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| Code | CSB-MP004937HU3 |
| Size | $368 How to order? |
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| Description |
Human CD40 ligand (CD40LG) amino acid Met13-Leu261, with an N-terminal hFc-Flag-tag as well as an N-terminal linker, was expressed in mammalian cells. The product is the recombinant human CD40LG protein. The SDS-PAGE analysis determined its purity greater than 90% and showed a molecular weight band of about 45 kDa on the gel. It contains low levels of endotoxin, less than 1.0 EU/ug measured by the LAL method. Functional ELISA and LSPR assay of this CD40LG protein showed that it is an active protein. This protein is in stock so that it will reach your lab bench faster. CD40LG, also known as CD154, is a ligand for the CD40. It is preferentially expressed by activated CD4+ T cells as well as activated B cells and platelets. The interaction of CD40 and CD40LG plays an essential role in immune regulation and homeostasis. The CD40-CD40L system is also involved in the pathophysiology of several diseases, including autoimmune diseases, atherothrombosis, respiratory disease, and cancer. |
| Purity | Greater than 93% as determined by SDS-PAGE. |
| Endotoxin | Less than 1.0 EU/ug as determined by LAL method. |
| Activity | ①Measured by its binding ability in a functional ELISA. Immobilized CD40L at 2 μg/ml can bind Anti- CD40L Rabbit Monoclonal Antibody(CSB-RA156386A0HU), the EC50 is 2.353-3.232 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized CD40(CSB-MP004936HU1) at 2 μg/ml can bind CD40L, the EC50 is 3.112-3.858 ng/ml. ③Human CD40 protein hFc tag (CSB-MP004936HU1) captured on COOH chip can bind Human CD40L protein hFc and Flag tag (CSB-MP004937HU3) with an affinity constant of 2.06 nM as detected by LSPR Assay. |
| Target Names | CD40LG |
| Uniprot No. | P29965 |
| Research Area | Cancer |
| Alternative Names | (CD40-L)(T-cell antigen Gp39)(NF-related activation protein)(TRAP)(Tumor necrosis factor ligand superfamily member 5)(CD154)(sCD40L) |
| Molecular Characterization |  |
| Species | Homo sapiens (Human) |
| Source | Mammalian cell |
| Expression Region | 113-261aa |
| Target Protein Sequence | MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL |
| Mol. Weight | 44.5 kDa |
| Protein Length | Partial |
| Tag Info |
N-terminal hFc-Flag-tagged |
| Form |
Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4 |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching.; Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
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| Gene References into Functions |
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| Involvement in disease | Immunodeficiency with hyper-IgM, type 1 (HIGM1) |
| Subcellular Location | Cell membrane; Single-pass type II membrane protein. Cell surface.; [CD40 ligand, soluble form]: Secreted. |
| Protein Families | Tumor necrosis factor family |
| Tissue Specificity | Specifically expressed on activated CD4+ T-lymphocytes. |
| Database Links |
HGNC: 11935 OMIM: 300386 KEGG: hsa:959 STRING: 9606.ENSP00000359663 UniGene: Hs.592244 |
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