Product Details

Purity

>96% as determined by SDSPAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF?receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0x10⁶ IU/mg.

Target Names

FGF4

Uniprot No.

P08620

Research Area

Cancer

Alternative Names

FGF-4; Fgf4; FGF4_HUMAN; Fibroblast growth factor 4; fibroblast growth factor 4 splice isoform; HBGF-4; HBGF4; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; Hst; HST-1; HST1; HSTF-1; HSTF1; Human stomach cancer transforming factor from FGF related oncogene; K FGF; Kaposi Sarcoma Oncogene; KFGF; KS3; Oncogene HST; Transforming protein KS3

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

25-206aa

Complete Sequence

GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL

Mol. Weight

19.8 kDa

Protein Length

Partial

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4, 300 mM NaCl

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

FGF4 plays a critical role in embryonic development, angiogenesis, and tumor progression by signaling through FGFR1–FGFR4, making it a key target in both stem cell biology and cancer research. This tag-free recombinant human FGF4 (residues 25–206) encompasses the mature secreted form and demonstrates exceptional receptor-binding potency, with an ED50 below 0.5 ng/ml in a thymidine uptake assay using FGFR-transfected BaF3 cells, corresponding to a specific activity exceeding 2.0 × 10⁶ IU/mg — providing a reliable standard for cell proliferation assays, receptor-ligand binding studies via SPR or ELISA, and angiogenesis models. Produced in *E. coli*, the protein lacks glycosylation, which offers a defined, homogeneous preparation well suited for quantitative binding competition experiments where post-translational variability could confound results. Purity exceeding 96% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for in vivo tumor biology models and stem cell differentiation studies.

Target Background

Function

Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal limb and cardiac valve development during embryogenesis.

Gene References into Functions

our study demonstrated that FGFR4 rs2011077 and rs1966265 are associated with the progression of cervical normal tissues to precancerous lesions in Taiwanese women. Moreover, rs351855 (Gly388Arg) is the only FGFR4 genetic polymorphism that is associated with patient survival. PMID: 28378614
Thus, we conclude that the oncoprotein HBXIP up-regulates FGF4 through activating transcriptional factor Sp1 to promote the migration of breast cancer cells. Therapeutically, HBXIP may serve as a novel target in breast cancer. PMID: 26828265
Fibroblasts induce expression of FGF4 in ovarian cancer stem-like cells/cancer-initiating cells and upregulate their tumor initiation capacity. PMID: 25329002
Data show that the interaction between Artd1 and Sox2 is crucial for the first steps of the reprogramming process and that early expression of Fgf4 is an essential component for the successful generation of iPSCs. PMID: 23939864
Myoblasts which overexpress FGF-4 exhibit significant changes in cell cycle and pro-angiogenic potential with only slight differences in the expression of the myogenic genes. PMID: 21673370
In vivo stimulation of BT-474 cell growth by progesterone is associated with up-regulation of FGF4 which may promote tumor growth and maintenance. PMID: 22237711
knockdown of FGFR4 expression led to decreased proliferation and an increased rate of apoptosis in the MKN45 and SGC7901 GC cell lines PMID: 21567388
activation of human HST-1 gene in transgenic mice induces spermatogenesis and prevents adriamycin-induced testicular toxicity PMID: 11840335
Differential effects of FGF4, EGF and TGFB1 on functional development of stromal layers (progenitor cell-outputs) in acute myeloid leukemia PMID: 12163055
FGF4 is upregulated by the OCT3 transcription factor in breast cancer cells. PMID: 12841847
HST-1 protects male germ cells from apoptosis under heat-stress condition in a mouse model. PMID: 14980503
Both myeov and hst (fgf4) are normally situated approximately 475-kb apart at band 11q13, a region that is frequently amplified and overexpressed in various tumours. PMID: 17390055
FGF-4 increases the rate at which MSC proliferate and has no significant effect on MSC pluripotency PMID: 17852409
These results suggest a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at later stages of development. PMID: 18192227
Implantation of human FGF4-soaked beads is sufficient to restore expression of G1- and S-phase cell-cycle genes and S-phase progression in zebrafish sonic hedgehog (Shh) mutant fin buds. PMID: 18811955
The combined action of retinoic acid and FGF4 results in induction of PDX1+ foregut endoderm. PMID: 19277121

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Subcellular Location

Secreted.

Protein Families

Heparin-binding growth factors family

Database Links

HGNC: 3682

OMIM: 164980

KEGG: hsa:2249

STRING: 9606.ENSP00000168712

UniGene: Hs.1755

Code	CSB-AP002411HU
Abbreviation	Recombinant Human FGF4 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Human Fibroblast growth factor 4 protein (FGF4), partial (Active)

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Target Background

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