Product Details

Purity

Greater than 90% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

①Measured by its binding ability in a functional ELISA. Immobilized human BCMA (CSB-MP023974HU1) at 5 μg/ml can bind Biotinylated human TNFSF13B, the EC₅₀ is 0.1752-0.3657 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF13C (CSB-MP853495HU) at 2 μg/ml can bind Biotinylated human TNFSF13B, the EC₅₀ is 0.2699-0.5613 ng/ml.

Target Names

TNFSF13B

Uniprot No.

Q9Y275

Alternative Names

B lymphocyte stimulator （BLyS）（B-cell-activating factor）（BAFF）（Dendritic cell-derived TNF-like molecule）（TNF- and APOL-related leukocyte expressed ligand 1）（TALL-1）

Molecular Characterization

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

134-285aa

Target Protein Sequence

AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL

Mol. Weight

46.2 kDa

Protein Length

Partial

Tag Info

N-terminal hFc-Avi-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant human TNFSF13B was produced in mammalian cells. The DNA fragment used to prepare the recombinant TNFSF13B protein corresponds to amino acid 134-285 of the human TNFSF13B protein containing an N-terminal hFc-Avi-tag. This TNFSF13B protein is an active protein, whose bio-activity has been validated in the functional ELISA. It binds to the human BCMA or human TNFRSF13C, with the EC₅₀ of 0.1752-0.3657 ng/ml or 0.2699-0.5613 ng/ml, respectively. Its purity reaches up to 90%, determined by SDS-PAGE. This TNFSF13B protein has an apparent molecular mass of 46 kDa on the gel while its predicted mass is 46.2 kDa. Its endotoxin is less than 1.0 EU/ug measured by the LAL method. It is available now.

TNFSF13B, also called BAFF, modulates B cell survival, maturation, and differentiation by binding to its receptors, BAFF-R, TACI, and BCMA. Upregulation of BAFF is related to autoimmune diseases, including systemic lupus erythematosus, rheumatoid arthritis, and multiple myeloma. BAFF/BAFFR signaling is involved in the regulation of protein synthesis and energy metabolism required to extend the half-life of immature, transitional, and mature B cells, as well as other survival functions.

Target Background

Function

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.; Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.; Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis.

Gene References into Functions

BAFF loop region controls B cell survival and regulates recognition by different inhibitors PMID: 29572442
analysis of how BAFF is neutralized by belimumab gives insight into treatment of systemic lupus erythematosus PMID: 29572471
Study uncovers a previously unrecognized regulation of BAFF by testosterone and raises important questions about BAFF in testosterone-mediated protection against autoimmunity. Among healthy men, serum BAFF levels are higher in men with low testosterone. PMID: 29802242
High BAFF expression is associated with systemic lupus erythematosus. PMID: 28992184
Co-immunoprecipitation analysis and siRNA-mediated suppression of CREB expression indicated that phospho-CREB has a positive effect on pro-inflammatory gene expression in the crosstalk between BAFF- and TLR4-mediated signaling by forming trimeric complexes containing NF-kappaB, CBP, and CREB PMID: 28374824
this study shows that BAFF augments IgA2 and IL-10 production by TLR7/8 stimulated total peripheral blood B cells PMID: 28921509
elevated pretransplant serum BAFF levels negatively affect renal allograft survival and represent a risk factor for allosensitization and subsequent antibody-mediated rejection PMID: 29277566
Increased BAFF expression is associated with B cell class switching in patients with tuberculous pleural effusion. PMID: 29845274
Expression patterns of BAFF and its receptors differ according to lupus nephritis class. PMID: 29087261
Serum BAFF levels are elevated in idiopathic inflammatory myositis, more so in children. PMID: 29516280
Post-transplant antibody mediated rejection in kidney transplantation recipients can be predicted by perioperative elevations in serum BAFF level. PMID: 27888573
The results of the present study revealed a correlation between BAFF and the PI3K/Akt/mTOR signaling pathway, and it is hypothesized that they are involved in the pathogenesis of lupus nephritis PMID: 28849060
The results suggest that increased levels of BAFF and APRIL produced in the central nervous system may influence the development of anti-neutrophil cytoplasmic antibody-hypertrophic pachymeningitis. PMID: 28847534
Blood B lymphocyte stimulator (BLyS)/BAFF levels of HIV-uninfected commercial sex workers (CSWs) were lower than those observed in both HIV-infected CSW and HIV-uninfected non-CSW groups. PMID: 27561453
our study throws light on the crosstalk between BAFF and BCR signaling pathways in neoplastic B cells, and provides insights into the mechanistic effects of SYK inhibitors in CLL. PMID: 28838991
genetic polymorphisms of BAFF may increase the risk of posttransplant development of donor specific antibodies in kidney allograft recipients PMID: 28624489
P\pre-sensitized patients had significantly higher BAFF levels before transplantation and suffered significantly more often from early steroid-resistant, mainly antibody-mediated rejections PMID: 28867309
Elevated blood BAFF levels could be associated with a more stable disease. PMID: 27383531
BAFF rs9514828 polymorphism may be associated with the chronic hepatitis and the combinatorial action of rs9514828 and rs12583006 may confer susceptibility to chronic HBV infection and the resolution of the infection, suggesting that host genetic factors associated with B cell mediated immune responses are involved in chronic HBV infection. PMID: 28627389
This study demonstrated that An Increase of Cerebrospinal Fluid B-cell Activating Factor Level in Pediatric Patients With Acute Viral Encephalitis. PMID: 28259511
data show that BAFF levels at the time of cGvHD diagnosis are associated with non-relapse-mortality, and also are potentially useful for risk stratification. PMID: 28481353
BAFF-R was consistently expressed on B cells infected by HCMV. Enhancement of BAFF/BAFF-R signaling decreased the apoptosis rate and extended the survival of B cells. PMID: 28442365
soluble BCMA sequesters circulating BAFF, thereby preventing it from performing its signaling to stimulate normal B-cell and plasma cell development, resulting in reduced polyclonal antibody levels in multiple myeloma patients. PMID: 26960399
up-regulated expression in intractable temporal lobe epilepsy PMID: 28441631
results showed that serum BAFF in nasal type, extranodal NK/T cell lymphoma patients was significantly higher than that in control group and negatively correlated with patients' survival. PMID: 27668971
BAFF has a role in inducing IL35 production by regulatory B cells in lupus PMID: 28844943
Inhibition of ADAM10 augments BAFF-dependent survival of primary human B cells, whereas inhibition of ADAM17 increases BAFFR expression levels PMID: 28249164
Data presented show that B-cell activating factor (BAFF) plays a central role in the induction and maintenance of cigarette smoke-induced pulmonary antinuclear antibodies and suggest a therapeutic potential for BAFF blockade in limiting autoimmune processes associated with smoking. PMID: 28039405
Among the BAFF receptors in a cohort of rheumatoid arthritis (RA) patients, the AA have shown, by fluorescence activated cell sorter (FACS) analysis of median fluorescence intensity (MFI), that transmembrane activator and calcium-modulating cyclophilin ligand interactor (TACI) and B cell maturation antigen (BCMA) do not change PMID: 28834574
The BAFF promoter increased in response to TNF-alpha treatment or overexpression of HIF-1alpha. However, TNF-alpha-induced BAFF expression and promoter activity decreased after treatment with the ERK inhibitor PD98059. PMID: 28383556
This study indicates that orbital fibroblasts from Graves' orbitopathy can express BAFF and mediate the intraorbital survival of B cells via BAFF mechanism. PMID: 28087387
Rapamycin attenuates excessive hsBAFF-induced cell proliferation/survival via blocking mTORC1/2 signaling in normal and neoplastic B-lymphoid cells. PMID: 28300280
Inhibition of B cell plasmablast differentiation by reduction of Aiolos and Ikaros may have utility in the treatment of systemic lupus erythematosus , where elevated levels of BAFF and Aiolos may prime CD27(+) memory and double negative memory-like B cells to become Ab-producing plasmablasts in the presence of BAFF and proinflammatory cytokines. PMID: 28848067
BCMA has other contributors for ligands binding except DxL motif. The affinity of BCMA for APRIL higher than for BAFF may be caused by the segment outside of the conservative DxL motif. Moreover, the exposition of new binding modes of BCMA2 interacting with APRIL may establish the foundation of designing novel drugs in the future PMID: 28260502
study demonstrated a high prevalence of endogenous antibodies to BAFF in a multi-ethnic Asian systemic lupus erythematosus (SLE) cohort; while levels of serum BAFF correlated positively with disease activity, levels of anti-BAFF antibody were correlated negatively with levels of its target cytokine, anti-dsDNA antibody and clinical disease activity PMID: 28388832
this study provides new useful information about the increased levels of BAFF observed during HIV-1 infection and highlights the importance of macrophages as a source of BAFF PMID: 27022194
The novel association between BAFF and inflammatory bowel disease (IBD) seems to identify that BAFF might regulate the inflammatory process in these diseases and it appears to be a potential marker of IBD. PMID: 27056038
In BAFF, rs2893321 may be a susceptible genetic variant for the development of GD and AITDs. Associations of rs2893321 with susceptibility to GD and AITDs and the correlation between rs2893321 and TAb exhibit a dimorphic pattern. Additional studies with larger sample sizes are required to confirm our findings. PMID: 27136204
BAFF and IL-17A are associated with different subphenotypes of primary Sjogren's syndrome. PMID: 25941062
The expression levels of serum BAFF and the three receptors (TACI, BCMA and BAFF-R) in non-Hodgkin lymphoma patients were significantly higher than in healthy controls. PMID: 28028945
Plasma BAFF levels were positively associated with serum creatinine, proteinuria, uric acid and group A Streptococcus infection index in patients with IgA nephropathy. PMID: 28260100
this study shows that chicoric acid suppresses BAFF expression by inhibiting NF-kappaB activity, and chicoric acid may serve as a novel therapeutic agent to down-regulate excessive BAFF expression in autoimmune diseases PMID: 28122293
Findings indicate that BAFF expression is significantly increased in chronic rhinosinusitis with nasal polyps patients and may orchestrate inflammatory load in polyp tissues by regulating T and B cell-mediated response. PMID: 28035475
Urinary APRIL (uAPRIL) and BAFF (uBAFF) levels were raised significantly in AN. PMID: 27804111
BAFF levels are lower in patients with antibody-mediated kidney rejection and also in patients with concurrent humoral and cellular rejection compared with patients without rejection PMID: 28083608
The BLyS level is increased in some lupus patients. There was a moderate correlation with titers of anti-DNA antibody and disease activity. PMID: 27100979
In Sjogren's syndrome (SS) patients, EULAR Sjogren's syndrome disease activity index (ESSDAI) is negatively associated with serum levels of 25(OH)-D3 and positively associated with BAFF. PMID: 28074193
These results suggest that miR-202 functions as a modulator that can negatively regulate BAFF by inhibiting multiple myeloma tumor cell survival, growth, and adhesion in the bone marrow microenvironment. PMID: 25971527
Variants in BAFF gene is associated with chronic lymphocytic leukemia. PMID: 27468724
The results demonstrated that BAFF has an important role in the pathogenesis of newly diagnosed childhood immune thrombocytopenia. PMID: 24911453

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Subcellular Location

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor ligand superfamily member 13b, soluble form]: Secreted.

Protein Families

Tumor necrosis factor family

Tissue Specificity

Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver,

Database Links

HGNC: 11929

OMIM: 603969

KEGG: hsa:10673

STRING: 9606.ENSP00000365048

UniGene: Hs.525157

Code	CSB-MP897523HU1-B
Abbreviation	Recombinant Human TNFSF13B protein, partial, Biotinylated (Active)
MSDS	MSDS Datasheet
Size	$338
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized human BCMA (CSB-MP023974HU1) at 5 μg/ml can bind Biotinylated human TNFSF13B, the EC₅₀ is 0.1752-0.3657 ng/ml. Biological Activity Assay Activity Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF13C (CSB-MP853495HU) at 2 μg/ml can bind Biotinylated human TNFSF13B, the EC₅₀ is 0.2699-0.5613 ng/ml. Biological Activity Assay The purity of TNFSF13B was greater than 90% as determined by SEC-HPLC.
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Recombinant Human Tumor necrosis factor ligand superfamily member 13B (TNFSF13B), partial,Biotinylated (Active)

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