GAPDH Antibody

Datasheet
Code CSB-PA00025A0Rb
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Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. P04406
Image
  • Western blot
    All lanes: Glyceraldehyde-3-phosphate dehydrogenase antibody at 2μg/ml
    Lane 1: Hela whole cell lysate
    Lane 2: A549 whole cell lysate
    Lane 3: HepG2 whole cell lysate
    Lane 4: K562 whole cell lysate
    Lane 5: Jurkats whole cell lysate
    Lane 6: MDA-MB-231 whole cell lysate
    Lane 7: LO2 whole cell lysate
    Lane 8: U251 whole cell lysate
    Lane 9: MCF-7 whole cell lysate
    Secondary
    Goat polyclonal to Rabbit IgG at 1/15000 dilution
    Predicted band size: 37, 32 kDa
    Observed band size: 37 kDa

  • Western blot
    All lanes: GAPDH antibody at 2μg/ml
    Lane 1: Hela whole cell lysate
    Lane 2: 293T whole cell lysate
    Lane 3: Jurkat whole cell lysate
    Lane 4: Mouse kidney tissue
    Lane 5: Rat muscle tissue
    Lane 6: Rat lung tissue
    Lane 7: Zebrafish lysate
    Secondary
    Goat polyclonal to Rabbit IgG at 1/10000 dilution
    Predicted band size: 37, 32 kDa
    Observed band size: 37 kDa

  • Western Blot
    Positive WB detected in: HepG2 cell, Mouse brain tissue, Mouse skeletal muscle tissue
    All lanes: GAPDH antibody at 2.6μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 37, 32 kDa
    Observed band size: 37 kDa

  • Western Blot
    Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
    All lanes: GAPDH antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 37, 32 kDa
    Observed band size: 37 kDa

  • Immunohistochemistry of paraffin-embedded Mouse liver tissue using CSB-PA00025A0Rb at dilution of 1:50

  • Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA00025A0Rb at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA00025A0Rb at dilution of 1:100

  • Immunofluorescent analysis of MCF-7 cells using CSB-PA00025A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunogen Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase protein (3-105AA)
Raised in Rabbit
Species Reactivity Human, Mouse, Rat, Zebrafish
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Relevance Glycerol 3 Phosphate Dehydrogenase is a homodimer and belongs to the NAD dependent Glycerol 3 Phosphate Dehydrogenase family.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-), GAPDH, GAPD
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Neuroscience
Target Names GAPDH
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
References
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Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Subcellular Location Cytoplasm, cytosol, Nucleus, Cytoplasm, perinuclear region, Membrane, Cytoplasm, cytoskeleton
Protein Families Glyceraldehyde-3-phosphate dehydrogenase family
Database Links

HGNC: 4141

OMIM: 138400

KEGG: hsa:2597

STRING: 9606.ENSP00000229239

UniGene: Hs.544577

Pathway HIF-1 signaling pathway

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