Recombinant Human Interferon gamma(IFNG) (Active)

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Code CSB-AP004201HU
Size US$669Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity The ED50 as determined by a viral resistance assay using HT-29 cells is 17 pg/mL
Target Names IFNG
Uniprot No. P01579
Research Area Immunology
Alternative Names Interferon Gamma; IFN-Gamma; Immune Interferon; IFNG
Species Homo sapiens (Human)
Source E.coli
Expression Region 24-166aa
Complete Sequence QDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRRASQ
Mol. Weight 16.88 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 250mM NaCl, pH 8.5
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Gene References into Functions
  1. Impaired IFNgamma-Signaling and Mycobacterial Clearance in IFNgammaR1-Deficient Human iPSC-Derived Macrophages. PMID: 29249666
  2. High expression of IFNG is associated with breast cancer. PMID: 30336781
  3. results suggest, the interplay of pro-inflammatory cytokines IFN-gamma derived from CD4+T lymphocytes and TNF-alpha from CD14+ cells has no direct additive impact on parasite replication but induces IL-4 production. PMID: 29953494
  4. IFN-lambda may participate in local inflammation in the salivary glands of primary Sjogren's syndrome patients through direct and indirect regulations of the expressions of BAFF and CXCL10 in salivary gland epithelium. PMID: 28421993
  5. The expression of CXCL10 mRNA and IFN-gamma mRNA was significantly higher in non-lesional and perilesional skin of vitiligo and alopecia areata patients compared with the skin of healthy controls; however, the level of expression of CXCL10 and IFN-gamma in lesional skin was not different than that in healthy skin PMID: 27863059
  6. High IFNG expression is associated with Chronic Periodontitis. PMID: 30051674
  7. hypomethylation of the IFNG promoter is significantly related to the risk of essential hypertension PMID: 29643275
  8. These results demonstrated that the IFNGinduced immunosuppressive properties of B7H1 in human BM and WJMSCs were mediated by STAT1 signaling, and not by PI3K/RACalpha serine/threonineprotein kinase signaling PMID: 29901104
  9. serum IP-10 level and the IFN-gamma/IL-4 ratio have great potential to predict significant fibrosis among chronic hepatitis B patients. PMID: 28067328
  10. IFN-gamma increases free ISG15 levels in the cytoplasm and ISGylation in the nucleus and cytoplasm, but in a manner distinct between MCF-7 and MDA-MB-231cells. PMID: 29626479
  11. the expression of IFN-gamma and IL-17 was also suppressed by IRAK1/4 inhibitor both in active Behcet's patients and in normal subjects. PMID: 28780618
  12. IFNgamma induces a rapid activation of aerobic glycolysis followed by a reduction in oxidative phosphorylation in M1 macrophages. PMID: 29463472
  13. Results provide evidence that rs2069707 locus SNPs of IFN-gamma is a risk factor for contracting tuberculous pericarditis. PMID: 30017738
  14. No correlation was observed between interferon gamma mRNA/protein levels and recurrent depressive disorders. PMID: 29367100
  15. Aberrant IFN-gamma promoter methylation may be involved in the process of tumorigenesis of oral cancer. PMID: 28091876
  16. this study shows that elevated levels of interferon-gamma are associated with high levels of Epstein-Barr virus reactivation in patients with the intestinal type of gastric cancer PMID: 29349089
  17. This study contributes to clarification of the previously inconsistent prognostic performance of IFNgamma by providing the first prognostic evaluation with long follow-up, time-dependence assessment and absence of any chemotherapy influence. PMID: 29478965
  18. Association Between the Interferon Gamma 874 T/A Polymorphism and the Severity of Valvular Damage in Patients with Rheumatic Heart Disease. PMID: 29332266
  19. IFN-gamma can promote cancer immunoevasion. (Review) PMID: 29283429
  20. an electrophoretic mobility shift assay showed that signal transducers and activators of transcription 1 (STAT1) attach to the GAS motif on the human STING promoter region. This indicates that IFN-gamma/Janus kinases/STAT1 signaling is essential for the STING upregulation in human keratinocytes. PMID: 29143896
  21. we review the direct and indirect effects of IFN-gamma on hematopoiesis, as well as the underlying signaling mechanisms of how IFN-gamma modulates the self-renewal, cell cycle entry, and proliferation of hematopoietic stem cells PMID: 28852997
  22. IFN-gamma +874T allele may increase the risk of ocular lesions in Toxoplasma infection. The principle of natural selection seems to also play a role. The less common TNF-308A allelic form could be protective against the development of Toxoplasma ocular infection. PMID: 27081842
  23. this study shows the age-related reductions in serum and PBMC IFN-gamma in healthy nonobese subjects PMID: 28762199
  24. Phosphorylation of T-bet by RSK2 is required for IFNgamma expression for attenuation of colon cancer metastasis and growth. PMID: 29133416
  25. SNX8 mediates IFNG-triggered non-canonical signaling pathway and host defense against Listeria monocytogenes. PMID: 29180417
  26. the frequencies of IFNgamma and IL-17A(+) cells were increased in the antrum, particularly in patients with H. pylori induced gastric ulcers. PMID: 28683359
  27. rs2069718 in the IFNG gene was significantly associated with pulmonary tuberculosis but not spinal tuberculosis. PMID: 28867622
  28. IFN-gamma was associated with a cerebral volume reduction in systemic lupus erythematosus with central nervous system involvement PMID: 28848179
  29. Data suggest that semen exhibits substantial individual variation over time in pro-inflammatory seminal fluid cytokines IFNG and CXCL8. (IFNG = interferon gamma; CXCL8 = C-X-C motif chemokine ligand 8) PMID: 28541460
  30. Dysregulation of the IFN-gamma-STAT1 signaling pathway in a cell line model of large granular lymphocyte leukemia PMID: 29474442
  31. STAT1b plays a key role in enhancing the tumor suppressor function of STAT1a, in ESCC, in a manner that can be amplified by IFN-gamma PMID: 28981100
  32. epigenetic silencing by single CpG methylation determines differential IL18BP inducibility in monocytic versus epithelial cells. T PMID: 29409936
  33. Systemic IFN activation is associated with higher activity only in the ESSDAI biological domain but not in other domains or the total score. Our data raise the possibility that the ESSDAI biological domain score may be a more sensitive endpoint for trials targeting either IFN pathway. PMID: 29474655
  34. These findings suggest that IFN-a can inhibit HCV replication through a STAT2-dependent but STAT1-independent pathway, whereas IFN-g induces ISG expression and inhibits HCV replication exclusively through a STAT1- and STAT2-dependent pathway. PMID: 27929099
  35. rs1861493 and rs2430561 polymorphisms were conformed to be in HWE in genotypes distribution of the control group (P>0.05 for both). However, only TT genotype and T allele of rs2430561 presented significantly higher frequencies in Ankylosing Spondylitis patients than in healthy controls. IFN-g rs2430561 polymorphism may contribute to the risk of IFN-g rs2430561 polymorphism may contributhrough influencing IFN-g expression. PMID: 28843049
  36. Interactions among polymorphisms of IFN-gamma+874 AA, IL-2-330 TT, IL-10-1082 AA, IL10--592 AC and IL-4-589 CC/CT significantly influenced the clinical progression of the subjects with hepatitis B virus and/or hepatitis C virus infection. PMID: 28838891
  37. Ex vivo interferon-gamma production is a useful biomarker for assessing disease activity and predicting poor clinical outcomes of systemic lupus erythematosus patients. PMID: 28841837
  38. this study demonstrates reduced IFN-gamma production in chronic brucellosis patients PMID: 28919584
  39. These data suggest that the de novo expression of PDL1 on tumor cells is upregulated by IFN-g secreted from CD8+ TILs upon recognition of the tumor cells with an MHC class I molecule. PMID: 28791392
  40. H pylori expression of cgt reduces cholesterol levels in infected gastric epithelial cells and thereby blocks IFNG signaling, allowing the bacteria to escape the host inflammatory response. PMID: 29273450
  41. Strategies to block MICA-NKG2D interactions resulted in reductions in IFNgamma production. Depletion of monocytes in vivo resulted in decreased IFNgamma production by murine NK cells upon exposure to Ab-coated tumor cells PMID: 28724544
  42. study concluded that the IFN-gamma (874A/T) polymorphism is associated with the susceptibility to oral lichen planus PMID: 27544215
  43. IFN gamma induced upregulation of BCL6 was dependent on the classical STAT1 signaling pathway, and affected both major BCL6 variants. Interestingly, although IFN alpha induced stronger STAT1 phosphorylation than IFN gamma, it only slightly upregulated BCL6 in multiple myeloma lines. PMID: 29510136
  44. IFN-gamma, CXCL16 and uPAR are promising as effective biomarkers of disease activity, renal damage, and the activity of pathological lesions in systemic lupus erythematosus. PMID: 28628472
  45. serum levels of soluble FAS ligand (sFASL) and interferon gamma (IFN-gamma) were analyzed and correlated with sFGL2 levels in Hepatitis C Virus-Infected Patients and Hepatocellular Carcinoma Patients. PMID: 28609212
  46. IFN-gamma induces activated but insufficient autophagy and thus contributes to a degree to p62-dependent apoptosis of nasal epithelial cells in chronic rhinosinusitis with nasal polyps. PMID: 28258963
  47. results suggested that IFN-gamma induces autophagy-associated apoptosis in CRC cells via inducing cPLA2-dependent mitochondrial ROS production. PMID: 29551681
  48. Activated interferon-gamma-producing CD56(bright) NK cells are positioned to play a key role in the fibrotic process and progression to chronic kidney disease. PMID: 28396119
  49. Posttransplant immune monitoring by donor-specific IFN-gamma ELISPOT can assess risk for developing subclinical T-cell mediated rejection and anti-donor HLA antibodies. PMID: 28274484
  50. genetic polymorphism is not associated with increased susceptibility to chronic spontaneous urticaria in Iran PMID: 28159384

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Involvement in disease Aplastic anemia (AA)
Subcellular Location Secreted
Protein Families Type II (or gamma) interferon family
Tissue Specificity Released primarily from activated T lymphocytes.
Database Links

HGNC: 5438

OMIM: 147570

KEGG: hsa:3458

STRING: 9606.ENSP00000229135

UniGene: Hs.856

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