Code | CSB-E04683h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
The HumanNGF ELISA kit is designed for the detection and quantification of human NGF in serum, plasma, cell culture supernates, tissue homogenates, and cell lysates. Many performances including specificity, sensitivity, precision, linearity, and recovery of this kit, have been rigorously tested. More validation information is elucidated in the product instructions. The NGF in the sample or standard is immobilized on the pre-coated NGF antibody and then sandwiched by Biotin-conjugated NGF antibody. The forming Ag-Ab-Ag immune complex is labeled by HRP-avidin and develops a color reaction upon adding TMB substrate solution. The color intensity is positively correlated to the amount of NGF bound in the initial step and can be measured at 450 nm via a microplate reader.
NGF is a neurotrophic factor essential for the growth, differentiation, and survival of sympathetic and sensory afferent neurons during development. It contributes to neuronal phenotype by regulating axonal guidance, gene transcription, neurotransmitter release, and synaptic plasticity. Numerous studies have shown that NGF plays a pivotal role in the modulation of nociception in adulthood. There are two receptors for NGF: p75 neurotrophin receptor (p75NTR) and tropomyosin receptor kinase A (TrkA). A study demonstrated that NGF-TrkA signaling-mediated communication between osteoblasts and sensory nerves is essential for load-induced bone formation in mice.
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