokines including leptin, NGF and adiponectin in women with metabolic syndrome. PMID: 25916157
  72. results suggest that NGF in the intervertebral disc is related to pain generation PMID: 25069717
  73. Both BDNF and NGF serum concentrations were significantly lower among chronic ketamine users than among health controls. PMID: 25064020
  74. NGF levels showed a significant increase compared to the mean levels of the control group. PMID: 24706316
  75. No differences in plasma BDNF, NGF, NT3, NT4 and GDNF were found between autism spectrum disorder and control. PMID: 25275256
  76. Women with detrusor overactivity had significantly higher baseline urinary NGF levels compared to controls. NGF levels dropped after sacral nerve stimulation. PMID: 25019486
  77. Urinary NGF may be a biomarker for higher-grade prostate cancer aggressiveness PMID: 24794250
  78. Increased NGF expression is associated with spontaneous abortions. PMID: 24825909
  79. NGF, but not BDNF or IL6, has a role in infant motor development in children of mothers with postpartum affective disorders PMID: 24733087
  80. TRPV1-immunoreactive nerve fibers and NGF have a role in inflammation in interstitial cystitis/bladder pain syndrome PMID: 24458144
  81. Serum levels of FGF-2, NGF and IGF-1 in patients with manic episode of bipolar disorder were significantly higher than those in healthy controls PMID: 24793757
  82. NGFB and TrkA expression is elevated in salivary gland epithelial cells of primary Sjogren's syndrome. PMID: 24557415
  83. reduced serum NGF level may be a state marker of depression in elderly subjects PMID: 23570892
  84. It plays a role in the neuronal and cognitive dysfunction of Alzheimer's disease. PMID: 24112788
  85. Results suggest that nerve growth factor (NGF) and hypoxia inducible factor-1alpha (HIF-1alpha) are synergically involved in the angiogenesis of non-small cell lung cancer (NSCLC). PMID: 24939299
  86. Upregulation and maintenance of HIF-1alpha and NGF expression may attenuate VCI. PMID: 23771673
  87. Silencing beta-NGF gene expression by siRNA suppresses the growth of endometriotic implants. PMID: 23885099
  88. Neuropeptide NGF mediates neuro-immune response and inflammation through mast cell activation. PMID: 25001650
  89. the serum c-reactive protein (CRP), nerve growth factor (NGF) and pro-inflammatory cytokine/chemokine interleukin (IL)-1beta, IL-6, tumor necrosis factor (TNF)-alpha, and IL-8 expression in patients with interstitial cystitis/bladder pain syndrome, was measured. PMID: 24146927
  90. Expression of nerve growth factor and heme oxygenase-1 predict poor survival of breast carcinoma patients. PMID: 24180625
  91. Data indicate that nerve growth factor (NGF) binding to TrkA affects toll-like receptor (TLR) signaling, favoring pathways that mediate inhibition of inflammatory responses. PMID: 24585880
  92. NGF deprivation, Akt dephosphorylation/inactivation, FoxO dephosphorylation/activation and Trib3 induction are linked in a self-amplifying feed-forward loop that culminates in neuron death. PMID: 24212932
  93. results suggest that NGF produced locally in the peritoneal cavity may be involved in the generation of endometriosis-associated pelvic pain PMID: 23883529
  94. The novelty of the study lies in its large number of exclusively adolescent depression patients showing significant reduction of BDNF, NGF and GDNF serum levels as compared to controls. A gender bias with much reduction in female has also been recorded. PMID: 23769609
  95. NGF levels are significantly higher in children with overactive bladder than controls PMID: 22789557
  96. Suggest that nerve growth factor, which is elevated in the failing human heart, causes hypertrophy of neurons in cardiac ganglia. PMID: 23959444
  97. Urine marker NGF may predict urinary tract impairment in children with myelodysplasia. and may differentiate at risk patients with either renal scarring or high detrusor leak point pressure. PMID: 23973519
  98. NGF expression positively correlates with itching sensation and number of nerve fibers in the epidermis in prurigo nodularis PMID: 23398122
  99. mechanical stimulation may attenuate NGFbeta signaling through Rac1 PMID: 23989259
  100. Findings indicate taht COX-2 inhibitors exaggerated nerve growth factor (NGF) expression, whereas dexamethasone inhibited it. PMID: 23574818

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Involvement in disease Neuropathy, hereditary sensory and autonomic, 5 (HSAN5)
Subcellular Location Secreted
Protein Families NGF-beta family
Database Links

HGNC: 7808

OMIM: 162030

KEGG: hsa:4803

STRING: 9606.ENSP00000358525

UniGene: Hs.2561

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