GAPDH Antibody

Datasheet
Code CSB-PA00025A0Rb
See More Details Free Antibody trial simple
Size US$299Purchase it in Cusabio online store
(Shop online in the US. Customers outside the US,
please order from local distributors.)
Uniprot No. P04406
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
    All lanes: GAPDH antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 37, 32 kDa
    Observed band size: 37 kDa

  • Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA00025A0Rb at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA00025A0Rb at dilution of 1:100

  • Immunofluorescent analysis of MCF-7 cells using CSB-PA00025A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA00025A0Rb at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00025A0Rb at dilution of 1:100

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase protein (3-335AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Relevance Glycerol 3 Phosphate Dehydrogenase is a homodimer and belongs to the NAD dependent Glycerol 3 Phosphate Dehydrogenase family.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-), GAPDH, GAPD
Immunogen Species Human
Research Area Neuroscience
Target Names GAPDH
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
References
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Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Subcellular Location Cytoplasm, cytosol, Nucleus, Cytoplasm, perinuclear region, Membrane, Cytoplasm, cytoskeleton
Protein Families Glyceraldehyde-3-phosphate dehydrogenase family
Database Links

HGNC: 4141

OMIM: 138400

KEGG: hsa:2597

STRING: 9606.ENSP00000229239

UniGene: Hs.544577

Pathway HIF-1 signaling pathway

Q&A and Customer Reviews

Customer Reviews
  • Applications: WB
    Review: The expression levels of caspase-3, active caspase-3, p62 and LC3B in control and PGRMC1 knockdown hPSCs. β-actin was used as internal protein control and loading control. Full-length blots are presented in Supplementary Figure 8. Images are representative of at least three independent experiments.
    PMID: 29445107
  • Applications: WB
    Review: Western blotting of the bladder cancer cells confirmed the expression of E-cadherin (epithelial marker) in SW780 and RT112 (female low and intermediate grade) and highest level of Ncadherin (mesenchymal marker) in T24 (female high grade) followed by male bladder cancer cells, which is an indicative of their respective EMT status. For all bar graphs, error bars represent SEM. Statistical significance computed using one-way ANOVA for 5a and unpaired parametric t-test for 5b and c is given by *P < 0.05; **P < 0.01.
  • Applications: WB
    Antibody dilution factor: 1:10,000
    Review: Expression and phosphorylation analysis of PGRMC1, GSK-3β, β-catenin, and Wnt3a in control or PGRMC1 knockdown hPSCs. Cell lysates were analyzed by Western blot analysis with indicated antibodies. GAPDH was used as internal protein control and loading control.
    PMID: 30992425
  • Applications:WB
    Review: ADRP expression level from (k) was determined using the ImageJ software and was normalized using the GAPDH expression level; **p < 0.01.
    PMID: 31086253
  • Applications: WB
    Review: Inhibition of human CML cell growth by using GT. U937, HL-60, and K562 cells were treated with various GT concentrations (200–400 mg/mL) for 24 h. (A) Cell viability before and after GT treatment was determined through MTT assay. (B) Phase-contrast microscopy (200× magnification) was used to examine structural changes in GT-treated K562 cells. Data are reported as the mean ± standard deviation (SD) of three experiments. *p < 0.05; **p < 0.01; ***p < 0.001 relative to untreated (control) cells.
    PMID: 30465897
  • Applications: WB
    Review: The western blotting results of MMP-2 and MMP-9 are shown in Figure 5A,B, respectively. The addition of 5 M FXT increased the expression of MMP-2 and MMP-9 protein significantly by about 2.0-fold and 75%, respectively, compared with control. The co-treatment of 30 and 60 M GNP- 5 M FXT decreased the MMP-2 protein expression by about 30% and 42%, respectively, compared with the FXT treatment alone. A similar trend was observed for MMP-9 where the protein expression decreased by about 23% and 43%, respectively. The results for protein expression of TIMP-1 and TIMP-2 in HepG2 cells are shown in Figure 5C,D, respectively. The addition of 5 M FXT decreased the protein expression of TIMP-1 and TIMP-2 by about 55% and 36%, respectively, compared to control. However, with the co-treatment of 30 and 60 GNP with 5 M FXT, the TIMP-1 protein expression increased significantly by about 78% and 1.3-fold, respectively, compared to FXT treatment alone. A similar trend was observed for TIMP-2 protein expression where it increased about by 28% and 67%, respectively (p < 0.05).
    PMID: 30558243

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